Construct: sgRNA BRDN0001146550

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CGTCAGGCAGATCCAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA8 (2046)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76527

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 22595257 - EPHA8 NNNCAGGCAGATCCAGACAA NGG 0 1.0 Tier I
2 NC_000009.12 9 34622505 + ARID3C NNNCAGGCAGTGCCAGACAA NGG 2 0.1538 Tier I
3 NC_000011.10 11 75730250 + MOGAT2 NNNCAGGCAGAACCAGAGAA NGG 2 0.1067 Tier I
4 NC_000003.12 3 123101823 - PDIA5 NNNCAGACAGGTCCAGACAA NGG 2 0.65 Tier II
5 NC_000010.11 10 71368509 - SLC29A3 NNNCAGGGAGATCCAGACAG NGG 2 0.4916 Tier II
6 NC_000007.14 7 105975737 - CDHR3 NNNTAGGCAGATCCAGACAT NGG 2 0.48 Tier II
7 NC_000005.10 5 128424156 + FBN2 NNNCAGGCAGCTCCAGACAA NGG 1 0.4 Tier II
8 NC_000003.12 3 195142338 + XXYLT1 NNNCAGGCAGATCTAGAAAA NGG 2 0.3949 Tier II
9 NC_000011.10 11 125225138 - PKNOX2 NNNCAGGCACATCCAGACAG NGG 2 0.3059 Tier II
10 NC_000003.12 3 134713701 + CEP63 NNNCAGTCAGAGCCAGACAA NGG 2 0.2857 Tier II
11 NC_000017.11 17 68522254 + PRKAR1A NNNCTGGCAGATCCAGAAAA NGG 2 0.1958 Tier II
12 NC_000011.10 11 125927284 - DDX25 NNNCAGGCAGTTCCAGACAT NGG 2 0.1846 Tier II
13 NC_000006.12 6 25399298 - CARMIL1 NNNCTGGCAGAGCCAGACAA NGG 2 0.1818 Tier II
14 NC_000011.10 11 44819903 + TSPAN18 NNNCAGACAGATCCAGGCAA NGG 2 0.1765 Tier II
15 NC_000022.11 22 44028518 - PARVB NNNCTGGCACATCCAGACAA NGG 2 0.1455 Tier II
16 NC_000020.11 20 34016785 - RALY NNNCAGGCAGACCCAGAGAA NGG 2 0.1263 Tier II
17 NC_000006.12 6 166645640 - RPS6KA2 NNNCAGGTAGATCCAGAGAA NGG 2 0.1167 Tier II
18 NC_000015.10 15 27866152 + OCA2 NNNAAGGCAGATCCAGAGAA NGG 2 0.1123 Tier II
19 NC_000006.12 6 130995750 - EPB41L2 NNNCAGGCAGATCATGACAA NGG 2 0.07 Tier II
20 NC_000002.12 2 161920028 - SLC4A10 NNNCAGGCAGTTCCAGACAC NGG 2 0.0699 Tier II
21 NC_000006.12 6 69973278 - COL19A1 NNNCAGGCAGATCCTGAGAA NGG 2 0.0267 Tier II
22 NC_000019.10 19 6819576 - VAV1 NNNCAGGCAAATCCACACAA NGG 2 0.0 Tier II
23 NC_000013.11 13 66308577 + PCDH9 NNNCAGGCAGAGCCATACAA NGG 2 0.0 Tier II
24 NC_000002.12 2 25246567 - DNMT3A NNNCAGGCAGATCCACACCA NGG 2 0.0 Tier II
25 NC_000003.12 3 140259841 - CLSTN2 NNNCAGGCATATCCATACAA NGG 2 0.0 Tier II
26 NC_000003.12 3 130752883 + LOC107986023 NNNCAGACAGATCCAGAAAA NGG 2 0.5385 Tier III
27 NC_000008.11 8 85855147 + LINC02849 NNNCAGCCAGATCTAGACAA NGG 2 0.5042 Tier III
28 NC_000012.12 12 53785133 - LOC105378250 NNNCAGGCAGAGCCAAACAA NGG 2 0.5 Tier III
29 NC_000018.10 18 24979319 + LINC01894 NNNCAGGAAGATCCAGACAG NGG 2 0.4971 Tier III
30 NC_000006.12 6 2261664 - GMDS-DT NNNCTGGTAGATCCAGACAA NGG 2 0.3182 Tier III
31 NC_000014.9 14 76238627 - LOC105370575 NNNCAGACAGATCCAGACAC NGG 2 0.2273 Tier III
32 NC_000011.10 11 125927284 - DDX25-AS1 NNNCAGGCAGTTCCAGACAT NGG 2 0.1846 Tier III
33 NC_000006.12 6 25399298 - LOC124901281 NNNCTGGCAGAGCCAGACAA NGG 2 0.1818 Tier III
34 NC_000006.12 6 7443695 - LOC102724234 NNNCAGACAGATGCAGACAA NGG 2 0.1364 Tier III
35 NC_000011.10 11 22152285 + LOC124902645 NNNCAGACAGATCCAGAGAA NGG 2 0.1333 Tier III
36 NC_000006.12 6 166645640 - LOC107986671 NNNCAGGTAGATCCAGAGAA NGG 2 0.1167 Tier III
37 NC_000008.11 8 76629334 + ZFHX4-AS1 NNNCAGGGAGATCCAGTCAA NGG 2 0.0857 Tier III
38 NC_000013.11 13 66308577 + PCDH9-AS1 NNNCAGGCAGAGCCATACAA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 136935118 + Epha8 NNNCAGGCAGATCCAGACGA NGG 1 0.375 Tier I
2 NC_000073.6 7 80232397 + Cib1 NNNAAGGCAAATCCAGACAA NGG 2 0.786 Tier II
3 NC_000076.6 10 82673731 - Glt8d2 NNNCAGGGAAATCCAGACAA NGG 2 0.6 Tier II
4 NC_000071.6 5 145456986 - Cyp3a16 NNNAAGGCAGATCCAGATAA NGG 2 0.5414 Tier II
5 NC_000085.6 19 56908803 + Vwa2 NNNCAGCCAGATCTAGACAA NGG 2 0.5042 Tier II
6 NC_000071.6 5 122983500 + Kdm2b NNNCAGACAGAGCCAGACAA NGG 2 0.5 Tier II
7 NC_000084.6 18 60941123 - Camk2a NNNCAGGAAGATCCAGACAG NGG 2 0.4971 Tier II
8 NC_000067.6 1 152572259 + Rgl1 NNNCAGGCTGATCTAGACAA NGG 2 0.44 Tier II
9 NC_000071.6 5 30712165 + Dpysl5 NNNCAGGCAGATCCGGACTA NGG 2 0.35 Tier II
10 NC_000084.6 18 35657122 - Spata24 NNNCAGGGAGAGCCAGACAA NGG 2 0.3214 Tier II
11 NC_000067.6 1 63242038 + Zdbf2 NNNCAGGCAGTTCCAAACAA NGG 2 0.3077 Tier II
12 NC_000082.6 16 37378627 - Stxbp5l NNNCAGGCAGATATAGACAA NGG 2 0.2821 Tier II
13 NC_000068.7 2 113399286 + Fmn1 NNNCAGGCAGTTCCAGACAG NGG 2 0.2353 Tier II
14 NC_000069.6 3 90501827 + Chtop NNNCAGGCAGATACAGAAAA NGG 2 0.2071 Tier II
15 NC_000071.6 5 134445536 + Gtf2ird1 NNNCAGGCAGAGACAGACAA NGG 2 0.1923 Tier II
16 NC_000076.6 10 81149310 + Zbtb7a NNNCAGGCAGAGACAGACAA NGG 2 0.1923 Tier II
17 NC_000070.6 4 128800424 + Zfp362 NNNCAAGCAGATCCAGGCAA NGG 2 0.1765 Tier II
18 NC_000070.6 4 130572824 + Nkain1 NNNAAGGCAGATCCAGACCA NGG 2 0.1742 Tier II
19 NC_000084.6 18 82961374 - Zfp516 NNNCAGGCAGATCCAGAAAA NAG 2 0.1396 Tier II
20 NC_000079.6 13 11855885 + Ryr2 NNNCAGACAGATGCAGACAA NGG 2 0.1364 Tier II
21 NC_000070.6 4 99715980 - Alg6 NNNCAGGCACATCCCGACAA NGG 2 0.1091 Tier II
22 NC_000079.6 13 68875927 + Adcy2 NNNCAGGCAGATCCAGGAAA NGG 2 0.095 Tier II
23 NC_000082.6 16 26012032 + P3h2 NNNCAGGCAGATACAGACAC NGG 2 0.0874 Tier II
24 NC_000074.6 8 17153674 + Csmd1 NNNCAGGCAGCTCCAGCCAA NGG 2 0.0706 Tier II
25 NC_000084.6 18 37850218 + Diaph1 NNNCAGACAGATCCAGACAA NGA 2 0.0694 Tier II
26 NC_000083.6 17 88013236 + Fbxo11 NNNCAGGCAGATCCAGACAC NAG 2 0.0589 Tier II
27 NC_000078.6 12 75177147 + Kcnh5 NNNCAGGAAGATCCAGACAA NTG 2 0.0253 Tier II
28 NC_000067.6 1 11200078 - Prex2 NNNCAGGCAGATGCAGGCAA NGG 2 0.0241 Tier II
29 NC_000080.6 14 64807139 - Kif13b NNNCTGGCAGATCCAGACAA NGT 2 0.0059 Tier II
30 NC_000085.6 19 53228993 + Add3 NNNCAGGCAGATCCAGAGAA NTG 2 0.0052 Tier II
31 NC_000070.6 4 140075565 - Igsf21 NNNCAGCCAGATCCACACAA NGG 2 0.0 Tier II
32 NC_000084.6 18 25715254 + Celf4 NNNCAGGCAGATCCACACCA NGG 2 0.0 Tier II
33 NC_000077.6 11 61716602 + Slc5a10 NNNCAGGCATATCCACACAA NGG 2 0.0 Tier II
34 NC_000069.6 3 52549958 + Gm30173 NNNCAGGAAGATCCAAACAA NGG 2 0.65 Tier III
35 NC_000084.6 18 8416282 - Gm5500 NNNCAGGCAGGCCCAGACAA NGG 2 0.6158 Tier III
36 NC_000073.6 7 117258694 + Gm39075 NNNCTGGCAGATCCAGACAG NGG 2 0.2781 Tier III
37 NC_000074.6 8 116252553 - Gm31278 NNNCAGGCAGCTCCAGGCAA NGG 2 0.0706 Tier III
38 NC_000068.7 2 95446658 - Gm13794 NNNCAGGCAGATCCAGAAAA NGA 2 0.0374 Tier III
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Other clones with same target sequence:

(none)