Construct: sgRNA BRDN0001146629

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCGGTTCAGATAATCAAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76679

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 51984059 - ACVR1B NNNGTTCAGATAATCAAACA NGG 0 1.0 Tier I
2 NC_000001.11 1 213061842 - RPS6KC1 NNNGTACAAATAATCAAACA NGG 2 0.5571 Tier II
3 NC_000001.11 1 64578070 - CACHD1 NNNGTTAACATAATCAAACA NGG 2 0.4038 Tier II
4 NC_000006.12 6 157674898 - ZDHHC14 NNNGTTCAGAAAATCAAAAA NGG 2 0.3214 Tier II
5 NC_000001.11 1 242193600 - PLD5 NNNGTTCTGATGATCAAACA NGG 2 0.3095 Tier II
6 NC_000008.11 8 140279075 - TRAPPC9 NNNGGTCAGATAATCAAGCA NGG 2 0.256 Tier II
7 NC_000017.11 17 9913027 + GAS7 NNNGATCAGATAATCAATCA NGG 2 0.25 Tier II
8 NC_000014.9 14 79396415 - NRXN3 NNNGTTCTCATAATCAAACA NGG 2 0.2308 Tier II
9 NC_000002.12 2 151728314 + NEB NNNGTTAAGATAATCAAACA NAG 2 0.1944 Tier II
10 NC_000003.12 3 99964044 - FILIP1L NNNGTTCAAATATTCAAACA NGG 2 0.1929 Tier II
11 NC_000003.12 3 99964044 - CMSS1 NNNGTTCAAATATTCAAACA NGG 2 0.1929 Tier II
12 NC_000002.12 2 23394504 + KLHL29 NNNGTTCAGATCAACAAACA NGG 2 0.1629 Tier II
13 NC_000002.12 2 40231051 - SLC8A1 NNNGTTCAGAGAATCAAGCA NGG 2 0.16 Tier II
14 NC_000012.12 12 41223445 + PDZRN4 NNNGTTCAGATAATCACACA NAG 2 0.0458 Tier II
15 NC_000001.11 1 98701360 - SNX7 NNNGATCAGATAATCAAACA NGA 2 0.0347 Tier II
16 NC_000011.10 11 77104215 + CAPN5 NNNGTTCAGATAGTGAAACA NGG 2 0.0326 Tier II
17 NC_000014.9 14 33185388 + NPAS3 NNNGTTCAGATAATGAAAAA NGG 2 0.0214 Tier II
18 NC_000010.11 10 72524787 - MICU1 NNNGTTCATATAATCAAACA NTG 2 0.0208 Tier II
19 NC_000003.12 3 136433114 - STAG1 NNNGTTCAGATAATCATAGA NGG 2 0.0167 Tier II
20 NC_000006.12 6 161667924 + PRKN NNNTTTCAGATAATCAAACA NTG 2 0.0142 Tier II
21 NC_000004.12 4 92706711 - GRID2 NNNGTTCTGATAATCAAACA NGT 2 0.0069 Tier II
22 NC_000015.10 15 52212402 + MYO5C NNNGTTAAGATAAGCAAACA NGG 2 0.0 Tier II
23 NC_000018.10 18 4369085 + DLGAP1 NNNGTTCAGATAAGCAAAGA NGG 2 0.0 Tier II
24 NC_000001.11 1 64578070 - LOC124904195 NNNGTTAACATAATCAAACA NGG 2 0.4038 Tier III
25 NC_000003.12 3 34558336 + LOC102724048 NNNGTTCAAATAATCAAAAA NGG 2 0.2755 Tier III
26 NC_000016.10 16 64465550 - LOC105371310 NNNGGTCTGATAATCAAACA NGG 2 0.2743 Tier III
27 NC_000005.10 5 92676298 - LOC105379082 NNNGGTCAGAGAATCAAACA NGG 2 0.256 Tier III
28 NC_000008.11 8 140279075 - LOC105375779 NNNGGTCAGATAATCAAGCA NGG 2 0.256 Tier III
29 NC_000003.12 3 99964044 - LOC105374010 NNNGTTCAAATATTCAAACA NGG 2 0.1929 Tier III
30 NC_000002.12 2 40231051 - SLC8A1-AS1 NNNGTTCAGAGAATCAAGCA NGG 2 0.16 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 101202038 - Acvr1b NNNGTTCAGATAATCAAACA NTG 1 0.039 Tier I
2 NC_000084.6 18 56414276 - Gramd3 NNNGTTCAGACAATCAAACA NGG 1 0.6667 Tier II
3 NC_000070.6 4 123457492 + Macf1 NNNGTTCTGATGATCAAACA NGG 2 0.3095 Tier II
4 NC_000070.6 4 55351444 - Rad23b NNNTTTCAGAAAATCAAACA NGG 2 0.2727 Tier II
5 NC_000071.6 5 81557988 + Adgrl3 NNNGTGCTGATAATCAAACA NGG 2 0.2449 Tier II
6 NC_000069.6 3 13980236 + Ralyl NNNGTTCAGATAATAAAAAA NGG 2 0.0952 Tier II
7 NC_000068.7 2 106225421 + Dcdc5 NNNGTTCAGAGAATCAAACC NGG 2 0.0909 Tier II
8 NC_000076.6 10 45379479 - Lin28b NNNGCTCAGATAATCAAACA NGA 2 0.0694 Tier II
9 NC_000086.7 X 143565157 - Pak3 NNNGTTCAGATAGTCAAACA NGA 2 0.0453 Tier II
10 NC_000067.6 1 95325972 - Fam174a NNNGTTCAGATAAATAAACA NGG 2 0.0413 Tier II
11 NC_000081.6 15 41624760 - Oxr1 NNNTTTCAGATAATTAAACA NGG 2 0.0242 Tier II
12 NC_000071.6 5 13398287 + Sema3a NNNGTTCAGATCATCAAACA NGA 2 0.0183 Tier II
13 NC_000074.6 8 78816726 + Lsm6 NNNGTTCAGATACTTAAACA NGG 2 0.014 Tier II
14 NC_000071.6 5 121002103 - Rph3a NNNGTTCAGCTAATCAAACA NTG 2 0.013 Tier II
15 NC_000081.6 15 47616007 + Csmd3 NNNGTTCAGCTAATCAAACA NTG 2 0.013 Tier II
16 NC_000078.6 12 55306169 - Prorp NNNGTTAAGATAATCAAACA NGT 2 0.0121 Tier II
17 NC_000081.6 15 26815565 - Fbxl7 NNNGTTCAGATTATCAAACA NGT 2 0.0054 Tier II
18 NC_000075.6 9 53323654 + Exph5 NNNGTTCAGATAAGCAAGCA NGG 2 0.0 Tier II
19 NC_000084.6 18 56414276 - LOC102637792 NNNGTTCAGACAATCAAACA NGG 1 0.6667 Tier III
20 NC_000080.6 14 109492024 + Gm30216 NNNGGTCAGAAAATCAAACA NGG 2 0.48 Tier III
21 NC_000074.6 8 96346607 + Gm32005 NNNGTTCAGAGAATCAAACC NGG 2 0.0909 Tier III
22 NC_000072.6 6 31299369 + 2210408F21Rik NNNGTTCTGATAATCAAACA NGT 2 0.0069 Tier III
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Other clones with same target sequence:

(none)