Construct: sgRNA BRDN0001146603
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACATTGTAAAAGACCCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CSNK2A1 (1457)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000020.11 | 20 | 499838 | - | CSNK2A1 | NNNCATTGTAAAAGACCCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000011.10 | 11 | 11352810 | - | CSNK2A3 | NNNCATTGTAAAAGACCCTG | NGT | 1 | 0.0161 | Tier I |
3 | NC_000012.12 | 12 | 123010054 | - | PITPNM2 | NNNCATTGTCAAAGACCCTG | NGC | 2 | 0.0074 | Tier I |
4 | NC_000016.10 | 16 | 2930972 | - | FLYWCH1 | NNNCATTTTAAAAGACCCTG | NGG | 1 | 0.625 | Tier II |
5 | NC_000003.12 | 3 | 53792746 | - | CACNA1D | NNNCACTGGAAAAGACCCTG | NGG | 2 | 0.5628 | Tier II |
6 | NC_000009.12 | 9 | 109236756 | - | EPB41L4B | NNNCATTGTAAGAAACCCTG | NGG | 2 | 0.5417 | Tier II |
7 | NC_000006.12 | 6 | 124563544 | - | NKAIN2 | NNNAATTGTGAAAGACCCTG | NGG | 2 | 0.4678 | Tier II |
8 | NC_000001.11 | 1 | 237826101 | + | RYR2 | NNNCATTGTAAAAGACACTG | NGG | 1 | 0.4667 | Tier II |
9 | NC_000008.11 | 8 | 36812985 | + | KCNU1 | NNNCATTGTAAAAGACACTA | NGG | 2 | 0.4375 | Tier II |
10 | NC_000012.12 | 12 | 56002325 | - | SUOX | NNNCATTGTAAAAGACACTA | NGG | 2 | 0.4375 | Tier II |
11 | NC_000004.12 | 4 | 82434040 | + | ENOPH1 | NNNCATTCTAAAAGACCATG | NGG | 2 | 0.3314 | Tier II |
12 | NC_000003.12 | 3 | 87913516 | + | HTR1F | NNNCATTGTGAAAGACCATG | NGG | 2 | 0.2991 | Tier II |
13 | NC_000005.10 | 5 | 146084632 | - | PLAC8L1 | NNNCATTGTACAGGACCCTG | NGG | 2 | 0.2609 | Tier II |
14 | NC_000010.11 | 10 | 30927689 | - | ZNF438 | NNNCTTTGTAAAAGACCCTT | NGG | 2 | 0.2545 | Tier II |
15 | NC_000001.11 | 1 | 110021515 | + | AHCYL1 | NNNCATTGTCAGAGACCCTG | NGG | 2 | 0.2407 | Tier II |
16 | NC_000002.12 | 2 | 105400905 | + | FHL2 | NNNCATTTTAAATGACCCTG | NGG | 2 | 0.1875 | Tier II |
17 | NC_000002.12 | 2 | 148374398 | + | MBD5 | NNNCATTGTAAAAGACCCTT | NAG | 2 | 0.1815 | Tier II |
18 | NC_000003.12 | 3 | 71099860 | + | FOXP1 | NNNCAGTGTAAAAGATCCTG | NGG | 2 | 0.1758 | Tier II |
19 | NC_000008.11 | 8 | 105730193 | - | ZFPM2 | NNNCATTGTAAATGACACTG | NGG | 2 | 0.14 | Tier II |
20 | NC_000002.12 | 2 | 156492493 | + | GPD2 | NNNCATTGTAAAAGACCATG | NAG | 2 | 0.1396 | Tier II |
21 | NC_000008.11 | 8 | 10775990 | + | PINX1 | NNNCCTTGTAAAAGCCCCTG | NGG | 2 | 0.1364 | Tier II |
22 | NC_000012.12 | 12 | 80803544 | + | LIN7A | NNNCTTTGTAATAGACCCTG | NGG | 2 | 0.1212 | Tier II |
23 | NC_000015.10 | 15 | 67175857 | - | SMAD3 | NNNCATTGTAAAAGAGCCTT | NGG | 2 | 0.1077 | Tier II |
24 | NC_000009.12 | 9 | 661702 | - | KANK1 | NNNCATTGTAAAAGACAGTG | NGG | 2 | 0.0622 | Tier II |
25 | NC_000023.11 | X | 70738535 | + | TEX11 | NNNCATTGTAAAAGACAGTG | NGG | 2 | 0.0622 | Tier II |
26 | NC_000011.10 | 11 | 11352810 | - | GALNT18 | NNNCATTGTAAAAGACCCTG | NGT | 1 | 0.0161 | Tier II |
27 | NC_000006.12 | 6 | 101011735 | - | LOC107984041 | NNNCATTGGAGAAGACCCTG | NGG | 2 | 0.4024 | Tier III |
28 | NC_000005.10 | 5 | 159823425 | + | LINC01847 | NNNAATTGTAAAAGACCCTC | NGG | 2 | 0.3609 | Tier III |
29 | NC_000018.10 | 18 | 64419360 | - | LINC01924 | NNNCATGGTGAAAGACCCTG | NGG | 2 | 0.3268 | Tier III |
30 | NC_000007.14 | 7 | 49541599 | - | LOC124901804 | NNNCATTGTGAAAGACACTG | NGG | 2 | 0.2593 | Tier III |
31 | NC_000012.12 | 12 | 90089692 | - | LOC105369890 | NNNCATTGTGAAAGACACTG | NGG | 2 | 0.2593 | Tier III |
32 | NC_000016.10 | 16 | 74199023 | - | PSMD7-DT | NNNCAGTGTACAAGACCCTG | NGG | 2 | 0.2286 | Tier III |
33 | NC_000001.11 | 1 | 119247559 | + | WARS2-AS1 | NNNCATTGTACAAGACCATG | NGG | 2 | 0.2154 | Tier III |
34 | NC_000015.10 | 15 | 25513687 | + | LINC02250 | NNNCATTGCAAAAGAGCCTG | NGG | 2 | 0.142 | Tier III |
35 | NC_000001.11 | 1 | 58813862 | - | JUN-DT | NNNCATTGTACTAGACCCTG | NGG | 2 | 0.1333 | Tier III |
36 | NC_000009.12 | 9 | 76604314 | - | LOC105376095 | NNNCATTGTCCAAGACCCTG | NGG | 2 | 0.1333 | Tier III |
37 | NC_000007.14 | 7 | 104959131 | + | LOC101927902 | NNNCATTGTGAAAGACCGTG | NGG | 2 | 0.0741 | Tier III |
38 | NC_000003.12 | 3 | 106177246 | + | LOC105374027 | NNNCATTTTAAAAGACCCTG | NGT | 2 | 0.0101 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000068.7 | 2 | 152257475 | + | Csnk2a1 | NNNCATTGTAAAAGACCCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000074.6 | 8 | 95477217 | - | Csnk2a2 | NNNCACTGTAAAGGACCCTG | NGG | 2 | 0.5929 | Tier I |
3 | NC_000078.6 | 12 | 84152562 | + | Mideas | NNNCCTTGTAAAACACCCTG | NGG | 2 | 0.2143 | Tier I |
4 | NC_000075.6 | 9 | 37642447 | + | Siae | NNNCATTGTAAAAGACCATG | NGG | 1 | 0.5385 | Tier II |
5 | NC_000072.6 | 6 | 39163956 | + | Kdm7a | NNNCATTGTAAAAGACACTA | NGG | 2 | 0.4375 | Tier II |
6 | NC_000086.7 | X | 92282039 | + | Mageb18 | NNNCATTGTAAAAGAACCTC | NGG | 2 | 0.4286 | Tier II |
7 | NC_000083.6 | 17 | 75322470 | - | Ltbp1 | NNNCATTCTAAAAGACCTTG | NGG | 2 | 0.3956 | Tier II |
8 | NC_000068.7 | 2 | 162577928 | - | Ptprt | NNNCATTGTGGAAGACCCTG | NGG | 2 | 0.3611 | Tier II |
9 | NC_000069.6 | 3 | 28509963 | + | Tnik | NNNCATTGTGGAAGACCCTG | NGG | 2 | 0.3611 | Tier II |
10 | NC_000067.6 | 1 | 174812091 | + | Fmn2 | NNNCAGTGTAAAAGACTCTG | NGG | 2 | 0.2667 | Tier II |
11 | NC_000072.6 | 6 | 115813147 | - | Efcab12 | NNNAATTGTAAAAGATCCTG | NGG | 2 | 0.2591 | Tier II |
12 | NC_000083.6 | 17 | 68343754 | - | L3mbtl4 | NNNTATTGTAAAAGACCCTG | NAG | 2 | 0.2074 | Tier II |
13 | NC_000067.6 | 1 | 97093256 | + | Slco6c1 | NNNCATTGTACAAGACTCTG | NGG | 2 | 0.1867 | Tier II |
14 | NC_000080.6 | 14 | 121779719 | - | Dock9 | NNNCAGTGTAAAAGACCCTG | NAG | 2 | 0.1481 | Tier II |
15 | NC_000074.6 | 8 | 112732422 | - | Cntnap4 | NNNCATTGTAAGAGTCCCTG | NGG | 2 | 0.1444 | Tier II |
16 | NC_000070.6 | 4 | 82907159 | + | Frem1 | NNNCATTGTAAATGACCCTC | NGG | 2 | 0.1286 | Tier II |
17 | NC_000076.6 | 10 | 83760694 | + | 1500009L16Rik | NNNCCTTGTAAAAGTCCCTG | NGG | 2 | 0.1 | Tier II |
18 | NC_000082.6 | 16 | 36186975 | - | Cstdc4 | NNNCATTGTAATAGACCCAG | NGG | 2 | 0.0952 | Tier II |
19 | NC_000073.6 | 7 | 128708995 | + | Mcmbp | NNNCATTGTAAAAGTCCCTG | NAG | 2 | 0.0519 | Tier II |
20 | NC_000073.6 | 7 | 49547673 | + | Nav2 | NNNCATTCTAAAAGACCCTG | NGC | 2 | 0.0137 | Tier II |
21 | NC_000076.6 | 10 | 64961495 | - | Ctnna3 | NNNCATTGTAAAAGACTCTG | NGT | 2 | 0.0075 | Tier II |
22 | NC_000086.7 | X | 35757236 | + | Gm39502 | NNNAATTGTAAAAGACCCTA | NGG | 2 | 0.7895 | Tier III |
23 | NC_000083.6 | 17 | 16398279 | + | Gm41534 | NNNCATTGTAAAAGACTCTT | NGG | 2 | 0.3267 | Tier III |
24 | NC_000076.6 | 10 | 67756394 | + | Gm40681 | NNNCATTCTAAAAGTCCCTG | NGG | 2 | 0.1231 | Tier III |
25 | NC_000079.6 | 13 | 25944973 | - | Gm11351 | NNNCATTGTAAAAGACAGTG | NGG | 2 | 0.0622 | Tier III |
26 | NC_000067.6 | 1 | 156524541 | + | Csnk2a3 | NNNCATTGTAAAAGACCCTG | NGT | 1 | 0.0161 | Tier III |
27 | NC_000080.6 | 14 | 14197624 | + | Csnk2a1-ps1 | NNNCATTGTAAAAGACCCTG | NGT | 1 | 0.0161 | Tier III |
28 | NC_000086.7 | X | 16610562 | + | Csnk2a1-ps | NNNCATTGTAAAAGACCCTG | NGT | 1 | 0.0161 | Tier III |