Construct: sgRNA BRDN0001146627
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTCAACATCACAACCATA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ABL2 (27)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77178
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
179131462 |
+ |
ABL2 |
NNNTCAACATCACAACCATA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
119960917 |
- |
PTPN4 |
NNNTCAACATTACAACCATA |
NAG |
2 |
0.0798 |
Tier I |
| 3 |
NC_000002.12 |
2 |
197493160 |
+ |
HSPD1 |
NNNTCAAAAACACAACCATA |
NGG |
2 |
0.5571 |
Tier II |
| 4 |
NC_000004.12 |
4 |
64380144 |
- |
TECRL |
NNNTCAACATCACTAACATA |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000023.11 |
X |
9911839 |
- |
SHROOM2 |
NNNTCAACTCCACAACCATA |
NGG |
2 |
0.32 |
Tier II |
| 6 |
NC_000002.12 |
2 |
161810630 |
- |
SLC4A10 |
NNNTCAAAATCAAAACCATA |
NGG |
2 |
0.25 |
Tier II |
| 7 |
NC_000003.12 |
3 |
73598942 |
+ |
PDZRN3 |
NNNTCACCATCACAACAATA |
NGG |
2 |
0.2042 |
Tier II |
| 8 |
NC_000006.12 |
6 |
17621133 |
+ |
NUP153 |
NNNTCAACATCACTATCATA |
NGG |
2 |
0.1641 |
Tier II |
| 9 |
NC_000020.11 |
20 |
59650206 |
- |
PHACTR3 |
NNNTCAACATCACACCCATT |
NGG |
2 |
0.1636 |
Tier II |
| 10 |
NC_000004.12 |
4 |
42449656 |
+ |
ATP8A1 |
NNNTCAACATCAAAACCATA |
NAG |
2 |
0.0997 |
Tier II |
| 11 |
NC_000015.10 |
15 |
82215728 |
+ |
EFL1 |
NNNTTAACATCACAAGCATA |
NGG |
2 |
0.0979 |
Tier II |
| 12 |
NC_000007.14 |
7 |
832858 |
- |
SUN1 |
NNNTCAACATCACAACCACC |
NGG |
2 |
0.0627 |
Tier II |
| 13 |
NC_000022.11 |
22 |
28636620 |
- |
TTC28 |
NNNTCAACATCACTACCATA |
NGA |
2 |
0.037 |
Tier II |
| 14 |
NC_000023.11 |
X |
71373572 |
- |
TAF1 |
NNNTCAACATCACATCCATA |
NGC |
2 |
0.0044 |
Tier II |
| 15 |
NC_000006.12 |
6 |
90893990 |
+ |
LOC107986623 |
NNNTAAACATCAAAACCATA |
NGG |
2 |
0.2198 |
Tier III |
| 16 |
NC_000006.12 |
6 |
156210085 |
- |
LOC101928923 |
NNNTCAACATCACTACCATC |
NGG |
2 |
0.1212 |
Tier III |
| 17 |
NC_000015.10 |
15 |
84125586 |
- |
EFL1P1 |
NNNTTAACATCACAAGCATA |
NGG |
2 |
0.0979 |
Tier III |
| 18 |
NC_000013.11 |
13 |
48033935 |
+ |
LOC124903172 |
NNNTCAACATCACAAGCATC |
NGG |
2 |
0.035 |
Tier III |
| 19 |
NC_000021.9 |
21 |
16094992 |
+ |
VDAC2P1 |
NNNTCAACATCACAACCAAA |
NTG |
2 |
0.0111 |
Tier III |
| 20 |
NC_000021.9 |
21 |
16094992 |
+ |
MIR99AHG |
NNNTCAACATCACAACCAAA |
NTG |
2 |
0.0111 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
119680086 |
+ |
Ptpn4 |
NNNTCAACATCACAACCATG |
NAG |
2 |
0.1983 |
Tier I |
| 2 |
NC_000079.6 |
13 |
66979556 |
- |
Ptdss1 |
NNNTCAACACCACAACCATG |
NGG |
2 |
0.4078 |
Tier II |
| 3 |
NC_000078.6 |
12 |
4845797 |
- |
Wdcp |
NNNTGAACACCACAACCATA |
NGG |
2 |
0.32 |
Tier II |
| 4 |
NC_000084.6 |
18 |
64900662 |
- |
Nedd4l |
NNNTCAAGATCACAACCACA |
NGG |
2 |
0.1773 |
Tier II |
| 5 |
NC_000075.6 |
9 |
27147065 |
+ |
Jam3 |
NNNTCAACTTCACAACCAGA |
NGG |
2 |
0.15 |
Tier II |
| 6 |
NC_000071.6 |
5 |
30893779 |
- |
Agbl5 |
NNNACAACATCACAACCATC |
NGG |
2 |
0.1082 |
Tier II |
| 7 |
NC_000074.6 |
8 |
119261374 |
- |
Cdh13 |
NNNTCAACATCACATCCACA |
NGG |
2 |
0.0552 |
Tier II |
| 8 |
NC_000067.6 |
1 |
130785766 |
+ |
Fcamr |
NNNTCAACAACACAACCATA |
NGT |
2 |
0.0138 |
Tier II |
| 9 |
NC_000080.6 |
14 |
115456495 |
- |
Gpc5 |
NNNTCAACATCACAAGCATA |
NTG |
2 |
0.006 |
Tier II |
| 10 |
NC_000079.6 |
13 |
44098011 |
- |
Gm33630 |
NNNTCAACATCACAACCATT |
NGG |
1 |
0.6 |
Tier III |
| 11 |
NC_000075.6 |
9 |
27147065 |
+ |
LOC115487155 |
NNNTCAACTTCACAACCAGA |
NGG |
2 |
0.15 |
Tier III |
| 12 |
NC_000074.6 |
8 |
50915607 |
+ |
1700019L22Rik |
NNNTCAACATCACCACCAGA |
NGG |
2 |
0.0536 |
Tier III |
Other clones with same target sequence:
(none)
