Construct: sgRNA BRDN0001146614
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATTGAGCGATTTCGCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R3 (8503)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76344
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46061957 |
- |
PIK3R3 |
NNNTGAGCGATTTCGCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
46061957 |
- |
P3R3URF-PIK3R3 |
NNNTGAGCGATTTCGCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000006.12 |
6 |
12725191 |
- |
PHACTR1 |
NNNTGACCGATTTCACAGAG |
NGG |
2 |
0.6471 |
Tier II |
| 4 |
NC_000013.11 |
13 |
110914328 |
- |
ANKRD10 |
NNNCGAGCGATTTCGCAGAG |
NCG |
2 |
0.0693 |
Tier II |
| 5 |
NC_000006.12 |
6 |
12725191 |
- |
LOC107984015 |
NNNTGACCGATTTCACAGAG |
NGG |
2 |
0.6471 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
116286171 |
+ |
Pik3r3 |
NNNCGAGCGCTTTCGCAGAG |
NGG |
2 |
0.2157 |
Tier I |
| 2 |
NC_000080.6 |
14 |
119838856 |
- |
Hs6st3 |
NNNTGAGCTATTTCACAGAG |
NGG |
2 |
0.502 |
Tier II |
| 3 |
NC_000074.6 |
8 |
64831055 |
+ |
Klhl2 |
NNNTGAGCCAGTTCGCAGAG |
NGG |
2 |
0.2154 |
Tier II |
| 4 |
NC_000080.6 |
14 |
16580564 |
- |
Rarb |
NNNTGAGCAATTTCTCAGAG |
NGG |
2 |
0.0918 |
Tier II |
| 5 |
NC_000075.6 |
9 |
67333020 |
- |
Tln2 |
NNNTGAGCTATTTCTCAGAG |
NGG |
2 |
0.0762 |
Tier II |
| 6 |
NC_000085.6 |
19 |
9942135 |
- |
Gm36913 |
NNNTGAGGGATTTCCCAGAG |
NGG |
2 |
0.1753 |
Tier III |
| 7 |
NC_000073.6 |
7 |
72310887 |
+ |
Gm39035 |
NNNTGAGCGAGTTCGCAGGG |
NGG |
2 |
0.15 |
Tier III |
Other clones with same target sequence:
(none)
