Construct: sgRNA BRDN0001146621
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACAATACGATGATCAAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MYLK4 (340156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75816
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
2688930 |
+ |
MYLK4 |
NNNAATACGATGATCAAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
20139688 |
- |
OR4N5 |
NNNAATATGATGATCAAATT |
NGG |
2 |
0.6125 |
Tier II |
| 3 |
NC_000023.11 |
X |
57342120 |
- |
FAAH2 |
NNNAAAACCATGATCAAATG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000010.11 |
10 |
15362136 |
- |
FAM171A1 |
NNNGATAAGATGATCAAATG |
NGG |
2 |
0.4063 |
Tier II |
| 5 |
NC_000003.12 |
3 |
97845543 |
- |
CRYBG3 |
NNNAAGACAATGATCAAATG |
NGG |
2 |
0.3673 |
Tier II |
| 6 |
NC_000002.12 |
2 |
189828377 |
- |
PMS1 |
NNNAATACAATGATCAAATC |
NGG |
2 |
0.2755 |
Tier II |
| 7 |
NC_000003.12 |
3 |
66207403 |
- |
SLC25A26 |
NNNAATACAATGATCAAATG |
NAG |
2 |
0.1667 |
Tier II |
| 8 |
NC_000012.12 |
12 |
20836348 |
- |
SLCO1B3 |
NNNAATACCATGATCAAATG |
NGA |
2 |
0.0374 |
Tier II |
| 9 |
NC_000012.12 |
12 |
20836348 |
- |
SLCO1B3-SLCO1B7 |
NNNAATACCATGATCAAATG |
NGA |
2 |
0.0374 |
Tier II |
| 10 |
NC_000003.12 |
3 |
97845543 |
- |
LOC105373994 |
NNNAAGACAATGATCAAATG |
NGG |
2 |
0.3673 |
Tier III |
| 11 |
NC_000006.12 |
6 |
140618668 |
- |
LOC105378026 |
NNNTATACCATGATCAAATG |
NGG |
2 |
0.3427 |
Tier III |
| 12 |
NC_000006.12 |
6 |
140618668 |
- |
LOC105378027 |
NNNTATACCATGATCAAATG |
NGG |
2 |
0.3427 |
Tier III |
| 13 |
NC_000013.11 |
13 |
78358718 |
+ |
OBI1-AS1 |
NNNATTATGATGATCAAATG |
NGG |
2 |
0.3182 |
Tier III |
| 14 |
NC_000008.11 |
8 |
138002077 |
+ |
LOC401478 |
NNNAATACCATGACCAAATG |
NGG |
2 |
0.1538 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
106409725 |
+ |
Usp24 |
NNNAATACGATGAGCAAATA |
NGG |
2 |
0.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
42036401 |
+ |
Vmn2r59 |
NNNAATAGGAAGATCAAATG |
NGG |
2 |
0.4821 |
Tier II |
| 3 |
NC_000070.6 |
4 |
94530246 |
- |
Caap1 |
NNNCATATGATGATCAAATG |
NGG |
2 |
0.3088 |
Tier II |
| 4 |
NC_000069.6 |
3 |
75325457 |
- |
Wdr49 |
NNNAATAGGAGGATCAAATG |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000068.7 |
2 |
60028670 |
- |
Baz2b |
NNNAATAGGATGATGAAATG |
NGG |
2 |
0.0321 |
Tier II |
| 6 |
NC_000068.7 |
2 |
63190150 |
- |
Gm34110 |
NNNAATACGATGATCAGATG |
NGG |
1 |
0.1765 |
Tier III |
| 7 |
NC_000076.6 |
10 |
31035546 |
- |
Gm30676 |
NNNAATACGGTGATGAAATG |
NGG |
2 |
0.0278 |
Tier III |
Other clones with same target sequence:
(none)
