Construct: sgRNA BRDN0001146603

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGACATTGTAAAAGACCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A1 (1457)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76672

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 499838 - CSNK2A1 NNNCATTGTAAAAGACCCTG NGG 0 1.0 Tier I
2 NC_000011.10 11 11352810 - CSNK2A3 NNNCATTGTAAAAGACCCTG NGT 1 0.0161 Tier I
3 NC_000012.12 12 123010054 - PITPNM2 NNNCATTGTCAAAGACCCTG NGC 2 0.0074 Tier I
4 NC_000016.10 16 2930972 - FLYWCH1 NNNCATTTTAAAAGACCCTG NGG 1 0.625 Tier II
5 NC_000003.12 3 53792746 - CACNA1D NNNCACTGGAAAAGACCCTG NGG 2 0.5628 Tier II
6 NC_000009.12 9 109236756 - EPB41L4B NNNCATTGTAAGAAACCCTG NGG 2 0.5417 Tier II
7 NC_000006.12 6 124563544 - NKAIN2 NNNAATTGTGAAAGACCCTG NGG 2 0.4678 Tier II
8 NC_000001.11 1 237826101 + RYR2 NNNCATTGTAAAAGACACTG NGG 1 0.4667 Tier II
9 NC_000008.11 8 36812985 + KCNU1 NNNCATTGTAAAAGACACTA NGG 2 0.4375 Tier II
10 NC_000012.12 12 56002325 - SUOX NNNCATTGTAAAAGACACTA NGG 2 0.4375 Tier II
11 NC_000004.12 4 82434040 + ENOPH1 NNNCATTCTAAAAGACCATG NGG 2 0.3314 Tier II
12 NC_000003.12 3 87913516 + HTR1F NNNCATTGTGAAAGACCATG NGG 2 0.2991 Tier II
13 NC_000005.10 5 146084632 - PLAC8L1 NNNCATTGTACAGGACCCTG NGG 2 0.2609 Tier II
14 NC_000010.11 10 30927689 - ZNF438 NNNCTTTGTAAAAGACCCTT NGG 2 0.2545 Tier II
15 NC_000001.11 1 110021515 + AHCYL1 NNNCATTGTCAGAGACCCTG NGG 2 0.2407 Tier II
16 NC_000002.12 2 105400905 + FHL2 NNNCATTTTAAATGACCCTG NGG 2 0.1875 Tier II
17 NC_000002.12 2 148374398 + MBD5 NNNCATTGTAAAAGACCCTT NAG 2 0.1815 Tier II
18 NC_000003.12 3 71099860 + FOXP1 NNNCAGTGTAAAAGATCCTG NGG 2 0.1758 Tier II
19 NC_000008.11 8 105730193 - ZFPM2 NNNCATTGTAAATGACACTG NGG 2 0.14 Tier II
20 NC_000002.12 2 156492493 + GPD2 NNNCATTGTAAAAGACCATG NAG 2 0.1396 Tier II
21 NC_000008.11 8 10775990 + PINX1 NNNCCTTGTAAAAGCCCCTG NGG 2 0.1364 Tier II
22 NC_000012.12 12 80803544 + LIN7A NNNCTTTGTAATAGACCCTG NGG 2 0.1212 Tier II
23 NC_000015.10 15 67175857 - SMAD3 NNNCATTGTAAAAGAGCCTT NGG 2 0.1077 Tier II
24 NC_000009.12 9 661702 - KANK1 NNNCATTGTAAAAGACAGTG NGG 2 0.0622 Tier II
25 NC_000023.11 X 70738535 + TEX11 NNNCATTGTAAAAGACAGTG NGG 2 0.0622 Tier II
26 NC_000011.10 11 11352810 - GALNT18 NNNCATTGTAAAAGACCCTG NGT 1 0.0161 Tier II
27 NC_000006.12 6 101011735 - LOC107984041 NNNCATTGGAGAAGACCCTG NGG 2 0.4024 Tier III
28 NC_000005.10 5 159823425 + LINC01847 NNNAATTGTAAAAGACCCTC NGG 2 0.3609 Tier III
29 NC_000018.10 18 64419360 - LINC01924 NNNCATGGTGAAAGACCCTG NGG 2 0.3268 Tier III
30 NC_000007.14 7 49541599 - LOC124901804 NNNCATTGTGAAAGACACTG NGG 2 0.2593 Tier III
31 NC_000012.12 12 90089692 - LOC105369890 NNNCATTGTGAAAGACACTG NGG 2 0.2593 Tier III
32 NC_000016.10 16 74199023 - PSMD7-DT NNNCAGTGTACAAGACCCTG NGG 2 0.2286 Tier III
33 NC_000001.11 1 119247559 + WARS2-AS1 NNNCATTGTACAAGACCATG NGG 2 0.2154 Tier III
34 NC_000015.10 15 25513687 + LINC02250 NNNCATTGCAAAAGAGCCTG NGG 2 0.142 Tier III
35 NC_000001.11 1 58813862 - JUN-DT NNNCATTGTACTAGACCCTG NGG 2 0.1333 Tier III
36 NC_000009.12 9 76604314 - LOC105376095 NNNCATTGTCCAAGACCCTG NGG 2 0.1333 Tier III
37 NC_000007.14 7 104959131 + LOC101927902 NNNCATTGTGAAAGACCGTG NGG 2 0.0741 Tier III
38 NC_000003.12 3 106177246 + LOC105374027 NNNCATTTTAAAAGACCCTG NGT 2 0.0101 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 152257475 + Csnk2a1 NNNCATTGTAAAAGACCCTG NGG 0 1.0 Tier I
2 NC_000074.6 8 95477217 - Csnk2a2 NNNCACTGTAAAGGACCCTG NGG 2 0.5929 Tier I
3 NC_000078.6 12 84152562 + Mideas NNNCCTTGTAAAACACCCTG NGG 2 0.2143 Tier I
4 NC_000075.6 9 37642447 + Siae NNNCATTGTAAAAGACCATG NGG 1 0.5385 Tier II
5 NC_000072.6 6 39163956 + Kdm7a NNNCATTGTAAAAGACACTA NGG 2 0.4375 Tier II
6 NC_000086.7 X 92282039 + Mageb18 NNNCATTGTAAAAGAACCTC NGG 2 0.4286 Tier II
7 NC_000083.6 17 75322470 - Ltbp1 NNNCATTCTAAAAGACCTTG NGG 2 0.3956 Tier II
8 NC_000068.7 2 162577928 - Ptprt NNNCATTGTGGAAGACCCTG NGG 2 0.3611 Tier II
9 NC_000069.6 3 28509963 + Tnik NNNCATTGTGGAAGACCCTG NGG 2 0.3611 Tier II
10 NC_000067.6 1 174812091 + Fmn2 NNNCAGTGTAAAAGACTCTG NGG 2 0.2667 Tier II
11 NC_000072.6 6 115813147 - Efcab12 NNNAATTGTAAAAGATCCTG NGG 2 0.2591 Tier II
12 NC_000083.6 17 68343754 - L3mbtl4 NNNTATTGTAAAAGACCCTG NAG 2 0.2074 Tier II
13 NC_000067.6 1 97093256 + Slco6c1 NNNCATTGTACAAGACTCTG NGG 2 0.1867 Tier II
14 NC_000080.6 14 121779719 - Dock9 NNNCAGTGTAAAAGACCCTG NAG 2 0.1481 Tier II
15 NC_000074.6 8 112732422 - Cntnap4 NNNCATTGTAAGAGTCCCTG NGG 2 0.1444 Tier II
16 NC_000070.6 4 82907159 + Frem1 NNNCATTGTAAATGACCCTC NGG 2 0.1286 Tier II
17 NC_000076.6 10 83760694 + 1500009L16Rik NNNCCTTGTAAAAGTCCCTG NGG 2 0.1 Tier II
18 NC_000082.6 16 36186975 - Cstdc4 NNNCATTGTAATAGACCCAG NGG 2 0.0952 Tier II
19 NC_000073.6 7 128708995 + Mcmbp NNNCATTGTAAAAGTCCCTG NAG 2 0.0519 Tier II
20 NC_000073.6 7 49547673 + Nav2 NNNCATTCTAAAAGACCCTG NGC 2 0.0137 Tier II
21 NC_000076.6 10 64961495 - Ctnna3 NNNCATTGTAAAAGACTCTG NGT 2 0.0075 Tier II
22 NC_000086.7 X 35757236 + Gm39502 NNNAATTGTAAAAGACCCTA NGG 2 0.7895 Tier III
23 NC_000083.6 17 16398279 + Gm41534 NNNCATTGTAAAAGACTCTT NGG 2 0.3267 Tier III
24 NC_000076.6 10 67756394 + Gm40681 NNNCATTCTAAAAGTCCCTG NGG 2 0.1231 Tier III
25 NC_000079.6 13 25944973 - Gm11351 NNNCATTGTAAAAGACAGTG NGG 2 0.0622 Tier III
26 NC_000067.6 1 156524541 + Csnk2a3 NNNCATTGTAAAAGACCCTG NGT 1 0.0161 Tier III
27 NC_000080.6 14 14197624 + Csnk2a1-ps1 NNNCATTGTAAAAGACCCTG NGT 1 0.0161 Tier III
28 NC_000086.7 X 16610562 + Csnk2a1-ps NNNCATTGTAAAAGACCCTG NGT 1 0.0161 Tier III
Download CSV

Other clones with same target sequence:

(none)