Construct: sgRNA BRDN0001146647

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCCAAGTTCAAGTAAGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK15 (65061)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76485

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 201807889 - CDK15 NNNCAAGTTCAAGTAAGATG NGG 0 1.0 Tier I
2 NC_000016.10 16 6770271 - RBFOX1 NNNCAAGTTTAAGTAAAATG NGG 2 0.8784 Tier II
3 NC_000008.11 8 71234508 - EYA1 NNNCAAAGTCAAGTAAGATG NGG 2 0.7333 Tier II
4 NC_000003.12 3 141533931 - RASA2 NNNAAAGATCAAGTAAGATG NGG 2 0.6737 Tier II
5 NC_000002.12 2 179585002 + ZNF385B NNNAAAGGTCAAGTAAGATG NGG 2 0.6175 Tier II
6 NC_000010.11 10 75155532 + SAMD8 NNNAAAGGTCAAGTAAGATG NGG 2 0.6175 Tier II
7 NC_000005.10 5 126951122 - MARCHF3 NNNCGAGGTCAAGTAAGATG NGG 2 0.528 Tier II
8 NC_000015.10 15 36698907 - CDIN1 NNNCAAGTTCAAGTAAGTTG NGG 1 0.5 Tier II
9 NC_000010.11 10 117130382 - VAX1 NNNCAGGTTCAAGAAAGATG NGG 2 0.4422 Tier II
10 NC_000002.12 2 86737305 - RMND5A NNNCTAGGTCAAGTAAGATG NGG 2 0.2667 Tier II
11 NC_000008.11 8 18729410 + PSD3 NNNCAAGTTCAAGTCAAATG NGG 2 0.2545 Tier II
12 NC_000002.12 2 209851102 - UNC80 NNNTAAGTTCAAGTAAGATG NAG 2 0.2074 Tier II
13 NC_000011.10 11 94418694 + MRE11 NNNCAAGTTCAATAAAGATG NGG 2 0.1857 Tier II
14 NC_000010.11 10 89244494 - LIPA NNNCAGGTTCAAGTAAGATG NAG 2 0.1852 Tier II
15 NC_000002.12 2 189683147 - ANKAR NNNCAAGATCAAGTTAGATG NGG 2 0.16 Tier II
16 NC_000008.11 8 98133325 - POP1 NNNCAATTTCAAGTAAGATG NAG 2 0.1481 Tier II
17 NC_000016.10 16 72189621 + PMFBP1 NNNGAAGTTCAAGTAAGAAG NGG 2 0.1429 Tier II
18 NC_000010.11 10 131947456 - PPP2R2D NNNCAAGTTCAAGTAGGAAG NGG 2 0.0549 Tier II
19 NC_000007.14 7 73536918 + BCL7B NNNCAAGTTCAAGTAACCTG NGG 2 0.0448 Tier II
20 NC_000003.12 3 69790575 - MITF NNNCAACTTCAAGTAAGATG NTG 2 0.0268 Tier II
21 NC_000019.10 19 13757399 + YJU2B NNNCAAGTGCAAGTAAGATG NTG 2 0.0241 Tier II
22 NC_000011.10 11 47530277 + CELF1 NNNCAAGTTCAAGGAAGAAG NGG 2 0.0 Tier II
23 NC_000006.12 6 158312601 + TULP4 NNNCAAGTTCAAGTATGATG NGA 2 0.0 Tier II
24 NC_000005.10 5 128907959 - LOC105379168 NNNAAAGGTCAAGTAAGATG NGG 2 0.6175 Tier III
25 NC_000010.11 10 88166695 + LOC101929727 NNNCCAGTTCAAATAAGATG NGG 2 0.4615 Tier III
26 NC_000002.12 2 87059430 + ANAPC1P2 NNNCTAGGTCAAGTAAGATG NGG 2 0.2667 Tier III
27 NC_000003.12 3 98724659 + ST3GAL6-AS1 NNNAAAGTTCAAGTAAGATG NGA 2 0.0585 Tier III
28 NC_000006.12 6 9022592 - LOC105374914 NNNCAAGCTCAAGTAAGATG NTG 2 0.039 Tier III
29 NC_000005.10 5 128081921 + SLC12A2-DT NNNCAAGTTCAAGTAAGATC NTG 2 0.0167 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 59258734 - Cdk15 NNNCAAGTTCAAGTAAGATG NGG 0 1.0 Tier I
2 NC_000083.6 17 17877515 + Fpr1 NNNCAAGTTCAAGTAAGAGA NGG 2 0.2344 Tier I
3 NC_000068.7 2 77552087 + Zfp385b NNNAAAGGTCAAGTAAGATG NGG 2 0.6175 Tier II
4 NC_000067.6 1 105338154 + Rnf152 NNNCAAGTTACAGTAAGATG NGG 2 0.3467 Tier II
5 NC_000086.7 X 88024679 + Il1rapl1 NNNCTAGTTCAAGTAAAATG NGG 2 0.3394 Tier II
6 NC_000068.7 2 76210563 + Pde11a NNNCAAGTTCAAACAAGATG NGG 2 0.2637 Tier II
7 NC_000070.6 4 33086460 - Gabrr2 NNNCAAGTTCAAGTCAGATA NGG 2 0.2557 Tier II
8 NC_000084.6 18 61059979 + Pdgfrb NNNCAAGGTCATGTAAGATG NGG 2 0.2444 Tier II
9 NC_000075.6 9 14713563 + Piwil4 NNNCTAGTTCAAGTGAGATG NGG 2 0.2364 Tier II
10 NC_000068.7 2 179761185 + Cdh4 NNNCATGTTCTAGTAAGATG NGG 2 0.2198 Tier II
11 NC_000081.6 15 73603795 + Slc45a4 NNNCAACTTCTAGTAAGATG NGG 2 0.2115 Tier II
12 NC_000070.6 4 135429149 - Rcan3 NNNAAAGTTCAAGTAAGAGG NGG 2 0.2105 Tier II
13 NC_000071.6 5 32892504 - Depdc5 NNNCAGGTTCAAGCAAGATG NGG 2 0.2041 Tier II
14 NC_000086.7 X 140346043 - Frmpd3 NNNCAGGTTCAAGTCAGATG NGG 2 0.1948 Tier II
15 NC_000073.6 7 127193784 + Cd2bp2 NNNCAAGTTCAAGAAAGAAG NGG 2 0.1769 Tier II
16 NC_000072.6 6 60794952 + Snca NNNCAAGTGCAAGTAAGATG NAG 2 0.1605 Tier II
17 NC_000070.6 4 116737667 + Tesk2 NNNCAAGTTGCAGTAAGATG NGG 2 0.1556 Tier II
18 NC_000078.6 12 118257558 - Sp4 NNNCAGGTTCAAGTTAGATG NGG 2 0.1429 Tier II
19 NC_000068.7 2 35620944 - Dab2ip NNNCCAGTTCAAGTCAGATG NGG 2 0.1364 Tier II
20 NC_000086.7 X 52602095 - Gpc3 NNNCAAGTGCAAGTTAGATG NGG 2 0.1238 Tier II
21 NC_000070.6 4 98594952 + Patj NNNCAAGTTCAAGCCAGATG NGG 2 0.0779 Tier II
22 NC_000080.6 14 33279299 - Arhgap22 NNNCAAGTTCAAGCCAGATG NGG 2 0.0779 Tier II
23 NC_000067.6 1 170393191 - Nos1ap NNNCAAGTTCAAGTAAGCGG NGG 2 0.0476 Tier II
24 NC_000074.6 8 58012528 - Galntl6 NNNAAAGTTCAAGTAAGATG NTG 2 0.0328 Tier II
25 NC_000078.6 12 11312366 + Smc6 NNNCAAGTTCAAGTAAGATA NGC 2 0.0208 Tier II
26 NC_000072.6 6 108180033 - Sumf1 NNNCAAGTTCAAGTATGAGG NGG 2 0.0 Tier II
27 NC_000072.6 6 3803945 - Gm33576 NNNCAAGTTCAAGTGAGATT NGG 2 0.455 Tier III
28 NC_000075.6 9 14713563 + Gm10706 NNNCTAGTTCAAGTGAGATG NGG 2 0.2364 Tier III
29 NC_000086.7 X 140346043 - Gm32113 NNNCAGGTTCAAGTCAGATG NGG 2 0.1948 Tier III
30 NC_000074.6 8 114057828 + Gm30107 NNNCAAGTTGCAGTAAGATG NGG 2 0.1556 Tier III
31 NC_000080.6 14 103812640 - Gm35197 NNNCAAGTTGCAGTAAGATG NGG 2 0.1556 Tier III
32 NC_000086.7 X 52602095 - A630012P03Rik NNNCAAGTGCAAGTTAGATG NGG 2 0.1238 Tier III
33 NC_000067.6 1 14080044 + Gm39596 NNNCAAGTTCATGTAAGCTG NGG 2 0.0635 Tier III
34 NC_000077.6 11 46183789 + Gm35624 NNNCAAGTTCAAGTCAGATG NGA 2 0.0189 Tier III
35 NC_000082.6 16 52848488 - 4930405D01Rik NNNCAAGTTCAAGTCAGATG NGA 2 0.0189 Tier III
36 NC_000069.6 3 118539792 + Gm26871 NNNCAAGTTCATGTAAGATG NGC 2 0.0074 Tier III
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Other clones with same target sequence:

(none)