Construct: sgRNA BRDN0001146642
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGGACAACTGGTAGTCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPKAPK3 (7867)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77239
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
50617690 |
- |
MAPKAPK3 |
NNNGGACAACTGGTAGTCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
124107922 |
+ |
CNTNAP5 |
NNNGGACAACTTGTAGTGGT |
NGG |
2 |
0.0513 |
Tier II |
| 3 |
NC_000012.12 |
12 |
18379804 |
- |
PIK3C2G |
NNNGGCCAACTGGGAGTCGT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000007.14 |
7 |
16969064 |
+ |
LOC124901594 |
NNNGGACAACTGGTTGTCGT |
NAG |
2 |
0.0519 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
107289215 |
+ |
Mapkapk3 |
NNNGGACAACTGGTAGTCAT |
NAG |
2 |
0.1852 |
Tier I |
| 2 |
NC_000076.6 |
10 |
82877501 |
- |
Txnrd1 |
NNNGGACAACTGGTAGCCCT |
NGG |
2 |
0.3164 |
Tier II |
| 3 |
NC_000081.6 |
15 |
81382523 |
+ |
St13 |
NNNGGACAACTGGTAGAAGT |
NGG |
2 |
0.2872 |
Tier II |
| 4 |
NC_000081.6 |
15 |
53424385 |
- |
Samd12 |
NNNGGACTACTGGAAGTCGT |
NGG |
2 |
0.2653 |
Tier II |
| 5 |
NC_000068.7 |
2 |
101833783 |
- |
Prr5l |
NNNGGACAACTTGTAGTAGT |
NGG |
2 |
0.2071 |
Tier II |
| 6 |
NC_000085.6 |
19 |
43453368 |
- |
Cnnm1 |
NNNGGACAACTTGTAGTAGT |
NGG |
2 |
0.2071 |
Tier II |
| 7 |
NC_000072.6 |
6 |
136749463 |
- |
Gucy2c |
NNNGGACAACTGGTAGGAGT |
NGG |
2 |
0.0633 |
Tier II |
| 8 |
NC_000080.6 |
14 |
63399979 |
+ |
Blk |
NNNGGACAACTGGTAGGAGT |
NGG |
2 |
0.0633 |
Tier II |
Other clones with same target sequence:
(none)
