Construct: sgRNA BRDN0001146568

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTATCCTCAAAGTATGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK3 (4752)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77357

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 52144850 - NEK3 NNNTCCTCAAAGTATGTCAA NGG 0 1.0 Tier I
2 NC_000009.12 9 36582711 - MELK NNNTCCTCAAAATATATCAA NGG 2 0.9333 Tier II
3 NC_000012.12 12 13056329 - FAM234B NNNTCATCAAAGAATGTCAA NGG 2 0.6429 Tier II
4 NC_000005.10 5 66930668 - MAST4 NNNTCCTGAAAGCATGTCAA NGG 2 0.5075 Tier II
5 NC_000011.10 11 73536345 + FAM168A NNNTCATCAAAGTTTGTCAA NGG 2 0.4952 Tier II
6 NC_000015.10 15 71696690 - THSD4 NNNTCCTCAAGGTATGCCAA NGG 2 0.4588 Tier II
7 NC_000014.9 14 90750328 + TTC7B NNNTCCCCAGAGTATGTCAA NGG 2 0.3819 Tier II
8 NC_000003.12 3 77453037 + ROBO2 NNNTCCCCAAAGTATGTCTA NGG 2 0.3702 Tier II
9 NC_000008.11 8 29001145 + HMBOX1 NNNTCCTCAAAGTATGAAAA NGG 2 0.2872 Tier II
10 NC_000001.11 1 12313773 - VPS13D NNNTCCCCAAAGGATGTCAA NGG 2 0.1793 Tier II
11 NC_000017.11 17 57607106 - MSI2 NNNTCCTCAAAGAATGTCCA NGG 2 0.1432 Tier II
12 NC_000005.10 5 142324838 - SPRY4 NNNTCCTCAAAGTAAGTCCA NGG 2 0.1198 Tier II
13 NC_000006.12 6 101453917 - GRIK2 NNNTCCTCAAAGTCTGTCAC NGG 2 0.0487 Tier II
14 NC_000004.12 4 140084785 - MAML3 NNNTCCTCAAAGTAGGTTAA NGG 2 0.0321 Tier II
15 NC_000005.10 5 111587782 + STARD4-AS1 NNNTCCTCAAAGAATATCAA NGG 2 0.6923 Tier III
16 NC_000006.12 6 95960767 + LOC107986626 NNNACCTTAAAGTATGTCAA NGG 2 0.4167 Tier III
17 NC_000017.11 17 57607106 - LOC101927539 NNNTCCTCAAAGAATGTCCA NGG 2 0.1432 Tier III
18 NC_000005.10 5 111556076 - STARD4-AS1 NNNTCCTCAAAATATGTCAA NGA 2 0.0648 Tier III
19 NC_000006.12 6 101453917 - LOC101927388 NNNTCCTCAAAGTCTGTCAC NGG 2 0.0487 Tier III
20 NC_000020.11 20 22603198 - LNCNEF NNNACCTCAAAGTAGGTCAA NGG 2 0.0238 Tier III
21 NC_000006.12 6 113525947 - LOC124901379 NNNTCCTCAAATTATGTCAA NGT 2 0.0062 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 145362471 + Slc24a3 NNNTCTTCAAAGAATGTCAA NGG 2 0.6429 Tier II
2 NC_000084.6 18 60979188 + Camk2a NNNTCCTCCAAGTATGTCAA NGG 1 0.5714 Tier II
3 NC_000080.6 14 75463892 + Siah3 NNNTACTCAAAGTATGCCAA NGG 2 0.4034 Tier II
4 NC_000069.6 3 152195709 - Dnajb4 NNNTCCTCACAGTATATCAA NGG 2 0.3333 Tier II
5 NC_000076.6 10 106557632 - Ppfia2 NNNACCTCAAAGTATGTAAA NGG 2 0.2564 Tier II
6 NC_000074.6 8 87798584 - Zfp423 NNNTCCTCAATGTATGTCAT NGG 2 0.1846 Tier II
7 NC_000073.6 7 49129787 - Nav2 NNNTCCTCAAAGTCTGCCAA NGG 2 0.1513 Tier II
8 NC_000075.6 9 28156511 - Opcml NNNTCCTCAAAATATGTCAA NGA 2 0.0648 Tier II
9 NC_000067.6 1 176218999 - Pld5 NNNTCCTCAAAGTATGTCAT NGA 2 0.0417 Tier II
10 NC_000078.6 12 81964896 + Pcnx NNNTCCTCAAAGTATGTCAT NTG 2 0.0234 Tier II
11 NC_000074.6 8 122524473 - Piezo1 NNNTCCTCAAAGTATGACAA NGT 2 0.0086 Tier II
12 NC_000067.6 1 86110016 - Htr2b NNNTCCTCAAAGTATCTCAA NAG 2 0.0 Tier II
13 NC_000067.6 1 86110016 - Psmd1 NNNTCCTCAAAGTATCTCAA NAG 2 0.0 Tier II
14 NC_000076.6 10 106557632 - 4930532I03Rik NNNACCTCAAAGTATGTAAA NGG 2 0.2564 Tier III
15 NC_000073.6 7 49129787 - 9130015G15Rik NNNTCCTCAAAGTCTGCCAA NGG 2 0.1513 Tier III
16 NC_000069.6 3 52943784 + Gm30351 NNNACCTCAAAGTATGTCAA NAG 2 0.1235 Tier III
17 NC_000084.6 18 26002769 + Gm33228 NNNTCCTCAAAGGCTGTCAA NGG 2 0.0559 Tier III
18 NC_000069.6 3 67543440 + Gm35299 NNNTCCCCAAAGTAGGTCAA NGG 2 0.0344 Tier III
19 NC_000079.6 13 78558442 + Gm32067 NNNTCCTCACAGTATCTCAA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)