Construct: sgRNA BRDN0001146583

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCATAGCAACCTGCTCGTCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AATK (9625)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77315

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 81124758 - AATK NNNTAGCAACCTGCTCGTCG NGG 0 1.0 Tier I
2 NC_000003.12 3 32818442 + TRIM71 NNNTAGCAACCTGCTCGACG NGG 1 0.6667 Tier I
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 29086395 + Ntm NNNTAGCAACCTGCTAGTCA NGG 2 0.9375 Tier II
2 NC_000084.6 18 21939785 - Ccdc178 NNNTAGCATCCTGCTCGTCG NCG 2 0.0643 Tier II
3 NC_000075.6 9 29086395 + Gm15520 NNNTAGCAACCTGCTAGTCA NGG 2 0.9375 Tier III
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Other clones with same target sequence:

(none)