Construct: sgRNA BRDN0001146546

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATTACTCACTAAAGAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKRA (8575)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76301

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 178441617 + PRKRA NNNTACTCACTAAAGAAATG NGG 0 1.0 Tier I
2 NC_000014.9 14 96286347 + ATG2B NNNTATTCACTAAAGAAATA NGG 2 0.8705 Tier II
3 NC_000002.12 2 88043459 + KRCC1 NNNTGCTCACTAAAGAAATA NGG 2 0.675 Tier II
4 NC_000017.11 17 69423854 - MAP2K6 NNNTACTTTCTAAAGAAATG NGG 2 0.525 Tier II
5 NC_000002.12 2 111967889 - MERTK NNNTATTCACTAATGAAATG NGG 2 0.4952 Tier II
6 NC_000006.12 6 131876015 + ENPP1 NNNGACTCACTGAAGAAATG NGG 2 0.4514 Tier II
7 NC_000021.9 21 41908202 + C2CD2 NNNTACACACTAAAGAATTG NGG 2 0.4375 Tier II
8 NC_000015.10 15 54539427 - UNC13C NNNAACTAACTAAAGAAATG NGG 2 0.3095 Tier II
9 NC_000001.11 1 119721006 - PHGDH NNNTACTCACTAGGGAAATG NGG 2 0.3043 Tier II
10 NC_000018.10 18 59152107 - SEC11C NNNTACTTACTTAAGAAATG NGG 2 0.2917 Tier II
11 NC_000003.12 3 197124264 - DLG1 NNNTACCCAGTAAAGAAATG NGG 2 0.2674 Tier II
12 NC_000003.12 3 61008746 + FHIT NNNTACTCACATAAGAAATG NGG 2 0.25 Tier II
13 NC_000004.12 4 15721080 + BST1 NNNGACTCAGTAAAGAAATG NGG 2 0.2431 Tier II
14 NC_000020.11 20 9222898 + PLCB4 NNNTACTCAGTAAGGAAATG NGG 2 0.1815 Tier II
15 NC_000005.10 5 62320202 - KIF2A NNNTACTCACTAATGAAATG NAG 2 0.1383 Tier II
16 NC_000001.11 1 222706102 + AIDA NNNTAGTCACTAAAGAAATG NAG 2 0.1296 Tier II
17 NC_000016.10 16 62029921 + CDH8 NNNTTCTCACTTAAGAAATG NGG 2 0.1212 Tier II
18 NC_000008.11 8 104413671 + DPYS NNNTACTCACTGAATAAATG NGG 2 0.1032 Tier II
19 NC_000015.10 15 34273511 + SLC12A6 NNNAACTCACTAAAGGAATG NGG 2 0.0916 Tier II
20 NC_000006.12 6 111102085 - SLC16A10 NNNTACTCACTAAACAAAGG NGG 2 0.0682 Tier II
21 NC_000002.12 2 237901874 + RAMP1 NNNTACTCACTACAGAAAAG NGG 2 0.0602 Tier II
22 NC_000006.12 6 165473769 + PDE10A NNNTAATCACTAAAGAAATG NTG 2 0.0362 Tier II
23 NC_000014.9 14 57600301 + SLC35F4 NNNTAATCACTAAAGAAATG NTG 2 0.0362 Tier II
24 NC_000005.10 5 79657343 - TENT2 NNNTACTAACTAAAGAAATG NTG 2 0.0253 Tier II
25 NC_000002.12 2 40149235 - SLC8A1 NNNTACTTACTAAAGAAATG NGT 2 0.0141 Tier II
26 NC_000005.10 5 109194132 + FER NNNTACCCACTAAAGAAATG NGT 2 0.0111 Tier II
27 NC_000007.14 7 133660296 - EXOC4 NNNTACTCAGTAAAGAAATG NGT 2 0.0063 Tier II
28 NC_000014.9 14 59302196 - DAAM1 NNNTACTCACTAAAGAAATG NAA 2 0.0 Tier II
29 NC_000002.12 2 220618439 - LOC105373895 NNNTACTTATTAAAGAAATG NGG 2 0.8235 Tier III
30 NC_000006.12 6 152765376 + LINC02840 NNNTATTCTCTAAAGAAATG NGG 2 0.5571 Tier III
31 NC_000004.12 4 155447242 + LOC105377503 NNNTACTCCTTAAAGAAATG NGG 2 0.5378 Tier III
32 NC_000004.12 4 159634301 + LOC107986324 NNNTACTCACTTAAGAAATA NGG 2 0.3125 Tier III
33 NC_000011.10 11 90749693 + DISC1FP1 NNNAACTCACTAAAGAAGTG NGG 2 0.1905 Tier III
34 NC_000008.11 8 101397351 - LOC107986897 NNNTACTCACTCAAGAATTG NGG 2 0.1316 Tier III
35 NC_000004.12 4 144417341 - LOC105377462 NNNTTCTCACTCAAGAAATG NGG 2 0.0957 Tier III
36 NC_000004.12 4 138328358 + LINC00499 NNNTACTCACTCAATAAATG NGG 2 0.0376 Tier III
37 NC_000014.9 14 48745924 - LOC105378178 NNNTACTCACTAAAGAAATT NTG 2 0.0273 Tier III
38 NC_000013.11 13 67580139 - LOC105370248 NNNTACACACTAAAGAAATG NGT 2 0.0141 Tier III
39 NC_000002.12 2 40149235 - SLC8A1-AS1 NNNTACTTACTAAAGAAATG NGT 2 0.0141 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 76636802 + Prkra NNNTACTTACTAGAGAAATG NGG 2 0.5707 Tier I
2 NC_000082.6 16 88145420 - Grik1 NNNTAATCACAAAAGAAATG NGG 2 0.6964 Tier II
3 NC_000075.6 9 29066697 + Ntm NNNTGCTCATTAAAGAAATG NGG 2 0.6776 Tier II
4 NC_000070.6 4 21822770 + Usp45 NNNTACTTACTGAAGAAATG NGG 2 0.6319 Tier II
5 NC_000081.6 15 96366533 + Arid2 NNNTACCCACTAAAGAATTG NGG 2 0.3438 Tier II
6 NC_000077.6 11 93129984 + Car10 NNNTACACAGTAAAGAAATG NGG 2 0.3403 Tier II
7 NC_000071.6 5 121434253 + Naa25 NNNTTATCACTAAAGAAATG NGG 2 0.3377 Tier II
8 NC_000067.6 1 68664000 - Erbb4 NNNTACTCACTGAGGAAATG NGG 2 0.337 Tier II
9 NC_000070.6 4 84612729 + Bnc2 NNNTCCTAACTAAAGAAATG NGG 2 0.325 Tier II
10 NC_000071.6 5 5165757 - Cdk14 NNNTAGGCACTAAAGAAATG NGG 2 0.2941 Tier II
11 NC_000079.6 13 105248609 - Rnf180 NNNTACACACTATAGAAATG NGG 2 0.2625 Tier II
12 NC_000070.6 4 103121880 + Mier1 NNNCACTCACTAAAGAAGTG NGG 2 0.2588 Tier II
13 NC_000069.6 3 40921181 + Abhd18 NNNTATTCACTCAAGAAATG NGG 2 0.2444 Tier II
14 NC_000070.6 4 95611252 + Fggy NNNTACTCATTAAAGAAATG NAG 2 0.244 Tier II
15 NC_000077.6 11 103687417 - Gosr2 NNNTACTTACTAAAGAAATG NAG 2 0.2269 Tier II
16 NC_000072.6 6 119785589 + Erc1 NNNTACACACTAAAGAAAGG NGG 2 0.2188 Tier II
17 NC_000082.6 16 66980239 - Cadm2 NNNTGCTCACTAAAGAAATG NAG 2 0.1867 Tier II
18 NC_000068.7 2 60369564 - Ly75 NNNCACTCACTAAAGAAATG NAG 2 0.1678 Tier II
19 NC_000076.6 10 39489766 - Fyn NNNCACTCACTAAAGAAATG NAG 2 0.1678 Tier II
20 NC_000068.7 2 155490569 + Ggt7 NNNTAGTCACTTAAGAAATG NGG 2 0.1667 Tier II
21 NC_000067.6 1 90791177 - Col6a3 NNNTACTCACTGAAGGAATG NGG 2 0.1389 Tier II
22 NC_000070.6 4 14931978 - Gm11837 NNNTACTCACTCAAGAAGTG NGG 2 0.1053 Tier II
23 NC_000074.6 8 14844542 + Dlgap2 NNNTGCTCACTAAATAAATG NGG 2 0.1029 Tier II
24 NC_000084.6 18 62460072 + Htr4 NNNTACTCACTTAACAAATG NGG 2 0.0909 Tier II
25 NC_000079.6 13 44819756 - Jarid2 NNNTACTCCCTAAATAAATG NGG 2 0.0816 Tier II
26 NC_000070.6 4 119557076 - Foxj3 NNNTACTCACTAAACAAATG NAG 2 0.0707 Tier II
27 NC_000086.7 X 144527623 - Trpc5 NNNTACTTACTAAAGAAATG NGA 2 0.0608 Tier II
28 NC_000071.6 5 20452610 + Magi2 NNNTACCCACTAAAGAAATG NGA 2 0.0477 Tier II
29 NC_000079.6 13 114879568 - Itga2 NNNTACTCACTAAATAAATG NAG 2 0.037 Tier II
30 NC_000070.6 4 139103213 + Micos10 NNNTACTCACTAAAGGACTG NGG 2 0.0366 Tier II
31 NC_000067.6 1 37325314 - Inpp4a NNNTCCTCACTAAAGAAATG NGA 2 0.0347 Tier II
32 NC_000072.6 6 38724190 - Hipk2 NNNTACTCACTTAAGAAATG NGA 2 0.0231 Tier II
33 NC_000079.6 13 109137315 + Pde4d NNNTACTCACTAAACAAATG NGA 2 0.0189 Tier II
34 NC_000072.6 6 86447439 - C87436 NNNTACACACTAAAGAAATG NGT 2 0.0141 Tier II
35 NC_000079.6 13 37828970 + Rreb1 NNNTACTCACTTAAGAAATG NTG 2 0.013 Tier II
36 NC_000076.6 10 77082554 - Col18a1 NNNTCCTCACTAAAGAAATG NGC 2 0.0111 Tier II
37 NC_000069.6 3 63950933 + Slc33a1 NNNTACTCACTAAAGAAAAG NGC 2 0.0063 Tier II
38 NC_000070.6 4 28948250 - Epha7 NNNTACTCACTAAAGCATTG NGG 2 0.0 Tier II
39 NC_000074.6 8 38237789 - Sgcz NNNTGCTCACTAAAGTAATG NGG 2 0.0 Tier II
40 NC_000075.6 9 29066697 + Gm15520 NNNTGCTCATTAAAGAAATG NGG 2 0.6776 Tier III
41 NC_000079.6 13 105248609 - Gm52064 NNNTACACACTATAGAAATG NGG 2 0.2625 Tier III
42 NC_000075.6 9 71204790 + Gm32511 NNNTTCTCACTTAAGAAATG NGG 2 0.1212 Tier III
43 NC_000067.6 1 120639245 + 2610027F03Rik NNNTACTCACTAAAGGACTG NGG 2 0.0366 Tier III
44 NC_000067.6 1 37325314 - 4930439A04Rik NNNTCCTCACTAAAGAAATG NGA 2 0.0347 Tier III
45 NC_000077.6 11 109029116 + Gm36876 NNNTACTCACAAAAGAAATG NGC 2 0.0167 Tier III
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Other clones with same target sequence:

(none)