Construct: sgRNA BRDN0001146573
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGAATAAACAAAAGTGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32A (202374)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76473
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
147279314 |
+ |
STK32A |
NNNGAATAAACAAAAGTGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
156038384 |
+ |
UBQLN4 |
NNNAAAAAAACAAAAGTGCG |
NGG |
2 |
0.7875 |
Tier II |
| 3 |
NC_000014.9 |
14 |
104964591 |
+ |
AHNAK2 |
NNNGAATAAAAAAAAATGCG |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000004.12 |
4 |
5737312 |
+ |
EVC |
NNNGAACAAACAAAAGTGCA |
NGG |
2 |
0.6445 |
Tier II |
| 5 |
NC_000005.10 |
5 |
78404886 |
+ |
SCAMP1 |
NNNGATAAAACAAAAGTGCG |
NGG |
2 |
0.625 |
Tier II |
| 6 |
NC_000009.12 |
9 |
123061094 |
- |
RABGAP1 |
NNNGAATAAACAAAAGTGAG |
NGG |
1 |
0.4286 |
Tier II |
| 7 |
NC_000011.10 |
11 |
126127631 |
+ |
LOC105369591 |
NNNGAAGAAACAAAAGTGAG |
NGG |
2 |
0.2521 |
Tier II |
| 8 |
NC_000018.10 |
18 |
5157353 |
- |
AKAIN1 |
NNNGCATAAACAAAAGTGAG |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000001.11 |
1 |
171733603 |
+ |
VAMP4 |
NNNGAATAAACACAAGTGCT |
NGG |
2 |
0.1474 |
Tier II |
| 10 |
NC_000018.10 |
18 |
53129287 |
- |
DCC |
NNNGAAGAAAGAAAAGTGCG |
NGG |
2 |
0.1471 |
Tier II |
| 11 |
NC_000008.11 |
8 |
116767358 |
- |
UTP23 |
NNNGAATAAACAAATGTGCT |
NGG |
2 |
0.14 |
Tier II |
| 12 |
NC_000018.10 |
18 |
52699035 |
+ |
DCC |
NNNGAATAAACAAATGTGCT |
NGG |
2 |
0.14 |
Tier II |
| 13 |
NC_000006.12 |
6 |
128210157 |
+ |
PTPRK |
NNNGAATAAAGAAAAGTGTG |
NGG |
2 |
0.1154 |
Tier II |
| 14 |
NC_000006.12 |
6 |
13470687 |
- |
GFOD1 |
NNNGAATAATCAAAAGTGGG |
NGG |
2 |
0.1103 |
Tier II |
| 15 |
NC_000008.11 |
8 |
84766902 |
+ |
RALYL |
NNNGCATAAACAACAGTGCG |
NGG |
2 |
0.1071 |
Tier II |
| 16 |
NC_000008.11 |
8 |
37826124 |
+ |
ADGRA2 |
NNNGAATAAACACAAGTGAG |
NGG |
2 |
0.0902 |
Tier II |
| 17 |
NC_000015.10 |
15 |
64765594 |
+ |
RBPMS2 |
NNNGAATAAAAAAAAGGGCG |
NGG |
2 |
0.0882 |
Tier II |
| 18 |
NC_000003.12 |
3 |
33619561 |
+ |
CLASP2 |
NNNGAAGAAACAAAAGTGGG |
NGG |
2 |
0.0735 |
Tier II |
| 19 |
NC_000002.12 |
2 |
205194214 |
+ |
PARD3B |
NNNGAATAATCAAAAGTGCG |
NGC |
2 |
0.0196 |
Tier II |
| 20 |
NC_000021.9 |
21 |
32924852 |
+ |
LOC105377136 |
NNNGAACAAACAAAAGTGCT |
NGG |
2 |
0.4812 |
Tier III |
| 21 |
NC_000011.10 |
11 |
126127631 |
+ |
LOC124902784 |
NNNGAAGAAACAAAAGTGAG |
NGG |
2 |
0.2521 |
Tier III |
| 22 |
NC_000005.10 |
5 |
85815739 |
- |
LOC105379062 |
NNNGAATAAACAAAACTGCC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
109004396 |
- |
Pde4d |
NNNGAAGAAACAAAAGTGCA |
NGG |
2 |
0.5515 |
Tier II |
| 2 |
NC_000085.6 |
19 |
8851298 |
- |
Lrrn4cl |
NNNGGATAAACAAAAGTGCT |
NGG |
2 |
0.504 |
Tier II |
| 3 |
NC_000080.6 |
14 |
33004200 |
+ |
Lrrc18 |
NNNGAATAAACAAAGGTGCT |
NGG |
2 |
0.455 |
Tier II |
| 4 |
NC_000080.6 |
14 |
33004200 |
+ |
Wdfy4 |
NNNGAATAAACAAAGGTGCT |
NGG |
2 |
0.455 |
Tier II |
| 5 |
NC_000068.7 |
2 |
101751264 |
+ |
Prr5l |
NNNAAATAAACAAAAGTGTG |
NGG |
2 |
0.4154 |
Tier II |
| 6 |
NC_000072.6 |
6 |
52633116 |
+ |
Hibadh |
NNNTAATAAACAAAAGTGCA |
NGG |
2 |
0.3409 |
Tier II |
| 7 |
NC_000086.7 |
X |
165296477 |
- |
Glra2 |
NNNGAACAAACAAAAGTGTG |
NGG |
2 |
0.3173 |
Tier II |
| 8 |
NC_000067.6 |
1 |
180160134 |
- |
Cdc42bpa |
NNNGAATAAACAAAAGTACC |
NGG |
2 |
0.2967 |
Tier II |
| 9 |
NC_000077.6 |
11 |
5242442 |
+ |
Kremen1 |
NNNGTATAAACAAAAGTGCT |
NGG |
2 |
0.2545 |
Tier II |
| 10 |
NC_000075.6 |
9 |
78585341 |
+ |
Slc17a5 |
NNNGAATAAACAAAAGTCTG |
NGG |
2 |
0.2198 |
Tier II |
| 11 |
NC_000076.6 |
10 |
76836813 |
- |
Pcbp3 |
NNNGAAGAAACCAAAGTGCG |
NGG |
2 |
0.1548 |
Tier II |
| 12 |
NC_000082.6 |
16 |
40903526 |
+ |
Lsamp |
NNNGCATAAAGAAAAGTGCG |
NGG |
2 |
0.125 |
Tier II |
| 13 |
NC_000067.6 |
1 |
24706149 |
+ |
Lmbrd1 |
NNNAAATAAACAAAAGTGGG |
NGG |
2 |
0.1125 |
Tier II |
| 14 |
NC_000077.6 |
11 |
93872637 |
- |
Utp18 |
NNNGAATAAACAAAAGGGCG |
NGA |
2 |
0.0082 |
Tier II |
| 15 |
NC_000077.6 |
11 |
36472713 |
- |
Tenm2 |
NNNGAATAAACAAAATTCCG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000073.6 |
7 |
83372505 |
- |
Gm29976 |
NNNCAATAAACAAAAGTGCA |
NGG |
2 |
0.4963 |
Tier III |
Other clones with same target sequence:
(none)
