Construct: sgRNA BRDN0001146547

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAAGGAAATGTGAGCCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TESK2 (10420)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76432

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 45457686 - TESK2 NNNAGGAAATGTGAGCCAGG NGG 0 1.0 Tier I
2 NC_000007.14 7 127123462 - GRM8 NNNAGGAAATGTGAGACAGA NGG 2 0.9375 Tier II
3 NC_000010.11 10 95468799 + SORBS1 NNNAAGAAATGTGAACCAGG NGG 2 0.8157 Tier II
4 NC_000003.12 3 57809712 - SLMAP NNNAGGAAATGTGAGCCAGT NGG 1 0.7 Tier II
5 NC_000003.12 3 37799760 + ITGA9 NNNACGAGATGTGAGCCAGG NGG 2 0.5762 Tier II
6 NC_000011.10 11 57377051 - PRG3 NNNAGCAAATGTGAGCCAGT NGG 2 0.4773 Tier II
7 NC_000005.10 5 6455833 - UBE2QL1 NNNAGGGAGTGTGAGCCAGG NGG 2 0.4706 Tier II
8 NC_000018.10 18 59617474 + CCBE1 NNNAGGAAATGTGAGAAAGG NGG 2 0.4667 Tier II
9 NC_000011.10 11 132123034 - NTM NNNAGGAAAAGTGTGCCAGG NGG 2 0.4571 Tier II
10 NC_000004.12 4 82701771 + SCD5 NNNAGGAAATATGAGCCACG NGG 2 0.4483 Tier II
11 NC_000006.12 6 108322407 + AFG1L NNNAGGAAAAGGGAGCCAGG NGG 2 0.4286 Tier II
12 NC_000002.12 2 217981752 - TNS1 NNNAGGATATGTGAGACAGG NGG 2 0.4286 Tier II
13 NC_000017.11 17 30795525 + CRLF3 NNNAGAAAATGTCAGCCAGG NGG 2 0.4211 Tier II
14 NC_000001.11 1 241709882 + WDR64 NNNAGGAAATTTGAGACAGG NGG 2 0.3846 Tier II
15 NC_000020.11 20 4168396 + SMOX NNNAGGCAAAGTGAGCCAGG NGG 2 0.375 Tier II
16 NC_000007.14 7 77258247 - CCDC146 NNNAGGGAATGGGAGCCAGG NGG 2 0.3529 Tier II
17 NC_000003.12 3 171650291 + PLD1 NNNAGGATATGAGAGCCAGG NGG 2 0.3429 Tier II
18 NC_000017.11 17 50458884 - ACSF2 NNNAGGAAATGGGAGCCATG NGG 2 0.3333 Tier II
19 NC_000009.12 9 114481249 + WHRN NNNAGGAAGGGTGAGCCAGG NGG 2 0.3333 Tier II
20 NC_000006.12 6 30926061 + VARS2 NNNAGGGAATGTGGGCCAGG NGG 2 0.3294 Tier II
21 NC_000010.11 10 113709635 + CASP7 NNNGGGAAATGTGAGCCTGG NGG 2 0.3125 Tier II
22 NC_000002.12 2 96281579 - SNRNP200 NNNAGGAAATTAGAGCCAGG NGG 2 0.3077 Tier II
23 NC_000017.11 17 80219847 + SGSH NNNCGGAAATGAGAGCCAGG NGG 2 0.2824 Tier II
24 NC_000006.12 6 150707907 - PLEKHG1 NNNAGGAAAGGGGAGCCAGG NGG 2 0.25 Tier II
25 NC_000001.11 1 182657101 - RGS8 NNNAGGAAAGGTGGGCCAGG NGG 2 0.2333 Tier II
26 NC_000023.11 X 6745941 - PUDP NNNAGGACATGGGAGCCAGG NGG 2 0.2143 Tier II
27 NC_000023.11 X 10457760 - MID1 NNNAGGATAGGTGAGCCAGG NGG 2 0.2143 Tier II
28 NC_000017.11 17 78238074 - TMEM235 NNNAGGGAATGTTAGCCAGG NGG 2 0.2118 Tier II
29 NC_000023.11 X 102248494 - NXF2 NNNAGGAAATGTCAGCCTGG NGG 2 0.2105 Tier II
30 NC_000023.11 X 102438618 + NXF2B NNNAGGAAATGTCAGCCTGG NGG 2 0.2105 Tier II
31 NC_000023.11 X 148803492 + AFF2 NNNAGGGAATGTGAGCCAGG NAG 2 0.183 Tier II
32 NC_000011.10 11 83525534 + DLG2 NNNAGGAAAGGTGAGCCAGG NAG 2 0.1296 Tier II
33 NC_000015.10 15 67461332 - IQCH NNNAGGAAATGAGATCCAGG NGG 2 0.1143 Tier II
34 NC_000023.11 X 105431969 + IL1RAPL2 NNNAGGAAATGAGATCCAGG NGG 2 0.1143 Tier II
35 NC_000010.11 10 48750579 - WDFY4 NNNAGGAAATGTGAGGCAGT NGG 2 0.1077 Tier II
36 NC_000001.11 1 218312736 + RRP15 NNNAGGAAATGTGACTCAGG NGG 2 0.0839 Tier II
37 NC_000019.10 19 16584463 + MED26 NNNAGGAAATGTGAGGAAGG NGG 2 0.0718 Tier II
38 NC_000022.11 22 41332509 - ZC3H7B NNNAGGAAATGTGGGGCAGG NGG 2 0.0718 Tier II
39 NC_000012.12 12 12153715 - LRP6 NNNAGGAAATGTGAGAGAGG NGG 2 0.0588 Tier II
40 NC_000004.12 4 25267337 + PI4K2B NNNAGGAGATGTGAGCCAGG NGA 2 0.0509 Tier II
41 NC_000007.14 7 122505689 + CADPS2 NNNAGGAAATGTGAGCCATG NGA 2 0.0463 Tier II
42 NC_000006.12 6 7794750 + BMP6 NNNAGGAAATGTGAGGCAGG NAG 2 0.0399 Tier II
43 NC_000011.10 11 8779214 + DENND2B NNNAGGAAATGTGAGCCAGA NTG 2 0.0365 Tier II
44 NC_000019.10 19 33723065 - CHST8 NNNTGGAAATGTGAGCCAGG NTG 2 0.0248 Tier II
45 NC_000020.11 20 36433166 + DLGAP4 NNNAGGAAATGTGAGCGAGG NAG 2 0.0153 Tier II
46 NC_000023.11 X 130064538 + ELF4 NNNTGGAAATGTGAGCCAGG NGC 2 0.0141 Tier II
47 NC_000006.12 6 38503700 - BTBD9 NNNAGGAAAAGTGAGCCAGG NGT 2 0.0138 Tier II
48 NC_000001.11 1 98708343 - SNX7 NNNAGGAAATGTCAGCCAGG NGC 2 0.0094 Tier II
49 NC_000004.12 4 89702424 + LOC124900602 NNNAGAGAATGTGAGCCAGG NGG 2 0.7059 Tier III
50 NC_000003.12 3 37799760 + ITGA9-AS1 NNNACGAGATGTGAGCCAGG NGG 2 0.5762 Tier III
51 NC_000007.14 7 103469914 - SLC26A5-AS1 NNNAGAAAATGTGAGCCTGG NGG 2 0.5 Tier III
52 NC_000005.10 5 6455833 - LOC105374639 NNNAGGGAGTGTGAGCCAGG NGG 2 0.4706 Tier III
53 NC_000002.12 2 217981752 - TNS1-AS1 NNNAGGATATGTGAGACAGG NGG 2 0.4286 Tier III
54 NC_000003.12 3 194023512 - LINC02028 NNNAGGAAATGTCAGACAGG NGG 2 0.4211 Tier III
55 NC_000022.11 22 47795797 - EPIC1 NNNAGGAGCTGTGAGCCAGG NGG 2 0.419 Tier III
56 NC_000008.11 8 30229594 - LOC124901928 NNNAGGAACTGTGAGCCAAG NGG 2 0.4082 Tier III
57 NC_000001.11 1 241709882 + LOC124904603 NNNAGGAAATTTGAGACAGG NGG 2 0.3846 Tier III
58 NC_000020.11 20 4168396 + LOC124904861 NNNAGGCAAAGTGAGCCAGG NGG 2 0.375 Tier III
59 NC_000007.14 7 77258247 - LOC102723791 NNNAGGGAATGGGAGCCAGG NGG 2 0.3529 Tier III
60 NC_000005.10 5 23160187 - LOC105374687 NNNAGTAAATGTGAGCTAGG NGG 2 0.3111 Tier III
61 NC_000008.11 8 33976318 + LOC105379364 NNNAGGAAATCTGAGCCAAG NGG 2 0.3061 Tier III
62 NC_000002.12 2 16054139 + GACAT3 NNNAGGATATGTGAGCCAGT NGG 2 0.3 Tier III
63 NC_000009.12 9 134212798 - LOC107987137 NNNAGGAAATGGGGGCCAGG NGG 2 0.2333 Tier III
64 NC_000012.12 12 126405364 - LINC02825 NNNAGGAAATCTGAGCAAGG NGG 2 0.2 Tier III
65 NC_000012.12 12 126405364 - LOC124903048 NNNAGGAAATCTGAGCAAGG NGG 2 0.2 Tier III
66 NC_000002.12 2 175622450 - LOC107985962 NNNAGGCCATGTGAGCCAGG NGG 2 0.1875 Tier III
67 NC_000012.12 12 92610927 + LOC105369905 NNNAGGAAATTTGAGTCAGG NGG 2 0.1183 Tier III
68 NC_000012.12 12 92610927 + LOC124902983 NNNAGGAAATTTGAGTCAGG NGG 2 0.1183 Tier III
69 NC_000015.10 15 67461332 - IQCH-AS1 NNNAGGAAATGAGATCCAGG NGG 2 0.1143 Tier III
70 NC_000011.10 11 8779214 + DENND2B-AS1 NNNAGGAAATGTGAGCCAGA NTG 2 0.0365 Tier III
71 NC_000010.11 10 120662760 + LINC02930 NNNAGAAAATGTGAGCCAGG NGT 2 0.0161 Tier III
72 NC_000019.10 19 56709108 - LOC105372472 NNNAGAAAATGTGAGCCAGG NGT 2 0.0161 Tier III
73 NC_000019.10 19 56709108 - LOC105372473 NNNAGAAAATGTGAGCCAGG NGT 2 0.0161 Tier III
74 NC_000022.11 22 31988728 + LINC02558 NNNAGGAAATGTGGGCCAGG NGT 2 0.0075 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 109011427 - Hmgn5 NNNAGGTGATGTGAGCCAGG NGG 2 0.3208 Tier I
2 NC_000071.6 5 112338311 - Srrd NNNAGGAAAGGTGAGCCAGC NGG 2 0.2143 Tier I
3 NC_000068.7 2 162230907 + Ptprt NNNAGGAAATGTGAAACAGG NGG 2 0.9412 Tier II
4 NC_000085.6 19 36418270 - Pcgf5 NNNAAGAAATGTGAGACAGG NGG 2 0.8667 Tier II
5 NC_000079.6 13 10033232 - Chrm3 NNNAGCAAATGTGAGACAGG NGG 2 0.6818 Tier II
6 NC_000074.6 8 94233045 + Nup93 NNNAGGAAGTGTGAGACAGG NGG 2 0.6667 Tier II
7 NC_000071.6 5 123995170 + Hip1r NNNAATAAATGTGAGCCAGG NGG 2 0.5778 Tier II
8 NC_000068.7 2 144398915 + Kat14 NNNAGGAATTGTGAACCAGG NGG 2 0.5647 Tier II
9 NC_000072.6 6 145229094 + Kras NNNTGGAAATGAGAGCCAGG NGG 2 0.5091 Tier II
10 NC_000079.6 13 57517724 + Spock1 NNNAGAAAATGGGAGCCAGG NGG 2 0.5 Tier II
11 NC_000067.6 1 154806447 + Cacna1e NNNAGACAATGTGAGCCAGG NGG 2 0.4375 Tier II
12 NC_000071.6 5 129004271 - Stx2 NNNAGAACATGTGAGCCAGG NGG 2 0.4286 Tier II
13 NC_000082.6 16 95360147 - Erg NNNGGGAAATGTGAGCCATG NGG 2 0.4167 Tier II
14 NC_000069.6 3 135377402 - Slc9b1 NNNAAGAAATCTGAGCCAGG NGG 2 0.3714 Tier II
15 NC_000068.7 2 126678564 + Gabpb1 NNNAGTAAACGTGAGCCAGG NGG 2 0.3556 Tier II
16 NC_000081.6 15 66184334 - Kcnq3 NNNAGGAAATGGGAGCCAGT NGG 2 0.35 Tier II
17 NC_000084.6 18 10584584 + Esco1 NNNAGGAAATCAGAGCCAGG NGG 2 0.3429 Tier II
18 NC_000070.6 4 97648466 + Nfia NNNAGGAAATGTGAGCAAAG NGG 2 0.3333 Tier II
19 NC_000076.6 10 77036617 - Slc19a1 NNNAGGAAGGGTGAGCCAGG NGG 2 0.3333 Tier II
20 NC_000079.6 13 55476361 + Dbn1 NNNAGGAAGTGGGAGCCAGG NGG 2 0.3333 Tier II
21 NC_000085.6 19 40981363 - Blnk NNNAGGAGATCTGAGCCAGG NGG 2 0.3143 Tier II
22 NC_000068.7 2 14737548 + Cacnb2 NNNATGAAATGTGAGACAGG NGG 2 0.3 Tier II
23 NC_000074.6 8 84966783 - Rnaseh2a NNNAGGAAATGTGAGTCAGA NGG 2 0.2885 Tier II
24 NC_000068.7 2 121796396 - Frmd5 NNNAGGAAATGTGACCCAGG NGG 1 0.2727 Tier II
25 NC_000075.6 9 70310625 - Myo1e NNNAGGAAACGTTAGCCAGG NGG 2 0.16 Tier II
26 NC_000084.6 18 72057265 + Dcc NNNAGGAAATGTAAGGCAGG NGG 2 0.142 Tier II
27 NC_000068.7 2 122777577 + Sqor NNNAGGAAATGTGACCCTGG NGG 2 0.1364 Tier II
28 NC_000077.6 11 99627519 + Krtap4-1 NNNATGTAATGTGAGCCAGG NGG 2 0.1313 Tier II
29 NC_000067.6 1 33896219 + Bend6 NNNAGGAAATGTCAGTCAGG NGG 2 0.1296 Tier II
30 NC_000074.6 8 48999680 - Tenm3 NNNAGGAAATCTTAGCCAGG NGG 2 0.1286 Tier II
31 NC_000070.6 4 149721099 + Tmem201 NNNAGGAAATGAGAGGCAGG NGG 2 0.1231 Tier II
32 NC_000069.6 3 27189893 + Nceh1 NNNTGGAAATGTGAGGCAGG NGG 2 0.0979 Tier II
33 NC_000081.6 15 76511254 - Dgat1 NNNAGGAAATGTGAGCCCCG NGG 2 0.0854 Tier II
34 NC_000068.7 2 9989540 + Taf3 NNNAGGAAATCTGAGGCAGG NGG 2 0.0659 Tier II
35 NC_000070.6 4 151643576 - Camta1 NNNAGGAAATCTGAGGCAGG NGG 2 0.0659 Tier II
36 NC_000083.6 17 70728530 + Dlgap1 NNNAGGAAATCTGAGGCAGG NGG 2 0.0659 Tier II
37 NC_000075.6 9 99042474 + Pik3cb NNNAGGAAATGTGAGGCAGC NGG 2 0.0659 Tier II
38 NC_000084.6 18 84065647 - Tshz1 NNNAGGAGATGTGAGCCAGG NGA 2 0.0509 Tier II
39 NC_000083.6 17 11278654 + Prkn NNNAGGAAATGTGAGCCATG NGA 2 0.0463 Tier II
40 NC_000082.6 16 17790483 - Klhl22 NNNAGGAAATGTTATCCAGG NGG 2 0.0429 Tier II
41 NC_000067.6 1 18115069 - Crisp4 NNNAGGAAATGAGAGCCAGG NTG 2 0.0312 Tier II
42 NC_000073.6 7 101768711 + Clpb NNNAGGAACTGTGAGCCAGG NTG 2 0.0223 Tier II
43 NC_000079.6 13 109231309 - Pde4d NNNATGAAATGTGAGCCAGG NGA 2 0.0208 Tier II
44 NC_000067.6 1 61653037 + Pard3b NNNAGGAAATGTGAGCTAGG NTG 2 0.0182 Tier II
45 NC_000068.7 2 32731115 - Sh2d3c NNNAGGAAATGAGAGCCAGG NGC 2 0.0178 Tier II
46 NC_000075.6 9 9944726 - Cntn5 NNNAGGAGATGTGAGCCAGG NGC 2 0.0163 Tier II
47 NC_000074.6 8 114205528 + Vat1l NNNAGGAAATGTGAGGGAGG NGG 2 0.009 Tier II
48 NC_000069.6 3 108473350 + Elapor1 NNNAGGAAATGTGTGCCAGG NGT 2 0.0086 Tier II
49 NC_000080.6 14 9825111 - Fhit NNNAGGAAATGTGGGCCAGG NGT 2 0.0075 Tier II
50 NC_000073.6 7 65900351 - Pcsk6 NNNATGAAATGTGAGCCAGG NGC 2 0.0067 Tier II
51 NC_000069.6 3 30678705 - Lrrc31 NNNAGGAAATTTGAGCCAGG NGT 2 0.0062 Tier II
52 NC_000075.6 9 36920545 - Pknox2 NNNAGGAAATGTGAGGCAGG NGT 2 0.0025 Tier II
53 NC_000069.6 3 103075661 + Ampd1 NNNAGGAAATGTGAGCCAGG NCA 2 0.0 Tier II
54 NC_000078.6 12 66956041 + Mdga2 NNNAGGAAATGTGAGCCAGG NTA 2 0.0 Tier II
55 NC_000070.6 4 131631271 + Gm42320 NNNAGGAAATGTGAGCCTGG NGG 1 0.5 Tier III
56 NC_000082.6 16 29968879 - Gm1968 NNNAGGAAATGTCAGACAGG NGG 2 0.4211 Tier III
57 NC_000068.7 2 179040937 - Gm35948 NNNAAGTAATGTGAGCCAGG NGG 2 0.3792 Tier III
58 NC_000082.6 16 50030185 - Gm4827 NNNATGAGATGTGAGCCAGG NGG 2 0.22 Tier III
59 NC_000067.6 1 21336972 + Gm21909 NNNAGGACATGTGAGTCAGG NGG 2 0.1319 Tier III
60 NC_000077.6 11 99627519 + Gm32730 NNNATGTAATGTGAGCCAGG NGG 2 0.1313 Tier III
61 NC_000084.6 18 28918129 + 4930474G06Rik NNNAGGAAATGAGAGGCAGG NGG 2 0.1231 Tier III
62 NC_000084.6 18 28918129 + Gm33948 NNNAGGAAATGAGAGGCAGG NGG 2 0.1231 Tier III
63 NC_000070.6 4 151643576 - Gm38442 NNNAGGAAATCTGAGGCAGG NGG 2 0.0659 Tier III
64 NC_000078.6 12 71661400 + 9630002D21Rik NNNAGGAAATGTGAGCCTGG NGA 2 0.0347 Tier III
65 NC_000076.6 10 47084251 + Gm3442 NNNAGGAAATGTGAGTCAGG NGA 2 0.0214 Tier III
66 NC_000074.6 8 114205528 + Gm30272 NNNAGGAAATGTGAGGGAGG NGG 2 0.009 Tier III
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Other clones with same target sequence:

(none)