Construct: sgRNA BRDN0001146576
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGATTGGTGAAAGCGTGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS2 (5634)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78007
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
12799228 |
+ |
PRPS2 |
NNNATTGGTGAAAGCGTGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
72366862 |
- |
HDAC8 |
NNNATTTGTTAAAGCGTGAG |
NGG |
2 |
0.4643 |
Tier II |
| 3 |
NC_000007.14 |
7 |
47991800 |
+ |
SUN3 |
NNNGTGGGTGAAAGCGTGAG |
NGG |
2 |
0.3571 |
Tier II |
| 4 |
NC_000011.10 |
11 |
1169784 |
- |
MUC5AC |
NNNGTGGGTGAAAGCGTGAG |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000006.12 |
6 |
68887258 |
- |
ADGRB3 |
NNNATTGTTGAAAGAGTGAG |
NGG |
2 |
0.1389 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
167374166 |
- |
Prps2 |
NNNATCGGTGAAAGTGTGAG |
NGG |
2 |
0.0606 |
Tier I |
| 2 |
NC_000069.6 |
3 |
126439009 |
- |
Arsj |
NNNATTGCTGAAAGCCTGAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000070.6 |
4 |
43561333 |
- |
Tln1 |
NNNATTGGTGATAGCGTTAG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000071.6 |
5 |
12511483 |
+ |
Sema3d |
NNNATTGGTGAAAGCGAGAG |
NAG |
2 |
0.1383 |
Tier II |
| 5 |
NC_000085.6 |
19 |
42091417 |
+ |
Pi4k2a |
NNNATTGGTGACAGCGTGGG |
NGG |
2 |
0.0987 |
Tier II |
| 6 |
NC_000068.7 |
2 |
101449706 |
- |
Gm39886 |
NNNATTGGTGAAAAAGTGAG |
NGG |
2 |
0.1667 |
Tier III |
| 7 |
NC_000075.6 |
9 |
46457645 |
+ |
Gm31432 |
NNNATGGGTGAAAGCCTGAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
