Construct: sgRNA BRDN0001146622
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGTCGAATATCATGCTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADK (132)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75744
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
74314709 |
+ |
ADK |
NNNGTCGAATATCATGCTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
592240 |
- |
HCN2 |
NNNGTCGAAGGTCATGCTGG |
NGG |
2 |
0.325 |
Tier II |
| 3 |
NC_000014.9 |
14 |
80226392 |
+ |
DIO2 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000023.11 |
X |
75447374 |
- |
ZDHHC15 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
74043333 |
+ |
LRRIQ3 |
NNNGTCAAATATGATGCTGG |
NGG |
2 |
0.1364 |
Tier II |
| 6 |
NC_000005.10 |
5 |
170639192 |
- |
KCNIP1 |
NNNGTCGAATGTCATTCTGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000004.12 |
4 |
177738231 |
- |
LINC01098 |
NNNGTAGAAAATCATGCTGG |
NGG |
2 |
0.7959 |
Tier III |
| 8 |
NC_000001.11 |
1 |
37369972 |
- |
LOC107984942 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier III |
| 9 |
NC_000005.10 |
5 |
106914587 |
+ |
LINC01950 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier III |
| 10 |
NC_000014.9 |
14 |
80226392 |
+ |
DIO2-AS1 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier III |
| 11 |
NC_000021.9 |
21 |
15581422 |
- |
LOC105369302 |
NNNGTGGAATATCATGATGG |
NGG |
2 |
0.2333 |
Tier III |
| 12 |
NC_000008.11 |
8 |
76441924 |
+ |
LINC01111 |
NNNCTCGAATATCATGCTGG |
NAG |
2 |
0.1373 |
Tier III |
| 13 |
NC_000003.12 |
3 |
35340379 |
+ |
LOC101928135 |
NNNGTAGAATATCATGCTGG |
NGT |
2 |
0.015 |
Tier III |
| 14 |
NC_000005.10 |
5 |
170639192 |
- |
KCNIP1-AS1 |
NNNGTCGAATGTCATTCTGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
21169842 |
+ |
Adk |
NNNGTTGAATATCATGCTGG |
NGG |
1 |
0.9286 |
Tier I |
| 2 |
NC_000073.6 |
7 |
29379537 |
+ |
Sipa1l3 |
NNNGCCGAATAGCATGCTGG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000077.6 |
11 |
36124973 |
+ |
Tenm2 |
NNNGTAGAATATCATGCTGG |
NAG |
2 |
0.2407 |
Tier II |
| 4 |
NC_000073.6 |
7 |
63473979 |
+ |
Otud7a |
NNNGTCGAATCTCAAGCTGG |
NGG |
2 |
0.2316 |
Tier II |
| 5 |
NC_000070.6 |
4 |
98231951 |
+ |
Gm12691 |
NNNGAGGAATATCATGCTGG |
NGG |
2 |
0.25 |
Tier III |
Other clones with same target sequence:
(none)
