Construct: sgRNA BRDN0001146643
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCTGACACTGATGAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK6 (2870)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75704
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
177433976 |
+ |
GRK6 |
NNNCTGACACTGATGAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
10644202 |
+ |
CELF2 |
NNNCTGACACTGGTGAACAG |
NGG |
2 |
0.4658 |
Tier II |
| 3 |
NC_000008.11 |
8 |
6735232 |
- |
AGPAT5 |
NNNCTGAAACTGATGAACAG |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000020.11 |
20 |
63438906 |
- |
KCNQ2 |
NNNCTGACCCTGATGAACTG |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000015.10 |
15 |
66029588 |
+ |
MEGF11 |
NNNCTGTGACTGATGAACGG |
NGG |
2 |
0.2812 |
Tier II |
| 6 |
NC_000017.11 |
17 |
63358549 |
- |
TANC2 |
NNNCTGACAGTGATGAACAG |
NGG |
2 |
0.2778 |
Tier II |
| 7 |
NC_000023.11 |
X |
47005718 |
- |
JADE3 |
NNNCTGACACTGCTGAACAG |
NGG |
2 |
0.1504 |
Tier II |
| 8 |
NC_000017.11 |
17 |
57628351 |
+ |
MSI2 |
NNNCTGACACTGATGACAGG |
NGG |
2 |
0.095 |
Tier II |
| 9 |
NC_000003.12 |
3 |
12314618 |
- |
PPARG |
NNNCTGACACTGTTGAACGG |
NGA |
2 |
0.0208 |
Tier II |
| 10 |
NC_000011.10 |
11 |
67458895 |
- |
CABP4 |
NNNCTCACACTGAGGAACGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000023.11 |
X |
24953194 |
- |
POLA1 |
NNNCTGACACTGAGGAAGGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000013.11 |
13 |
21319957 |
+ |
GRK6P1 |
NNNCTGACGCTGATGAACGG |
NGG |
1 |
0.6667 |
Tier III |
| 13 |
NC_000013.11 |
13 |
21319957 |
+ |
MIPEPP3 |
NNNCTGACGCTGATGAACGG |
NGG |
1 |
0.6667 |
Tier III |
| 14 |
NC_000013.11 |
13 |
21319957 |
+ |
LINC00539 |
NNNCTGACGCTGATGAACGG |
NGG |
1 |
0.6667 |
Tier III |
| 15 |
NC_000005.10 |
5 |
17783797 |
- |
LOC105374666 |
NNNCTGACACTAATGAAAGG |
NGG |
2 |
0.5026 |
Tier III |
| 16 |
NC_000001.11 |
1 |
244801404 |
+ |
LOC107985372 |
NNNCTGGCACTGATGAAAGG |
NGG |
2 |
0.3801 |
Tier III |
| 17 |
NC_000002.12 |
2 |
56640549 |
- |
LOC101927213 |
NNNCTGACACTGATCAACAG |
NGG |
2 |
0.1948 |
Tier III |
| 18 |
NC_000011.10 |
11 |
115760120 |
- |
LINC00900 |
NNNCTGACACTGATGACCCG |
NGG |
2 |
0.0791 |
Tier III |
| 19 |
NC_000011.10 |
11 |
115760120 |
- |
LINC02698 |
NNNCTGACACTGATGACCCG |
NGG |
2 |
0.0791 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
55452197 |
+ |
Grk6 |
NNNCTGACATTGATGAATGG |
NGG |
2 |
0.605 |
Tier I |
| 2 |
NC_000077.6 |
11 |
98249988 |
+ |
Cdk12 |
NNNCTGACACTGATGAACGC |
NGT |
2 |
0.0069 |
Tier I |
| 3 |
NC_000084.6 |
18 |
71832268 |
+ |
Dcc |
NNNCTGACACTGAAGAACTG |
NGG |
2 |
0.4127 |
Tier II |
| 4 |
NC_000067.6 |
1 |
181989470 |
+ |
Enah |
NNNCTAACACTGTTGAACGG |
NGG |
2 |
0.3 |
Tier II |
| 5 |
NC_000067.6 |
1 |
136522756 |
+ |
Kif14 |
NNNCAGACAGTGATGAACGG |
NGG |
2 |
0.1944 |
Tier II |
| 6 |
NC_000075.6 |
9 |
29189739 |
- |
Ntm |
NNNCTGACACTGATGATGGG |
NGG |
2 |
0.0178 |
Tier II |
| 7 |
NC_000072.6 |
6 |
84071518 |
+ |
Dysf |
NNNCTGACACTGAGGAAGGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000074.6 |
8 |
125435724 |
+ |
Sipa1l2 |
NNNTTGACACTGAGGAACGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000070.6 |
4 |
11179995 |
+ |
A630034I12Rik |
NNNCTCACACTGATGTACGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
