Construct: sgRNA BRDN0001146623
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGACGGTCACTAATGTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TBRG4 (9238)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77705
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
45105509 |
+ |
TBRG4 |
NNNGACGGTCACTAATGTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
36681913 |
+ |
SLC25A21 |
NNNGAGGGTCATTAATGTGT |
NGG |
2 |
0.2692 |
Tier II |
| 3 |
NC_000007.14 |
7 |
39419807 |
- |
POU6F2 |
NNNCCCGGTCACTAATGTGT |
NGG |
2 |
0.2647 |
Tier II |
| 4 |
NC_000001.11 |
1 |
76078290 |
- |
ST6GALNAC3 |
NNNGAGGGTCACTATTGTGT |
NGG |
2 |
0.1 |
Tier II |
| 5 |
NC_000007.14 |
7 |
39419807 |
- |
LOC105375238 |
NNNCCCGGTCACTAATGTGT |
NGG |
2 |
0.2647 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
3158586 |
+ |
AU018091 |
NNNGAAGGTCACTAAGGTGT |
NGG |
2 |
0.3214 |
Tier I |
| 2 |
NC_000079.6 |
13 |
9924779 |
+ |
Chrm3 |
NNNGGCAGTCACTAATGTGT |
NGG |
2 |
0.72 |
Tier II |
| 3 |
NC_000070.6 |
4 |
147210575 |
- |
Zfp978 |
NNNGACGGTCACCAAGGTGT |
NGG |
2 |
0.2733 |
Tier II |
| 4 |
NC_000076.6 |
10 |
62372520 |
- |
Hk1 |
NNNGACGGTCACTATTGTGT |
NGG |
1 |
0.2 |
Tier II |
| 5 |
NC_000083.6 |
17 |
80261483 |
+ |
Dhx57 |
NNNGACGGTCACTAATCTGC |
NGG |
2 |
0.0214 |
Tier II |
Other clones with same target sequence:
(none)
