Construct: sgRNA BRDN0001146574
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGGGTGCCATTGACCGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKL (5211)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77410
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
44316303 |
+ |
PFKL |
NNNGGTGCCATTGACCGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
70066368 |
- |
SLC8A3 |
NNNGGTGCCATTGACAGAAA |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000003.12 |
3 |
112834613 |
- |
CD200R1L |
NNNGGTGCCATTGGCCACAA |
NGG |
2 |
0.4356 |
Tier II |
| 4 |
NC_000001.11 |
1 |
204474871 |
- |
PIK3C2B |
NNNGGTGCCATTCACCACAA |
NGG |
2 |
0.393 |
Tier II |
| 5 |
NC_000018.10 |
18 |
11826716 |
+ |
GNAL |
NNNGGTGCCATTTACTGCAA |
NGG |
2 |
0.0923 |
Tier II |
| 6 |
NC_000011.10 |
11 |
32160909 |
+ |
THEM7P |
NNNGGTCCCATTGACCACAA |
NGG |
2 |
0.6417 |
Tier III |
| 7 |
NC_000001.11 |
1 |
208837934 |
- |
LOC107985255 |
NNNTTTGCCATTGACCGCAA |
NGG |
2 |
0.1091 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
77997561 |
- |
Pfkl |
NNNGGTGCCATTGACCGGCA |
NGG |
2 |
0.0276 |
Tier I |
| 2 |
NC_000086.7 |
X |
142684362 |
+ |
Tmem164 |
NNNGGTGCCATTCACAGCAA |
NGG |
2 |
0.4211 |
Tier II |
| 3 |
NC_000074.6 |
8 |
39061972 |
- |
Tusc3 |
NNNGGTGCCACTGACTGCAA |
NGG |
2 |
0.2051 |
Tier II |
| 4 |
NC_000068.7 |
2 |
114160028 |
- |
Aqr |
NNNGGTGCCATTTACCGTAA |
NGG |
2 |
0.1929 |
Tier II |
| 5 |
NC_000080.6 |
14 |
59791862 |
- |
Atp8a2 |
NNNGGTGGCATTGACCTCAA |
NGG |
2 |
0.1607 |
Tier II |
| 6 |
NC_000068.7 |
2 |
172448417 |
+ |
Rtf2 |
NNNGGTGCCAGTGACTGCAA |
NGG |
2 |
0.1231 |
Tier II |
| 7 |
NC_000073.6 |
7 |
88025920 |
+ |
Grm5 |
NNNGGTGCCATTGACCGCTC |
NGG |
2 |
0.1224 |
Tier II |
Other clones with same target sequence:
(none)
