Construct: sgRNA BRDN0001146637
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTTGCTAATGCTACCACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSF1R (1436)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77038
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150073317 |
- |
CSF1R |
NNNTGCTAATGCTACCACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
80038157 |
- |
TPD52 |
NNNTGCTAGTGCCACCACCA |
NGG |
2 |
0.5263 |
Tier I |
| 3 |
NC_000004.12 |
4 |
163325653 |
+ |
NPY1R |
NNNTGCAAATGCTACCACAA |
NGG |
2 |
0.375 |
Tier I |
| 4 |
NC_000019.10 |
19 |
6663957 |
- |
TNFSF14 |
NNNTGTAAATGCTACCACCA |
NGG |
2 |
0.8125 |
Tier II |
| 5 |
NC_000017.11 |
17 |
12894511 |
- |
ARHGAP44 |
NNNTGCAAATGCCACCACCA |
NGG |
2 |
0.6908 |
Tier II |
| 6 |
NC_000003.12 |
3 |
96894506 |
+ |
EPHA6 |
NNNTGCTATTGCTACAACCA |
NGG |
2 |
0.6 |
Tier II |
| 7 |
NC_000008.11 |
8 |
80038157 |
- |
TPD52-MRPS28 |
NNNTGCTAGTGCCACCACCA |
NGG |
2 |
0.5263 |
Tier II |
| 8 |
NC_000010.11 |
10 |
62399402 |
- |
ZNF365 |
NNNTGATAATGCTACCACAA |
NGG |
2 |
0.398 |
Tier II |
| 9 |
NC_000003.12 |
3 |
127978672 |
- |
KBTBD12 |
NNNTGCTATTGCTACCACCT |
NGG |
2 |
0.36 |
Tier II |
| 10 |
NC_000003.12 |
3 |
7185152 |
+ |
GRM7 |
NNNTGCAAATGCGACCACCA |
NGG |
2 |
0.2283 |
Tier II |
| 11 |
NC_000003.12 |
3 |
60584232 |
- |
FHIT |
NNNTGCTGATGCTCCCACCA |
NGG |
2 |
0.1571 |
Tier II |
| 12 |
NC_000003.12 |
3 |
183202029 |
- |
MCF2L2 |
NNNTTCTAAGGCTACCACCA |
NGG |
2 |
0.15 |
Tier II |
| 13 |
NC_000014.9 |
14 |
71466207 |
+ |
SIPA1L1 |
NNNAGCTAATGCTACCACCA |
NAG |
2 |
0.1235 |
Tier II |
| 14 |
NC_000010.11 |
10 |
17840230 |
- |
MRC1 |
NNNTGCTTATGCTAACACCA |
NGG |
2 |
0.0952 |
Tier II |
| 15 |
NC_000006.12 |
6 |
41282339 |
+ |
TREM1 |
NNNTGCTGATGCTACCACCA |
NTG |
2 |
0.0286 |
Tier II |
| 16 |
NC_000001.11 |
1 |
211827744 |
+ |
LPGAT1 |
NNNTGCTATTGCTACCACCA |
NGT |
2 |
0.0097 |
Tier II |
| 17 |
NC_000001.11 |
1 |
15614723 |
- |
CD24P1 |
NNNAGCTAATGCCACCACCA |
NGG |
2 |
0.3759 |
Tier III |
| 18 |
NC_000007.14 |
7 |
150876229 |
+ |
LOC105375567 |
NNNTCCTAATGCTACCACTA |
NGG |
2 |
0.3626 |
Tier III |
| 19 |
NC_000001.11 |
1 |
34524634 |
+ |
LOC105378641 |
NNNTGGTAATGCTACCACCA |
NGC |
2 |
0.0111 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
14932599 |
- |
Gm11837 |
NNNTGCCAATGCTACCACCA |
NGG |
1 |
0.6875 |
Tier II |
| 2 |
NC_000073.6 |
7 |
99116424 |
+ |
Uvrag |
NNNTCCTAAAGCTACCACCA |
NGG |
2 |
0.6735 |
Tier II |
| 3 |
NC_000067.6 |
1 |
33918380 |
- |
Dst |
NNNTGCAGATGCTACCACCA |
NGG |
2 |
0.6417 |
Tier II |
| 4 |
NC_000076.6 |
10 |
61893228 |
- |
Col13a1 |
NNNTCCTAATGCTACCATCA |
NGG |
2 |
0.5051 |
Tier II |
| 5 |
NC_000081.6 |
15 |
100104340 |
+ |
Dip2b |
NNNTGCTAGTGCAACCACCA |
NGG |
2 |
0.4615 |
Tier II |
| 6 |
NC_000076.6 |
10 |
61099059 |
+ |
Sgpl1 |
NNNTGCTAGTGCTACCACAA |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000068.7 |
2 |
113819066 |
+ |
Scg5 |
NNNTGCTAATGTTGCCACCA |
NGG |
2 |
0.2513 |
Tier II |
| 8 |
NC_000078.6 |
12 |
116737916 |
+ |
Ptprn2 |
NNNTGCTAATGCTACCAACC |
NGG |
2 |
0.1224 |
Tier II |
| 9 |
NC_000067.6 |
1 |
136138315 |
+ |
Kif21b |
NNNTGCTACTGCTACCCCCA |
NGG |
2 |
0.1008 |
Tier II |
| 10 |
NC_000068.7 |
2 |
106360019 |
- |
Dcdc5 |
NNNTGCTGATGCTACCTCCA |
NGG |
2 |
0.0978 |
Tier II |
| 11 |
NC_000083.6 |
17 |
84227865 |
+ |
Thada |
NNNTGCTAATGCTAACCCCA |
NGG |
2 |
0.0392 |
Tier II |
| 12 |
NC_000067.6 |
1 |
178853445 |
- |
Kif26b |
NNNTGCTTATGCTACCACCA |
NGA |
2 |
0.0298 |
Tier II |
| 13 |
NC_000068.7 |
2 |
52560352 |
+ |
Cacnb4 |
NNNTGCTAATGCTAACACCA |
NGC |
2 |
0.0049 |
Tier II |
| 14 |
NC_000081.6 |
15 |
52901489 |
+ |
Gm41323 |
NNNTGCTAATGCTGCCACCA |
NTG |
2 |
0.0182 |
Tier III |
Other clones with same target sequence:
(none)
