Construct: sgRNA BRDN0001146638
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGGTGTGATCCTATCCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAD (790)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76962
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000002.12 |
2 |
27233362 |
+ |
CAD |
NNNGGTGTGATCCTATCCAT |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000006.12 |
6 |
69144538 |
+ |
ADGRB3 |
NNNAGTATGATCCTATCCAT |
NGG |
2 |
0.9 |
Tier II |
3 |
NC_000003.12 |
3 |
72412768 |
- |
RYBP |
NNNGGACTGATCCTATCCAT |
NGG |
2 |
0.5958 |
Tier II |
4 |
NC_000002.12 |
2 |
54056410 |
+ |
ACYP2 |
NNNGGTTTGATCATATCCAT |
NGG |
2 |
0.2198 |
Tier II |
5 |
NC_000005.10 |
5 |
58834793 |
+ |
RAB3C |
NNNGGTGTGGTCATATCCAT |
NGG |
2 |
0.2137 |
Tier II |
6 |
NC_000015.10 |
15 |
89785607 |
- |
ANPEP |
NNNGGTGTGGTCCCATCCAT |
NGG |
2 |
0.1587 |
Tier II |
7 |
NC_000019.10 |
19 |
1117838 |
+ |
SBNO2 |
NNNGGTGTGATCCTGTCCAG |
NGG |
2 |
0.1147 |
Tier II |
8 |
NC_000011.10 |
11 |
19760060 |
- |
NAV2 |
NNNGGTGTTATCCTATCCAT |
NTG |
2 |
0.0208 |
Tier II |
9 |
NC_000011.10 |
11 |
100167637 |
+ |
CNTN5 |
NNNGGTGTGATCCTATGCAT |
NTG |
2 |
0.0023 |
Tier II |
10 |
NC_000004.12 |
4 |
142448546 |
- |
INPP4B |
NNNGGTGTGATCCTATCCAT |
NCA |
2 |
0.0 |
Tier II |
11 |
NC_000007.14 |
7 |
135305761 |
+ |
SLC23A4P |
NNNGGCGTCATCCTATCCAT |
NGG |
2 |
0.4895 |
Tier III |
12 |
NC_000007.14 |
7 |
135305761 |
+ |
LOC107984123 |
NNNGGCGTCATCCTATCCAT |
NGG |
2 |
0.4895 |
Tier III |
13 |
NC_000016.10 |
16 |
60501682 |
+ |
LOC101927605 |
NNNTTTGTGATCCTATCCAT |
NGG |
2 |
0.1091 |
Tier III |
14 |
NC_000011.10 |
11 |
19760060 |
- |
MIR4694 |
NNNGGTGTTATCCTATCCAT |
NTG |
2 |
0.0208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000071.6 |
5 |
31068413 |
+ |
Cad |
NNNGGTGTGATCCTGTCCAT |
NGG |
1 |
0.65 |
Tier I |
2 |
NC_000086.7 |
X |
150655480 |
+ |
Tro |
NNNGGTGTGGTCTTATCCAT |
NGG |
2 |
0.3889 |
Tier I |
3 |
NC_000081.6 |
15 |
78603253 |
- |
Cyth4 |
NNNGGTGTGATCTTATCCAA |
NGG |
2 |
0.3938 |
Tier II |
4 |
NC_000074.6 |
8 |
17101558 |
- |
Csmd1 |
NNNGGGCTGATCCTATCCAT |
NGG |
2 |
0.3929 |
Tier II |
5 |
NC_000068.7 |
2 |
170023073 |
+ |
Tshz2 |
NNNGGGGTTATCCTATCCAT |
NGG |
2 |
0.3048 |
Tier II |
6 |
NC_000077.6 |
11 |
64010404 |
+ |
Cox10 |
NNNGGTGTGAGCCTGTCCAT |
NGG |
2 |
0.26 |
Tier II |
7 |
NC_000074.6 |
8 |
109795247 |
- |
Ap1g1 |
NNNGGTGTGACCATATCCAT |
NGG |
2 |
0.2564 |
Tier II |
8 |
NC_000074.6 |
8 |
118908713 |
+ |
Cdh13 |
NNNGGTGTGATCCTATCTGT |
NGG |
2 |
0.2411 |
Tier II |
9 |
NC_000070.6 |
4 |
147925864 |
- |
Plod1 |
NNNGGTGTCATGCTATCCAT |
NGG |
2 |
0.2393 |
Tier II |
10 |
NC_000067.6 |
1 |
123785906 |
- |
Dpp10 |
NNNGGTGTGATGCTATTCAT |
NGG |
2 |
0.2074 |
Tier II |
11 |
NC_000067.6 |
1 |
123430659 |
- |
Dpp10 |
NNNGGTGTGAGGCTATCCAT |
NGG |
2 |
0.1778 |
Tier II |
12 |
NC_000075.6 |
9 |
55365773 |
- |
Tmem266 |
NNNGGTGTGATGCTAGCCAT |
NGG |
2 |
0.1538 |
Tier II |
13 |
NC_000068.7 |
2 |
131052596 |
- |
Adam33 |
NNNGGTGTGGTCCTTTCCAT |
NGG |
2 |
0.1111 |
Tier II |
14 |
NC_000071.6 |
5 |
115665580 |
- |
Bicdl1 |
NNNGTTGTGATCCTCTCCAT |
NGG |
2 |
0.0818 |
Tier II |
15 |
NC_000084.6 |
18 |
74598663 |
- |
Myo5b |
NNNGGTGTGAGCCTTTCCAT |
NGG |
2 |
0.08 |
Tier II |
16 |
NC_000078.6 |
12 |
37305738 |
- |
Agmo |
NNNGGTGTGATCCTAGCCCT |
NGG |
2 |
0.0716 |
Tier II |
17 |
NC_000071.6 |
5 |
107141558 |
- |
Tgfbr3 |
NNNGGTGTGATCCTAAGCAT |
NGG |
2 |
0.0535 |
Tier II |
18 |
NC_000081.6 |
15 |
94427816 |
+ |
Adamts20 |
NNNGGTGTGATCCTATCCAC |
NAG |
2 |
0.0236 |
Tier II |
19 |
NC_000085.6 |
19 |
11010888 |
- |
Ms4a18 |
NNNGGTGTGGTCCTATCCAT |
NGC |
2 |
0.0123 |
Tier II |
20 |
NC_000085.6 |
19 |
11010915 |
- |
Ms4a18 |
NNNGGTGTGGTCCTATCCAT |
NGC |
2 |
0.0123 |
Tier II |
21 |
NC_000081.6 |
15 |
78603253 |
- |
Gm52186 |
NNNGGTGTGATCTTATCCAA |
NGG |
2 |
0.3938 |
Tier III |
22 |
NC_000067.6 |
1 |
189491091 |
- |
Gm46177 |
NNNGGTGTGGTCCTCTCCAT |
NGG |
2 |
0.1515 |
Tier III |
23 |
NC_000076.6 |
10 |
110826945 |
+ |
1700020G17Rik |
NNNGGTGTGACCCTATCCAG |
NGG |
2 |
0.1176 |
Tier III |
Other clones with same target sequence:
(none)