Construct: sgRNA BRDN0001146648
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAATCACACAATTAAAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KDR (3791)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77756
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
55110524 |
- |
KDR |
NNNATCACACAATTAAAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
75139322 |
- |
SAMD8 |
NNNATTACACCATTAAAGCG |
NGG |
2 |
0.3714 |
Tier II |
| 3 |
NC_000006.12 |
6 |
39872745 |
+ |
DAAM2 |
NNNATCACACAACTAAAGCG |
NAG |
2 |
0.2047 |
Tier II |
| 4 |
NC_000012.12 |
12 |
194108 |
+ |
SLC6A12 |
NNNATCACACCATTAAAGAG |
NGG |
2 |
0.1714 |
Tier II |
| 5 |
NC_000008.11 |
8 |
32499282 |
+ |
NRG1 |
NNNATCACACACTTAAAGAG |
NGG |
2 |
0.1128 |
Tier II |
| 6 |
NC_000009.12 |
9 |
10595952 |
- |
PTPRD |
NNNATCACACGATTAAAGGG |
NGG |
2 |
0.0813 |
Tier II |
| 7 |
NC_000005.10 |
5 |
165060601 |
- |
LINC03000 |
NNNATCACACAATTAAAGCA |
NAG |
2 |
0.2431 |
Tier III |
| 8 |
NC_000013.11 |
13 |
18724022 |
+ |
GXYLT1P1 |
NNNTTCACACATTTAAAGCG |
NGG |
2 |
0.2121 |
Tier III |
| 9 |
NC_000021.9 |
21 |
13823975 |
+ |
GXYLT1P2 |
NNNTTCACACATTTAAAGCG |
NGG |
2 |
0.2121 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
60082548 |
- |
Wdr12 |
NNNATCACACAATTAGAGTG |
NGG |
2 |
0.0888 |
Tier I |
| 2 |
NC_000081.6 |
15 |
99394686 |
- |
Tmbim6 |
NNNATCACAAAGTTAAAGCG |
NGG |
2 |
0.6259 |
Tier II |
| 3 |
NC_000077.6 |
11 |
29529826 |
- |
Mtif2 |
NNNATCACACAAATAAAGCT |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000069.6 |
3 |
38884748 |
+ |
Fat4 |
NNNATCACACAAATAAACCG |
NGG |
2 |
0.3297 |
Tier II |
| 5 |
NC_000071.6 |
5 |
140045510 |
+ |
Mad1l1 |
NNNATCACACAATTAAAGCA |
NTG |
2 |
0.0365 |
Tier II |
| 6 |
NC_000069.6 |
3 |
38884748 |
+ |
C230034O21Rik |
NNNATCACACAAATAAACCG |
NGG |
2 |
0.3297 |
Tier III |
| 7 |
NC_000073.6 |
7 |
13179746 |
+ |
Gm4879 |
NNNATCACACAATTAGAGTG |
NGG |
2 |
0.0888 |
Tier III |
| 8 |
NC_000081.6 |
15 |
70061065 |
- |
Gm19782 |
NNNACCACACAATTATAGCG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
