Construct: sgRNA BRDN0001146615
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGTGCTCAAAATCAGATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK2 (5062)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77569
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
196801971 |
- |
PAK2 |
NNNTGCTCAAAATCAGATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
111254384 |
+ |
CAPN6 |
NNNTGCCCACAATCAGATGG |
NGG |
2 |
0.2292 |
Tier I |
| 3 |
NC_000001.11 |
1 |
196905127 |
+ |
CFHR4 |
NNNTGCTCAAAATCAGATGT |
NGA |
2 |
0.0486 |
Tier I |
| 4 |
NC_000005.10 |
5 |
5467279 |
+ |
ICE1 |
NNNTGATTAAAATCAGATGG |
NGG |
2 |
0.8125 |
Tier II |
| 5 |
NC_000010.11 |
10 |
86926448 |
+ |
BMPR1A |
NNNTGCTTAAAATCAGAAGG |
NGG |
2 |
0.5833 |
Tier II |
| 6 |
NC_000001.11 |
1 |
67018402 |
+ |
SLC35D1 |
NNNTGCTCAAAATCAGAAGT |
NGG |
2 |
0.4667 |
Tier II |
| 7 |
NC_000010.11 |
10 |
70130752 |
- |
AIFM2 |
NNNAGTTCAAAATCAGATGG |
NGG |
2 |
0.4422 |
Tier II |
| 8 |
NC_000001.11 |
1 |
206936067 |
- |
PIGR |
NNNTGCTCAGAATAAGATGG |
NGG |
2 |
0.1944 |
Tier II |
| 9 |
NC_000022.11 |
22 |
32635748 |
+ |
SYN3 |
NNNTTCTCCAAATCAGATGG |
NGG |
2 |
0.1714 |
Tier II |
| 10 |
NC_000003.12 |
3 |
130926274 |
- |
ATP2C1 |
NNNTGCTCAAATTAAGATGG |
NGG |
2 |
0.1167 |
Tier II |
| 11 |
NC_000013.11 |
13 |
49738166 |
+ |
KPNA3 |
NNNTGCTAAAAATCAGCTGG |
NGG |
2 |
0.1147 |
Tier II |
| 12 |
NC_000011.10 |
11 |
133032047 |
+ |
OPCML |
NNNTGTTCAAAATCAGATGG |
NGA |
2 |
0.0645 |
Tier II |
| 13 |
NC_000006.12 |
6 |
151347193 |
- |
AKAP12 |
NNNTGCTCAAAAGCTGATGG |
NGG |
2 |
0.0522 |
Tier II |
| 14 |
NC_000006.12 |
6 |
57296901 |
+ |
PRIM2 |
NNNTGCTCAAAAACAGATGG |
NGA |
2 |
0.0481 |
Tier II |
| 15 |
NC_000005.10 |
5 |
79455274 |
+ |
HOMER1 |
NNNTGCTCAAGATCAGATGG |
NGA |
2 |
0.0451 |
Tier II |
| 16 |
NC_000017.11 |
17 |
55808358 |
- |
PCTP |
NNNTGCTTAAAATCAGATGG |
NTG |
2 |
0.0341 |
Tier II |
| 17 |
NC_000004.12 |
4 |
106341592 |
+ |
AIMP1 |
NNNTGCTGAAAATCAGATGG |
NTG |
2 |
0.025 |
Tier II |
| 18 |
NC_000012.12 |
12 |
85149454 |
+ |
LRRIQ1 |
NNNTGCTGAAAATCAGATGG |
NTG |
2 |
0.025 |
Tier II |
| 19 |
NC_000001.11 |
1 |
246372343 |
- |
SMYD3 |
NNNTGCTCAAACTCAGATGG |
NTG |
2 |
0.0103 |
Tier II |
| 20 |
NC_000020.11 |
20 |
13945246 |
+ |
SEL1L2 |
NNNTGCTCAAAATCAGTTGG |
NGT |
2 |
0.0022 |
Tier II |
| 21 |
NC_000003.12 |
3 |
127952993 |
- |
KBTBD12 |
NNNTGCTCAAAATCACATGG |
NGC |
2 |
0.0 |
Tier II |
| 22 |
NC_000016.10 |
16 |
7642355 |
+ |
RBFOX1 |
NNNTGCTCAAAATCAGATGG |
NCT |
2 |
0.0 |
Tier II |
| 23 |
NC_000018.10 |
18 |
72785928 |
- |
NETO1 |
NNNTGTTCAAAATCACATGG |
NGG |
2 |
0.0 |
Tier II |
| 24 |
NC_000015.10 |
15 |
21294986 |
+ |
LOC646214 |
NNNTGTTCAAAATCAGATGG |
NGG |
1 |
0.9286 |
Tier III |
| 25 |
NT_187382.1 |
15 |
22127 |
+ |
LOC102723432 |
NNNTGTTCAAAATCAGATGG |
NGG |
1 |
0.9286 |
Tier III |
| 26 |
NC_000004.12 |
4 |
83998308 |
+ |
LINC02994 |
NNNTGGTGAAAATCAGATGG |
NGG |
2 |
0.3214 |
Tier III |
| 27 |
NC_000007.14 |
7 |
143464540 |
+ |
EPHA1-AS1 |
NNNTTCTCAAAACCAGATGG |
NGG |
2 |
0.2368 |
Tier III |
| 28 |
NC_000002.12 |
2 |
18679827 |
+ |
LOC105373456 |
NNNTGCTCAAATTCAGATGG |
NAG |
2 |
0.0864 |
Tier III |
| 29 |
NC_000015.10 |
15 |
39007591 |
- |
LOC105370777 |
NNNTGCTCAAAATCTGATGG |
NGC |
2 |
0.0044 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
32044538 |
+ |
Pak2 |
NNNTGCTCAAAATCAGATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000081.6 |
15 |
101203079 |
+ |
Acvr1b |
NNNTGCTCCAAATCAGAGGG |
NGG |
2 |
0.1905 |
Tier I |
| 3 |
NC_000084.6 |
18 |
35307698 |
+ |
Sil1 |
NNNTGCTTAAAATTAGATGG |
NGG |
2 |
0.6417 |
Tier II |
| 4 |
NC_000071.6 |
5 |
43760051 |
- |
Fbxl5 |
NNNGGCTCAAAATTAGATGG |
NGG |
2 |
0.4583 |
Tier II |
| 5 |
NC_000067.6 |
1 |
124430149 |
+ |
Dpp10 |
NNNTGCTCAGAATCAGAAGG |
NGG |
2 |
0.3704 |
Tier II |
| 6 |
NC_000079.6 |
13 |
99384312 |
- |
Mrps27 |
NNNTGCTGAGAATCAGATGG |
NGG |
2 |
0.3571 |
Tier II |
| 7 |
NC_000073.6 |
7 |
56191455 |
- |
Herc2 |
NNNAGCTCAAAATCAGATAG |
NGG |
2 |
0.3401 |
Tier II |
| 8 |
NC_000084.6 |
18 |
66057437 |
+ |
Ccbe1 |
NNNTGCTCAAAATAAGATAG |
NGG |
2 |
0.25 |
Tier II |
| 9 |
NC_000074.6 |
8 |
93328237 |
- |
Ces1g |
NNNTGCTCAGCATCAGATGG |
NGG |
2 |
0.2222 |
Tier II |
| 10 |
NC_000081.6 |
15 |
7260312 |
- |
Egflam |
NNNGGCTCAAAAGCAGATGG |
NGG |
2 |
0.163 |
Tier II |
| 11 |
NC_000077.6 |
11 |
107334200 |
+ |
Pitpnc1 |
NNNTTCTCAAAAGCAGATGG |
NGG |
2 |
0.0783 |
Tier II |
| 12 |
NC_000079.6 |
13 |
112562663 |
+ |
Il31ra |
NNNTGCTCAAAATCAGAAGG |
NTG |
2 |
0.026 |
Tier II |
| 13 |
NC_000080.6 |
14 |
119809284 |
+ |
Hs6st3 |
NNNTGCTCCAAATCAGATGG |
NGC |
2 |
0.0127 |
Tier II |
| 14 |
NC_000067.6 |
1 |
15708918 |
- |
Kcnb2 |
NNNTGCTCAACATCACATGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000068.7 |
2 |
103940730 |
+ |
Gm39896 |
NNNTGCTCAAAGTCAAATGG |
NGG |
2 |
0.7222 |
Tier III |
| 16 |
NC_000082.6 |
16 |
30929296 |
- |
Gm46565 |
NNNTACTCAAAACCAGATGG |
NGG |
2 |
0.6842 |
Tier III |
| 17 |
NC_000067.6 |
1 |
124430149 |
+ |
Gm41941 |
NNNTGCTCAGAATCAGAAGG |
NGG |
2 |
0.3704 |
Tier III |
| 18 |
NC_000074.6 |
8 |
80969669 |
+ |
Gm31223 |
NNNTGCTGAAAATCTGATGG |
NGG |
2 |
0.1286 |
Tier III |
| 19 |
NC_000080.6 |
14 |
119809284 |
+ |
4930404K13Rik |
NNNTGCTCCAAATCAGATGG |
NGC |
2 |
0.0127 |
Tier III |
| 20 |
NC_000068.7 |
2 |
94235969 |
- |
Mir670hg |
NNNTGCTCAAAAAGAGATGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
