Construct: sgRNA BRDN0001146604
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCACAGCCGGACTATCCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK5 (8550)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76447
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
111883672 |
+ |
MAPKAPK5 |
NNNCAGCCGGACTATCCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
22882441 |
+ |
EPHB2 |
NNNCAGCCGGACCAGCCCAA |
NGG |
2 |
0.0395 |
Tier I |
| 3 |
NC_000007.14 |
7 |
39398717 |
+ |
POU6F2 |
NNNCAGTCGGACTATCCCAG |
NGG |
2 |
0.6213 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
121531630 |
- |
Mapkapk5 |
NNNTAGTCGGACTATCCCAA |
NGG |
2 |
0.65 |
Tier I |
| 2 |
NC_000079.6 |
13 |
68751503 |
+ |
Adcy2 |
NNNCAGCTAGACTATCCCAA |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000084.6 |
18 |
45629014 |
- |
Kcnn2 |
NNNCAGCAAGACTATCCCAA |
NGG |
2 |
0.4179 |
Tier II |
| 4 |
NC_000079.6 |
13 |
73609274 |
- |
Clptm1l |
NNNCAGCAGGACTATCCCTA |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000067.6 |
1 |
158259770 |
+ |
Brinp2 |
NNNCAGCCTGATTATCCCAA |
NGG |
2 |
0.2872 |
Tier II |
| 6 |
NC_000080.6 |
14 |
30523864 |
- |
Dcp1a |
NNNCAGCCGGCCTATCCCAA |
NAG |
2 |
0.1037 |
Tier II |
Other clones with same target sequence:
(none)
