Construct: sgRNA BRDN0001146594
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGGAACCATGCTACAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK31 (56164)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77375
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
23769147 |
- |
STK31 |
NNNGGAACCATGCTACAAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
31080733 |
+ |
SMTN |
NNNAGAAGCATGCTACAAGA |
NGG |
2 |
0.5786 |
Tier I |
| 3 |
NC_000002.12 |
2 |
77334767 |
- |
LRRTM4 |
NNNAGTACCATGCTACAAGA |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000008.11 |
8 |
63017981 |
- |
GGH |
NNNGAAAGCATGCTACAAGA |
NGG |
2 |
0.5571 |
Tier II |
| 5 |
NC_000021.9 |
21 |
45844033 |
- |
PCBP3 |
NNNGGAAGAATGCTACAAGA |
NGG |
2 |
0.551 |
Tier II |
| 6 |
NC_000011.10 |
11 |
43772145 |
- |
HSD17B12 |
NNNGGAACCATGCTAAATGA |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000021.9 |
21 |
38632052 |
- |
ERG |
NNNGGAGCCATGTTACAAGA |
NGG |
2 |
0.4941 |
Tier II |
| 8 |
NC_000002.12 |
2 |
229046139 |
+ |
PID1 |
NNNGGAACCATGCTGCAATA |
NGG |
2 |
0.4333 |
Tier II |
| 9 |
NC_000021.9 |
21 |
26142042 |
+ |
APP |
NNNGGAACCATGCAGCAAGA |
NGG |
2 |
0.4024 |
Tier II |
| 10 |
NC_000023.11 |
X |
54606287 |
+ |
GNL3L |
NNNGGAAACATGCTACAACA |
NGG |
2 |
0.2914 |
Tier II |
| 11 |
NC_000005.10 |
5 |
80490727 |
- |
FAM151B |
NNNTGAAACATGCTACAAGA |
NGG |
2 |
0.2364 |
Tier II |
| 12 |
NC_000017.11 |
17 |
19253281 |
- |
EPN2 |
NNNGGATCCATGCTACAACA |
NGG |
2 |
0.1961 |
Tier II |
| 13 |
NC_000022.11 |
22 |
44043104 |
+ |
PARVB |
NNNGGATCCATGCTACAACA |
NGG |
2 |
0.1961 |
Tier II |
| 14 |
NC_000018.10 |
18 |
44699561 |
- |
SETBP1 |
NNNTGACCCATGCTACAAGA |
NGG |
2 |
0.1591 |
Tier II |
| 15 |
NC_000007.14 |
7 |
114115985 |
- |
FOXP2 |
NNNGGAAACATGCTACTAGA |
NGG |
2 |
0.0867 |
Tier II |
| 16 |
NC_000006.12 |
6 |
74607121 |
- |
LOC101928516 |
NNNAGAACCATCCTACAAGA |
NGG |
2 |
0.4765 |
Tier III |
| 17 |
NC_000006.12 |
6 |
74607121 |
- |
LOC105377858 |
NNNAGAACCATCCTACAAGA |
NGG |
2 |
0.4765 |
Tier III |
| 18 |
NC_000006.12 |
6 |
156007548 |
+ |
LOC101928923 |
NNNGGAAGCATGTTACAAGA |
NGG |
2 |
0.45 |
Tier III |
| 19 |
NC_000021.9 |
21 |
26142042 |
+ |
LOC124900466 |
NNNGGAACCATGCAGCAAGA |
NGG |
2 |
0.4024 |
Tier III |
| 20 |
NC_000003.12 |
3 |
15966800 |
- |
LOC107986064 |
NNNGGAACCATGCTACATGT |
NGG |
2 |
0.3 |
Tier III |
| 21 |
NC_000023.11 |
X |
128409222 |
- |
LOC107985698 |
NNNGGAACCATGCTTCAAGA |
NAG |
2 |
0.0519 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
16765937 |
+ |
Vps13a |
NNNGGCACCATGCTACAAGT |
NGG |
2 |
0.2727 |
Tier II |
| 2 |
NC_000085.6 |
19 |
27996252 |
+ |
Rfx3 |
NNNGGAACCATTTTACAAGA |
NGG |
2 |
0.2692 |
Tier II |
| 3 |
NC_000074.6 |
8 |
82339032 |
- |
Il15 |
NNNGAAACCATGCTCCAAGA |
NGG |
2 |
0.2364 |
Tier II |
| 4 |
NC_000067.6 |
1 |
37452187 |
+ |
Mgat4a |
NNNGGAACCATACTACAAGC |
NGG |
2 |
0.2121 |
Tier II |
| 5 |
NC_000073.6 |
7 |
143481320 |
- |
Slc22a18 |
NNNGGAACCATGCTGTAAGA |
NGG |
2 |
0.2 |
Tier II |
| 6 |
NC_000079.6 |
13 |
106830285 |
+ |
Ipo11 |
NNNGGAGCCATGCTACAAGA |
NAG |
2 |
0.183 |
Tier II |
| 7 |
NC_000074.6 |
8 |
58326244 |
+ |
Galntl6 |
NNNGGACCCATGCCACAAGA |
NGG |
2 |
0.125 |
Tier II |
| 8 |
NC_000068.7 |
2 |
120040851 |
- |
Pla2g4b |
NNNAGAACCATGGTACAAGA |
NGG |
2 |
0.1227 |
Tier II |
| 9 |
NC_000076.6 |
10 |
69250125 |
- |
Rhobtb1 |
NNNGGAACCATGCCCCAAGA |
NGG |
2 |
0.0779 |
Tier II |
| 10 |
NC_000073.6 |
7 |
113938164 |
+ |
Spon1 |
NNNGGAACCATGCCAGAAGA |
NGG |
2 |
0.044 |
Tier II |
| 11 |
NC_000077.6 |
11 |
108910756 |
- |
Gm46298 |
NNNGGAACCATGCTACACGA |
NGG |
1 |
0.1905 |
Tier III |
| 12 |
NC_000079.6 |
13 |
106830285 |
+ |
Ipo11-lrrc70 |
NNNGGAGCCATGCTACAAGA |
NAG |
2 |
0.183 |
Tier III |
| 13 |
NC_000068.7 |
2 |
120040851 |
- |
Gm28042 |
NNNAGAACCATGGTACAAGA |
NGG |
2 |
0.1227 |
Tier III |
Other clones with same target sequence:
(none)
