Construct: sgRNA BRDN0001146629
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGTTCAGATAATCAAACA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ACVR1B (91)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000012.12 | 12 | 51984059 | - | ACVR1B | NNNGTTCAGATAATCAAACA | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 213061842 | - | RPS6KC1 | NNNGTACAAATAATCAAACA | NGG | 2 | 0.5571 | Tier II |
3 | NC_000001.11 | 1 | 64578070 | - | CACHD1 | NNNGTTAACATAATCAAACA | NGG | 2 | 0.4038 | Tier II |
4 | NC_000006.12 | 6 | 157674898 | - | ZDHHC14 | NNNGTTCAGAAAATCAAAAA | NGG | 2 | 0.3214 | Tier II |
5 | NC_000001.11 | 1 | 242193600 | - | PLD5 | NNNGTTCTGATGATCAAACA | NGG | 2 | 0.3095 | Tier II |
6 | NC_000008.11 | 8 | 140279075 | - | TRAPPC9 | NNNGGTCAGATAATCAAGCA | NGG | 2 | 0.256 | Tier II |
7 | NC_000017.11 | 17 | 9913027 | + | GAS7 | NNNGATCAGATAATCAATCA | NGG | 2 | 0.25 | Tier II |
8 | NC_000014.9 | 14 | 79396415 | - | NRXN3 | NNNGTTCTCATAATCAAACA | NGG | 2 | 0.2308 | Tier II |
9 | NC_000002.12 | 2 | 151728314 | + | NEB | NNNGTTAAGATAATCAAACA | NAG | 2 | 0.1944 | Tier II |
10 | NC_000003.12 | 3 | 99964044 | - | FILIP1L | NNNGTTCAAATATTCAAACA | NGG | 2 | 0.1929 | Tier II |
11 | NC_000003.12 | 3 | 99964044 | - | CMSS1 | NNNGTTCAAATATTCAAACA | NGG | 2 | 0.1929 | Tier II |
12 | NC_000002.12 | 2 | 23394504 | + | KLHL29 | NNNGTTCAGATCAACAAACA | NGG | 2 | 0.1629 | Tier II |
13 | NC_000002.12 | 2 | 40231051 | - | SLC8A1 | NNNGTTCAGAGAATCAAGCA | NGG | 2 | 0.16 | Tier II |
14 | NC_000012.12 | 12 | 41223445 | + | PDZRN4 | NNNGTTCAGATAATCACACA | NAG | 2 | 0.0458 | Tier II |
15 | NC_000001.11 | 1 | 98701360 | - | SNX7 | NNNGATCAGATAATCAAACA | NGA | 2 | 0.0347 | Tier II |
16 | NC_000011.10 | 11 | 77104215 | + | CAPN5 | NNNGTTCAGATAGTGAAACA | NGG | 2 | 0.0326 | Tier II |
17 | NC_000014.9 | 14 | 33185388 | + | NPAS3 | NNNGTTCAGATAATGAAAAA | NGG | 2 | 0.0214 | Tier II |
18 | NC_000010.11 | 10 | 72524787 | - | MICU1 | NNNGTTCATATAATCAAACA | NTG | 2 | 0.0208 | Tier II |
19 | NC_000003.12 | 3 | 136433114 | - | STAG1 | NNNGTTCAGATAATCATAGA | NGG | 2 | 0.0167 | Tier II |
20 | NC_000006.12 | 6 | 161667924 | + | PRKN | NNNTTTCAGATAATCAAACA | NTG | 2 | 0.0142 | Tier II |
21 | NC_000004.12 | 4 | 92706711 | - | GRID2 | NNNGTTCTGATAATCAAACA | NGT | 2 | 0.0069 | Tier II |
22 | NC_000015.10 | 15 | 52212402 | + | MYO5C | NNNGTTAAGATAAGCAAACA | NGG | 2 | 0.0 | Tier II |
23 | NC_000018.10 | 18 | 4369085 | + | DLGAP1 | NNNGTTCAGATAAGCAAAGA | NGG | 2 | 0.0 | Tier II |
24 | NC_000001.11 | 1 | 64578070 | - | LOC124904195 | NNNGTTAACATAATCAAACA | NGG | 2 | 0.4038 | Tier III |
25 | NC_000003.12 | 3 | 34558336 | + | LOC102724048 | NNNGTTCAAATAATCAAAAA | NGG | 2 | 0.2755 | Tier III |
26 | NC_000016.10 | 16 | 64465550 | - | LOC105371310 | NNNGGTCTGATAATCAAACA | NGG | 2 | 0.2743 | Tier III |
27 | NC_000005.10 | 5 | 92676298 | - | LOC105379082 | NNNGGTCAGAGAATCAAACA | NGG | 2 | 0.256 | Tier III |
28 | NC_000008.11 | 8 | 140279075 | - | LOC105375779 | NNNGGTCAGATAATCAAGCA | NGG | 2 | 0.256 | Tier III |
29 | NC_000003.12 | 3 | 99964044 | - | LOC105374010 | NNNGTTCAAATATTCAAACA | NGG | 2 | 0.1929 | Tier III |
30 | NC_000002.12 | 2 | 40231051 | - | SLC8A1-AS1 | NNNGTTCAGAGAATCAAGCA | NGG | 2 | 0.16 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000081.6 | 15 | 101202038 | - | Acvr1b | NNNGTTCAGATAATCAAACA | NTG | 1 | 0.039 | Tier I |
2 | NC_000084.6 | 18 | 56414276 | - | Gramd3 | NNNGTTCAGACAATCAAACA | NGG | 1 | 0.6667 | Tier II |
3 | NC_000070.6 | 4 | 123457492 | + | Macf1 | NNNGTTCTGATGATCAAACA | NGG | 2 | 0.3095 | Tier II |
4 | NC_000070.6 | 4 | 55351444 | - | Rad23b | NNNTTTCAGAAAATCAAACA | NGG | 2 | 0.2727 | Tier II |
5 | NC_000071.6 | 5 | 81557988 | + | Adgrl3 | NNNGTGCTGATAATCAAACA | NGG | 2 | 0.2449 | Tier II |
6 | NC_000069.6 | 3 | 13980236 | + | Ralyl | NNNGTTCAGATAATAAAAAA | NGG | 2 | 0.0952 | Tier II |
7 | NC_000068.7 | 2 | 106225421 | + | Dcdc5 | NNNGTTCAGAGAATCAAACC | NGG | 2 | 0.0909 | Tier II |
8 | NC_000076.6 | 10 | 45379479 | - | Lin28b | NNNGCTCAGATAATCAAACA | NGA | 2 | 0.0694 | Tier II |
9 | NC_000086.7 | X | 143565157 | - | Pak3 | NNNGTTCAGATAGTCAAACA | NGA | 2 | 0.0453 | Tier II |
10 | NC_000067.6 | 1 | 95325972 | - | Fam174a | NNNGTTCAGATAAATAAACA | NGG | 2 | 0.0413 | Tier II |
11 | NC_000081.6 | 15 | 41624760 | - | Oxr1 | NNNTTTCAGATAATTAAACA | NGG | 2 | 0.0242 | Tier II |
12 | NC_000071.6 | 5 | 13398287 | + | Sema3a | NNNGTTCAGATCATCAAACA | NGA | 2 | 0.0183 | Tier II |
13 | NC_000074.6 | 8 | 78816726 | + | Lsm6 | NNNGTTCAGATACTTAAACA | NGG | 2 | 0.014 | Tier II |
14 | NC_000071.6 | 5 | 121002103 | - | Rph3a | NNNGTTCAGCTAATCAAACA | NTG | 2 | 0.013 | Tier II |
15 | NC_000081.6 | 15 | 47616007 | + | Csmd3 | NNNGTTCAGCTAATCAAACA | NTG | 2 | 0.013 | Tier II |
16 | NC_000078.6 | 12 | 55306169 | - | Prorp | NNNGTTAAGATAATCAAACA | NGT | 2 | 0.0121 | Tier II |
17 | NC_000081.6 | 15 | 26815565 | - | Fbxl7 | NNNGTTCAGATTATCAAACA | NGT | 2 | 0.0054 | Tier II |
18 | NC_000075.6 | 9 | 53323654 | + | Exph5 | NNNGTTCAGATAAGCAAGCA | NGG | 2 | 0.0 | Tier II |
19 | NC_000084.6 | 18 | 56414276 | - | LOC102637792 | NNNGTTCAGACAATCAAACA | NGG | 1 | 0.6667 | Tier III |
20 | NC_000080.6 | 14 | 109492024 | + | Gm30216 | NNNGGTCAGAAAATCAAACA | NGG | 2 | 0.48 | Tier III |
21 | NC_000074.6 | 8 | 96346607 | + | Gm32005 | NNNGTTCAGAGAATCAAACC | NGG | 2 | 0.0909 | Tier III |
22 | NC_000072.6 | 6 | 31299369 | + | 2210408F21Rik | NNNGTTCTGATAATCAAACA | NGT | 2 | 0.0069 | Tier III |