Construct: sgRNA BRDN0001146560
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTCCCATGGTATCGTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP4K2 (5871)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76780
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64797365 |
+ |
MAP4K2 |
NNNTCCCATGGTATCGTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
218271121 |
+ |
PNKD |
NNNTCCCATGCTGTCGTCTG |
NGG |
2 |
0.2795 |
Tier II |
| 3 |
NC_000002.12 |
2 |
28594976 |
- |
PLB1 |
NNNTCCCATGCTATCGTATG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000008.11 |
8 |
18807486 |
- |
PSD3 |
NNNTCCCATGGTATCATCTG |
NGA |
2 |
0.0694 |
Tier II |
| 5 |
NC_000016.10 |
16 |
19297410 |
- |
CLEC19A |
NNNTCCCATGGTAACGTCTG |
NGA |
2 |
0.043 |
Tier II |
| 6 |
NC_000005.10 |
5 |
67020917 |
+ |
MAST4 |
NNNTCCCATGGGATGGTCTG |
NGG |
2 |
0.025 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
126744907 |
+ |
Camk2d |
NNNTCCCCTGGTATGGTCTG |
NGG |
2 |
0.0214 |
Tier II |
| 2 |
NC_000070.6 |
4 |
125713813 |
- |
Grik3 |
NNNTCCCATGGTACTGTCTG |
NGG |
2 |
0.019 |
Tier II |
| 3 |
NC_000068.7 |
2 |
44394549 |
- |
Arhgap15 |
NNNTCCCATGGCATCTTCTG |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000070.6 |
4 |
35886990 |
+ |
Lingo2 |
NNNTCCCATGGTACCTTCTG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000072.6 |
6 |
48896558 |
+ |
Aoc1 |
NNNTCCCATGGTATCTTTTG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000069.6 |
3 |
68541082 |
+ |
Schip1 |
NNNTCCCTTGGTATCCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000069.6 |
3 |
68541082 |
+ |
Iqschfp |
NNNTCCCTTGGTATCCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000068.7 |
2 |
38693557 |
+ |
Nr5a1 |
NNNTCTCATGGTATCCTCTG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000072.6 |
6 |
48896558 |
+ |
Gm44764 |
NNNTCCCATGGTATCTTTTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
