Construct: sgRNA BRDN0001146626
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCTGAGGATCTATACCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPA (8476)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75747
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
227035522 |
+ |
CDC42BPA |
NNNTGAGGATCTATACCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
112145637 |
+ |
TRAFD1 |
NNNTGAGGAGCTCTACCCAG |
NGG |
2 |
0.1053 |
Tier I |
| 3 |
NC_000003.12 |
3 |
4407919 |
+ |
SUMF1 |
NNNTGAAGAACTATACCCAG |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000007.14 |
7 |
48030774 |
+ |
SUN3 |
NNNTAAGGATATATACCCAG |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000015.10 |
15 |
66527012 |
- |
ZWILCH |
NNNTCAGGATATATACCCAG |
NGG |
2 |
0.5893 |
Tier II |
| 6 |
NC_000007.14 |
7 |
105572544 |
+ |
EFCAB10 |
NNNTGTGGATATATACCCAG |
NGG |
2 |
0.5357 |
Tier II |
| 7 |
NC_000004.12 |
4 |
113673550 |
+ |
CAMK2D |
NNNAGAGGAACTATACCCAG |
NGG |
2 |
0.4082 |
Tier II |
| 8 |
NC_000013.11 |
13 |
52695221 |
+ |
SUGT1 |
NNNTGAGGATATATATCCAG |
NGG |
2 |
0.2308 |
Tier II |
| 9 |
NC_000001.11 |
1 |
54180716 |
- |
CYB5RL |
NNNTGGGGATTTATACCCAG |
NGG |
2 |
0.2198 |
Tier II |
| 10 |
NC_000005.10 |
5 |
159085906 |
- |
EBF1 |
NNNTGAGGATCTATACACAC |
NGG |
2 |
0.2 |
Tier II |
| 11 |
NC_000001.11 |
1 |
94456764 |
+ |
ABCD3 |
NNNTTAGGATCTATACCCAG |
NAG |
2 |
0.0778 |
Tier II |
| 12 |
NC_000004.12 |
4 |
62201106 |
+ |
LOC101927145 |
NNNTGAGGAAATATACCCAG |
NGG |
2 |
0.6429 |
Tier III |
| 13 |
NC_000001.11 |
1 |
171133170 |
- |
LOC105371611 |
NNNTGTGGATATATACCCAG |
NGG |
2 |
0.5357 |
Tier III |
| 14 |
NC_000007.14 |
7 |
105572544 |
+ |
EFCAB10-AS1 |
NNNTGTGGATATATACCCAG |
NGG |
2 |
0.5357 |
Tier III |
| 15 |
NC_000006.12 |
6 |
90686425 |
- |
LOC107986623 |
NNNTGGGGATCTATACCCAG |
NAG |
2 |
0.1852 |
Tier III |
| 16 |
NC_000003.12 |
3 |
5359643 |
+ |
LOC124906205 |
NNNTGAGGTTCTAGACCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
121375822 |
- |
Trafd1 |
NNNTGAGGAGCTGTACCCAG |
NGG |
2 |
0.3261 |
Tier I |
| 2 |
NC_000069.6 |
3 |
123109373 |
- |
Synpo2 |
NNNTGAAGATCTATACCCAG |
NGG |
1 |
1.0 |
Tier II |
| 3 |
NC_000075.6 |
9 |
123609457 |
+ |
Slc6a20b |
NNNTGAAGATCCATACCCAG |
NGG |
2 |
0.9474 |
Tier II |
| 4 |
NC_000077.6 |
11 |
55413190 |
+ |
Sparc |
NNNTGAGTATCTAAACCCAG |
NGG |
2 |
0.3869 |
Tier II |
| 5 |
NC_000074.6 |
8 |
93061386 |
- |
Ces1b |
NNNTGAGGACCTATACCCAT |
NGG |
2 |
0.3733 |
Tier II |
| 6 |
NC_000076.6 |
10 |
36748378 |
+ |
Hs3st5 |
NNNTGAGGAGCTATACCCAT |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000071.6 |
5 |
125763214 |
+ |
Tmem132b |
NNNTGAGGATTTATAACCAG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000086.7 |
X |
73896722 |
+ |
Arhgap4 |
NNNTGAAGATCTATACCCAG |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000082.6 |
16 |
52332570 |
+ |
Alcam |
NNNTCAGGATCTATACCCAG |
NAG |
2 |
0.2037 |
Tier II |
| 10 |
NC_000086.7 |
X |
166339654 |
- |
Gpm6b |
NNNGGAGGATCTATACCCAG |
NAG |
2 |
0.162 |
Tier II |
| 11 |
NC_000067.6 |
1 |
126716759 |
+ |
Nckap5 |
NNNTGAGGATGTATACCCAC |
NGG |
2 |
0.1071 |
Tier II |
| 12 |
NC_000071.6 |
5 |
110978394 |
- |
Ttc28 |
NNNTGAGGACCTATTCCCAG |
NGG |
2 |
0.1067 |
Tier II |
| 13 |
NC_000069.6 |
3 |
22115159 |
+ |
Tbl1xr1 |
NNNTTAGGATCTATTCCCAG |
NGG |
2 |
0.06 |
Tier II |
| 14 |
NC_000077.6 |
11 |
66228958 |
+ |
Shisa6 |
NNNTGAGGATCTATAGCCAG |
NAG |
2 |
0.0399 |
Tier II |
| 15 |
NC_000079.6 |
13 |
16986693 |
- |
Sugct |
NNNTGAGGAACTATACCCAG |
NTG |
2 |
0.0334 |
Tier II |
| 16 |
NC_000071.6 |
5 |
130473972 |
- |
Caln1 |
NNNTGAGGATCTATACCCAA |
NGT |
2 |
0.0151 |
Tier II |
| 17 |
NC_000082.6 |
16 |
11618963 |
- |
Snx29 |
NNNTGATGATCTATACCCAG |
NGC |
2 |
0.0127 |
Tier II |
| 18 |
NC_000071.6 |
5 |
125763214 |
+ |
Gm32811 |
NNNTGAGGATTTATAACCAG |
NGG |
2 |
0.3077 |
Tier III |
| 19 |
NC_000082.6 |
16 |
52332570 |
+ |
Gm32597 |
NNNTCAGGATCTATACCCAG |
NAG |
2 |
0.2037 |
Tier III |
| 20 |
NC_000077.6 |
11 |
66228958 |
+ |
Gm12296 |
NNNTGAGGATCTATAGCCAG |
NAG |
2 |
0.0399 |
Tier III |
Other clones with same target sequence:
(none)
