Construct: sgRNA BRDN0001146616
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATTCCTTAAGCACTAGTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K2 (10746)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75510
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
127322159 |
- |
MAP3K2 |
NNNTCCTTAAGCACTAGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
51779083 |
+ |
PKHD1 |
NNNTCCTCAAGAACTAGTAG |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000011.10 |
11 |
96211794 |
+ |
MAML2 |
NNNTCTTTAAGCACTAGTTG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000014.9 |
14 |
93470483 |
+ |
UNC79 |
NNNTCCTTAAACTCTAGTAG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000004.12 |
4 |
6167459 |
- |
JAKMIP1 |
NNNTCCTTAACCACTATTAG |
NGG |
2 |
0.1071 |
Tier II |
| 6 |
NC_000011.10 |
11 |
66100455 |
+ |
PACS1 |
NNNTCCTTGAGCACTAGTAG |
NTG |
2 |
0.026 |
Tier II |
| 7 |
NC_000003.12 |
3 |
41490830 |
- |
ULK4 |
NNNTCCTTAAGCACCAGTAG |
NGT |
2 |
0.0044 |
Tier II |
| 8 |
NC_000002.12 |
2 |
10576901 |
- |
NOL10 |
NNNTCCTTAAGCAGTAGTAG |
NGA |
2 |
0.0 |
Tier II |
| 9 |
NC_000012.12 |
12 |
81090740 |
+ |
ACSS3 |
NNNTCCTTAAGCTGTAGTAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000023.11 |
X |
8371182 |
- |
LOC107985675 |
NNNTCCTTAAGCACTGGTAG |
NGG |
1 |
0.1923 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
32212056 |
+ |
Map3k2 |
NNNTCCTTAAGCACTAGTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000072.6 |
6 |
108315347 |
- |
Itpr1 |
NNNTCCTTAAACACTGGTAG |
NGG |
2 |
0.1923 |
Tier II |
| 3 |
NC_000071.6 |
5 |
49115296 |
- |
Kcnip4 |
NNNTCCTTATGCACTAGTAG |
NGA |
2 |
0.0613 |
Tier II |
Other clones with same target sequence:
(none)
