Construct: sgRNA BRDN0001146625
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGAGAAGGACCGCCACAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAB1 (10454)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77790
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
39417808 |
- |
TAB1 |
NNNGAGAAGGACCGCCACAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
24877731 |
+ |
BCAT1 |
NNNGAGAAGTACTGCCACAA |
NGG |
2 |
0.5688 |
Tier II |
| 3 |
NC_000003.12 |
3 |
126187421 |
- |
ALDH1L1 |
NNNGAGAAGGAAAGCCACAA |
NGG |
2 |
0.2747 |
Tier II |
| 4 |
NC_000019.10 |
19 |
29813065 |
- |
CCNE1 |
NNNGAGAAGGACCTCCACAG |
NGG |
2 |
0.2039 |
Tier II |
| 5 |
NC_000021.9 |
21 |
40684437 |
+ |
DSCAM |
NNNCAGAAGGACAGCCACAA |
NGG |
2 |
0.2036 |
Tier II |
| 6 |
NC_000001.11 |
1 |
108890707 |
- |
GPSM2 |
NNNGAGGAGGACCTCCACAA |
NGG |
2 |
0.1882 |
Tier II |
| 7 |
NC_000002.12 |
2 |
231000275 |
- |
SPATA3 |
NNNGAGAAGGCCCCCCACAA |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000008.11 |
8 |
140540651 |
- |
AGO2 |
NNNGAGAGGGACGGCCACAA |
NGG |
2 |
0.1 |
Tier II |
| 9 |
NC_000001.11 |
1 |
241275542 |
- |
RGS7 |
NNNGAGAAGGACGGCCTCAA |
NGG |
2 |
0.0182 |
Tier II |
| 10 |
NC_000016.10 |
16 |
88762841 |
+ |
PIEZO1 |
NNNGAGAAGGACAGCCACAA |
NGT |
2 |
0.0062 |
Tier II |
| 11 |
NC_000009.12 |
9 |
42718727 |
- |
LOC124902164 |
NNNGGGAAGGACTGCCACAA |
NGG |
2 |
0.504 |
Tier III |
| 12 |
NC_000012.12 |
12 |
28755266 |
+ |
LOC105369711 |
NNNGAGAAGGGCTGCCACAA |
NGG |
2 |
0.455 |
Tier III |
| 13 |
NC_000003.12 |
3 |
126187421 |
- |
ALDH1L1-AS2 |
NNNGAGAAGGAAAGCCACAA |
NGG |
2 |
0.2747 |
Tier III |
| 14 |
NC_000005.10 |
5 |
128719018 |
- |
LOC105379168 |
NNNGAGAAGGACCTCAACAA |
NGG |
2 |
0.2667 |
Tier III |
| 15 |
NC_000001.11 |
1 |
216489912 |
+ |
LOC107985596 |
NNNGAGAAGGACCTCCTCAA |
NGG |
2 |
0.0356 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
45005448 |
- |
Cd3e |
NNNGAGAGGGACCACCACAA |
NGG |
2 |
0.55 |
Tier II |
| 2 |
NC_000068.7 |
2 |
145945991 |
+ |
Cfap61 |
NNNGAGAAGGACCGCCACAC |
NGG |
1 |
0.2273 |
Tier II |
| 3 |
NC_000076.6 |
10 |
20021393 |
- |
Map3k5 |
NNNGAGAAGGACTTCCACAA |
NGG |
2 |
0.1867 |
Tier II |
| 4 |
NC_000074.6 |
8 |
105430170 |
+ |
Lrrc36 |
NNNGAGAAGCAGCGCCACAA |
NGG |
2 |
0.1778 |
Tier II |
| 5 |
NC_000081.6 |
15 |
48278912 |
+ |
Csmd3 |
NNNGAGAAGGAGAGCCACAA |
NGG |
2 |
0.1709 |
Tier II |
| 6 |
NC_000081.6 |
15 |
73629422 |
- |
Slc45a4 |
NNNGAGAAGGCCAGCCACAA |
NGG |
2 |
0.1538 |
Tier II |
| 7 |
NC_000070.6 |
4 |
151069420 |
- |
Camta1 |
NNNCAGAAGGACCTCCACAA |
NGG |
2 |
0.1412 |
Tier II |
| 8 |
NC_000083.6 |
17 |
31599013 |
- |
Pknox1 |
NNNGAGAAGGACCAGCACAA |
NGG |
2 |
0.0375 |
Tier II |
| 9 |
NC_000082.6 |
16 |
95422717 |
- |
Erg |
NNNGAGAAGGACGGCCACAA |
NAG |
2 |
0.0354 |
Tier II |
| 10 |
NC_000077.6 |
11 |
46280757 |
+ |
Cyfip2 |
NNNGAGAAGGACCGGGACAA |
NGG |
2 |
0.0077 |
Tier II |
| 11 |
NC_000070.6 |
4 |
120260011 |
- |
Gm36134 |
NNNGAGAAGGACCCTCACAA |
NGG |
2 |
0.0286 |
Tier III |
| 12 |
NC_000085.6 |
19 |
45544702 |
- |
Gm19639 |
NNNGAGAAGGACGGTCACAA |
NGG |
2 |
0.0091 |
Tier III |
| 13 |
NC_000085.6 |
19 |
45544702 |
- |
Gm41856 |
NNNGAGAAGGACGGTCACAA |
NGG |
2 |
0.0091 |
Tier III |
Other clones with same target sequence:
(none)
