Construct: sgRNA BRDN0001146632
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGGACCACAGGTCGTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTBK1 (84630)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75802
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43255569 |
- |
TTBK1 |
NNNGGGACCACAGGTCGTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
45152791 |
+ |
NKPD1 |
NNNGGTACAACAGGTCGTCG |
NGG |
2 |
0.5714 |
Tier I |
| 3 |
NC_000017.11 |
17 |
39668717 |
+ |
PNMT |
NNNGGGACAAGAGGTCGTCG |
NGG |
2 |
0.2143 |
Tier II |
| 4 |
NC_000011.10 |
11 |
809448 |
+ |
PIDD1 |
NNNGGGACCACCGGTCGCCG |
NGG |
2 |
0.1128 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46476996 |
+ |
Ttbk1 |
NNNGGGACCACAGGTCATCA |
NGG |
2 |
0.875 |
Tier I |
| 2 |
NC_000071.6 |
5 |
131712686 |
+ |
Auts2 |
NNNGGGACCACAGGTAGTTG |
NGG |
2 |
0.4615 |
Tier II |
| 3 |
NC_000073.6 |
7 |
44316251 |
+ |
Shank1 |
NNNGGGACCAAAGGTTGTCG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000075.6 |
9 |
112234775 |
- |
Arpp21 |
NNNGGGACCACAGGTCGCAG |
NGG |
2 |
0.1837 |
Tier II |
| 5 |
NC_000085.6 |
19 |
4001160 |
- |
Nudt8 |
NNNGGGACCACAGGTCGTCA |
NGA |
2 |
0.0651 |
Tier II |
| 6 |
NC_000075.6 |
9 |
112234775 |
- |
2900079G21Rik |
NNNGGGACCACAGGTCGCAG |
NGG |
2 |
0.1837 |
Tier III |
Other clones with same target sequence:
(none)
