Construct: sgRNA BRDN0001146579

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATCATCTCGGATCGCCACGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKM (1158)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78029

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 45317883 - CKM NNNATCTCGGATCGCCACGG NGG 0 1.0 Tier I
2 NC_000006.12 6 13814628 - LOC107986571 NNNATCTCGGAGCGCCACGG NGG 1 0.5 Tier III
3 NC_000003.12 3 137643184 - LOC105374126 NNNATCTCGGATAGCCACGT NGG 2 0.2692 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 37417308 + Ncald NNNAACTCGGATCCCCACGG NGG 2 0.2143 Tier II
Download CSV

Other clones with same target sequence:

(none)