GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001146569

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: GAACTACCCCAGCAAAAATG

Original Target:

Taxon:: Homo sapiens (human)
Gene:: CDKL3 (51265)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 75605

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000005.10	5	134319452	+	CDKL3	NNNCTACCCCAGCAAAAATG	NGG	0	1.0	Tier I
2	NC_000005.10	5	20227296	-	CDH18	NNNATATCCCAGCAAAAATG	NGG	2	0.6842	Tier II
3	NC_000024.10	Y	5533311	+	PCDH11Y	NNNCTACCACAGTAAAAATG	NGG	2	0.6	Tier II
4	NC_000002.12	2	233629346	-	UGT1A8	NNNCAACCCAAGCAAAAATG	NGG	2	0.4333	Tier II
5	NC_000020.11	20	15200387	-	MACROD2	NNNTTACCCCACCAAAAATG	NGG	2	0.4235	Tier II
6	NC_000007.14	7	133062615	+	CHCHD3	NNNCTACAGCAGCAAAAATG	NGG	2	0.4024	Tier II
7	NC_000010.11	10	23702128	+	KIAA1217	NNNCTACAGCAGCAAAAATG	NGG	2	0.4024	Tier II
8	NC_000008.11	8	84187861	+	RALYL	NNNCTACCCCAGTAAAAGTG	NGG	2	0.28	Tier II
9	NC_000001.11	1	113570726	-	MAGI3	NNNCTACCCCATCAAAAATT	NGG	2	0.2692	Tier II
10	NC_000004.12	4	113938748	+	ARSJ	NNNCTACCCCATTAAAAATG	NGG	2	0.2692	Tier II
11	NC_000020.11	20	58861105	+	GNAS	NNNCAACCCCAGCAAAATTG	NGG	2	0.25	Tier II
12	NC_000002.12	2	9618992	+	YWHAQ	NNNCTACCACAGCAAAAAAG	NGG	2	0.2449	Tier II
13	NC_000003.12	3	17393194	-	TBC1D5	NNNCAACCCCATCAAAAATG	NGG	2	0.1923	Tier II
14	NC_000023.11	X	114792952	-	HTR2C	NNNCAACCCCATCAAAAATG	NGG	2	0.1923	Tier II
15	NC_000003.12	3	155102731	+	MME	NNNCTACCTCAGCAAGAATG	NGG	2	0.1683	Tier II
16	NC_000001.11	1	216657830	+	ESRRG	NNNCTACCCCATAAAAAATG	NGG	2	0.1479	Tier II
17	NC_000018.10	18	80170898	-	PARD6G	NNNCTTCCCCAGCAAAACTG	NGG	2	0.1361	Tier II
18	NC_000011.10	11	67180558	+	KDM2A	NNNCTACCCCACCAAAACTG	NGG	2	0.1008	Tier II
19	NC_000006.12	6	56898151	-	DST	NNNCTACCCCAGCAAAAAAG	NAG	2	0.0741	Tier II
20	NC_000009.12	9	85559210	+	AGTPBP1	NNNCTACCCCAGGAAAAATG	NAG	2	0.0354	Tier II
21	NC_000010.11	10	62818470	+	EGR2	NNNCCACCCCAGCAAAAATG	NGC	2	0.0222	Tier II
22	NC_000002.12	2	233610585	-	UGT1A	NNNCAACCCAAGCAAAAATG	NGG	2	0.4333	Tier III
23	NC_000002.12	2	233629346	-	UGT1A	NNNCAACCCAAGCAAAAATG	NGG	2	0.4333	Tier III
24	NC_000016.10	16	32412621	-	SLC9B1P5	NNNCTACCCCATCAAAAATT	NGG	2	0.2692	Tier III
25	NT_187383.1	16	417014	+	LOC102723945	NNNCTACCCCATCAAAAATT	NGG	2	0.2692	Tier III
26	NC_000023.11	X	114792952	-	LOC105373313	NNNCAACCCCATCAAAAATG	NGG	2	0.1923	Tier III
27	NC_000016.10	16	14325556	+	MIR193BHG	NNNCTACCCCAGCAAGAATG	NGG	1	0.1923	Tier III
28	NC_000010.11	10	11657862	+	LOC105376413	NNNCTACGCCAGCAAAAAAG	NGG	2	0.1837	Tier III
29	NC_000023.11	X	57824786	-	LOC124905232	NNNCTACCCCAGCAAAAAGT	NGG	2	0.175	Tier III
30	NC_000018.10	18	80170898	-	PARD6G-AS1	NNNCTTCCCCAGCAAAACTG	NGG	2	0.1361	Tier III
31	NC_000005.10	5	151964718	-	LINC01933	NNNCTACCCCAGCAAAACTG	NAG	2	0.0494	Tier III
32	NC_000006.12	6	105846432	+	LOC105377923	NNNCTACCCCAGCAAAACTG	NAG	2	0.0494	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000068.7	2	35803051	+	Ttll11	NNNCTACGCCAGCAAAAATG	NGG	1	0.6429	Tier II
2	NC_000070.6	4	136725368	+	Ephb2	NNNATACCCCACCAAAAATG	NGG	2	0.4458	Tier II
3	NC_000084.6	18	64609652	-	Atp8b1	NNNATACCCCAGAAAAAATG	NGG	2	0.3239	Tier II
4	NC_000086.7	X	10170465	-	Rpgr	NNNCTACCCAAGCAAAAAAG	NGG	2	0.2476	Tier II
5	NC_000068.7	2	135286125	-	Plcb1	NNNCTTCCCCAGCACAAATG	NGG	2	0.1948	Tier II
6	NC_000081.6	15	80356655	-	Cacna1i	NNNCTACCCCAGGAAAAATG	NGG	1	0.1364	Tier II
7	NC_000077.6	11	117315355	-	Septin9	NNNCAACCCCAGGAAAAATG	NGG	2	0.0682	Tier II
8	NC_000070.6	4	140428453	+	Gm13021	NNNCTACCTCAGCAAACATG	NGG	2	0.1544	Tier III
9	NC_000077.6	11	113057549	+	2610035D17Rik	NNNCTACCCCTGCAAAAATG	NAG	2	0.0798	Tier III
10	NC_000077.6	11	20147964	+	Gm12031	NNNCTACCCCAGCAAGAATG	NAG	2	0.0499	Tier III

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Other clones with same target sequence:

(none)