Construct: sgRNA BRDN0001146610
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCGGGAACCTTCAACCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RIPK4 (54101)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75971
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
41743887 |
- |
RIPK4 |
NNNCGGGAACCTTCAACCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
166166656 |
+ |
TBXT |
NNNCGGGAGCCTTCATCCAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000009.12 |
9 |
93131535 |
+ |
NINJ1 |
NNNCAGGAACCTTCAACCTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000010.11 |
10 |
71366601 |
+ |
SLC29A3 |
NNNGGGGAAACTTCAACCAG |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000008.11 |
8 |
118506874 |
+ |
SAMD12 |
NNNCAGGAACCTTCAACCAC |
NGG |
2 |
0.3714 |
Tier II |
| 6 |
NC_000002.12 |
2 |
10323245 |
- |
HPCAL1 |
NNNCGGGAACCTGCAACCAA |
NGG |
2 |
0.2446 |
Tier II |
| 7 |
NC_000010.11 |
10 |
14762353 |
+ |
FAM107B |
NNNCGGGAACCTGCACCCAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000016.10 |
16 |
87711900 |
+ |
KLHDC4 |
NNNGGGGAACCTTCACCCAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000012.12 |
12 |
130762182 |
- |
LOC105370080 |
NNNAGGGAACCTGCAACCAG |
NGG |
2 |
0.2197 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
57023853 |
- |
Khsrp |
NNNCTGGACCCTTCAACCAG |
NGG |
2 |
0.1714 |
Tier I |
| 2 |
NC_000068.7 |
2 |
91937883 |
+ |
Dgkz |
NNNACGGAACCTTCAACCAG |
NGG |
2 |
0.6617 |
Tier II |
| 3 |
NC_000082.6 |
16 |
94247262 |
+ |
Hlcs |
NNNTGGGAGCCTTCAACCAG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000077.6 |
11 |
68348381 |
- |
Ntn1 |
NNNGGGGAACCTTCAACCAA |
NGG |
2 |
0.4688 |
Tier II |
| 5 |
NC_000080.6 |
14 |
69823638 |
- |
Rhobtb2 |
NNNCGGGAACCTTTAAGCAG |
NGG |
2 |
0.0431 |
Tier II |
| 6 |
NC_000071.6 |
5 |
76159653 |
+ |
Gm33938 |
NNNCCAGAACCTTCAACCAG |
NGG |
2 |
0.7857 |
Tier III |
| 7 |
NC_000072.6 |
6 |
4359074 |
+ |
Gm8602 |
NNNCTGGACCCTTCAACCAG |
NGG |
2 |
0.1714 |
Tier III |
Other clones with same target sequence:
(none)
