Construct: sgRNA BRDN0001146638

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGGTGTGATCCTATCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAD (790)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76962

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27233362 + CAD NNNGGTGTGATCCTATCCAT NGG 0 1.0 Tier I
2 NC_000006.12 6 69144538 + ADGRB3 NNNAGTATGATCCTATCCAT NGG 2 0.9 Tier II
3 NC_000003.12 3 72412768 - RYBP NNNGGACTGATCCTATCCAT NGG 2 0.5958 Tier II
4 NC_000002.12 2 54056410 + ACYP2 NNNGGTTTGATCATATCCAT NGG 2 0.2198 Tier II
5 NC_000005.10 5 58834793 + RAB3C NNNGGTGTGGTCATATCCAT NGG 2 0.2137 Tier II
6 NC_000015.10 15 89785607 - ANPEP NNNGGTGTGGTCCCATCCAT NGG 2 0.1587 Tier II
7 NC_000019.10 19 1117838 + SBNO2 NNNGGTGTGATCCTGTCCAG NGG 2 0.1147 Tier II
8 NC_000011.10 11 19760060 - NAV2 NNNGGTGTTATCCTATCCAT NTG 2 0.0208 Tier II
9 NC_000011.10 11 100167637 + CNTN5 NNNGGTGTGATCCTATGCAT NTG 2 0.0023 Tier II
10 NC_000004.12 4 142448546 - INPP4B NNNGGTGTGATCCTATCCAT NCA 2 0.0 Tier II
11 NC_000007.14 7 135305761 + SLC23A4P NNNGGCGTCATCCTATCCAT NGG 2 0.4895 Tier III
12 NC_000007.14 7 135305761 + LOC107984123 NNNGGCGTCATCCTATCCAT NGG 2 0.4895 Tier III
13 NC_000016.10 16 60501682 + LOC101927605 NNNTTTGTGATCCTATCCAT NGG 2 0.1091 Tier III
14 NC_000011.10 11 19760060 - MIR4694 NNNGGTGTTATCCTATCCAT NTG 2 0.0208 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31068413 + Cad NNNGGTGTGATCCTGTCCAT NGG 1 0.65 Tier I
2 NC_000086.7 X 150655480 + Tro NNNGGTGTGGTCTTATCCAT NGG 2 0.3889 Tier I
3 NC_000081.6 15 78603253 - Cyth4 NNNGGTGTGATCTTATCCAA NGG 2 0.3938 Tier II
4 NC_000074.6 8 17101558 - Csmd1 NNNGGGCTGATCCTATCCAT NGG 2 0.3929 Tier II
5 NC_000068.7 2 170023073 + Tshz2 NNNGGGGTTATCCTATCCAT NGG 2 0.3048 Tier II
6 NC_000077.6 11 64010404 + Cox10 NNNGGTGTGAGCCTGTCCAT NGG 2 0.26 Tier II
7 NC_000074.6 8 109795247 - Ap1g1 NNNGGTGTGACCATATCCAT NGG 2 0.2564 Tier II
8 NC_000074.6 8 118908713 + Cdh13 NNNGGTGTGATCCTATCTGT NGG 2 0.2411 Tier II
9 NC_000070.6 4 147925864 - Plod1 NNNGGTGTCATGCTATCCAT NGG 2 0.2393 Tier II
10 NC_000067.6 1 123785906 - Dpp10 NNNGGTGTGATGCTATTCAT NGG 2 0.2074 Tier II
11 NC_000067.6 1 123430659 - Dpp10 NNNGGTGTGAGGCTATCCAT NGG 2 0.1778 Tier II
12 NC_000075.6 9 55365773 - Tmem266 NNNGGTGTGATGCTAGCCAT NGG 2 0.1538 Tier II
13 NC_000068.7 2 131052596 - Adam33 NNNGGTGTGGTCCTTTCCAT NGG 2 0.1111 Tier II
14 NC_000071.6 5 115665580 - Bicdl1 NNNGTTGTGATCCTCTCCAT NGG 2 0.0818 Tier II
15 NC_000084.6 18 74598663 - Myo5b NNNGGTGTGAGCCTTTCCAT NGG 2 0.08 Tier II
16 NC_000078.6 12 37305738 - Agmo NNNGGTGTGATCCTAGCCCT NGG 2 0.0716 Tier II
17 NC_000071.6 5 107141558 - Tgfbr3 NNNGGTGTGATCCTAAGCAT NGG 2 0.0535 Tier II
18 NC_000081.6 15 94427816 + Adamts20 NNNGGTGTGATCCTATCCAC NAG 2 0.0236 Tier II
19 NC_000085.6 19 11010888 - Ms4a18 NNNGGTGTGGTCCTATCCAT NGC 2 0.0123 Tier II
20 NC_000085.6 19 11010915 - Ms4a18 NNNGGTGTGGTCCTATCCAT NGC 2 0.0123 Tier II
21 NC_000081.6 15 78603253 - Gm52186 NNNGGTGTGATCTTATCCAA NGG 2 0.3938 Tier III
22 NC_000067.6 1 189491091 - Gm46177 NNNGGTGTGGTCCTCTCCAT NGG 2 0.1515 Tier III
23 NC_000076.6 10 110826945 + 1700020G17Rik NNNGGTGTGACCCTATCCAG NGG 2 0.1176 Tier III
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Other clones with same target sequence:

(none)