Construct: sgRNA BRDN0001146649

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCAGCTTGGATGCCCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VRK2 (7444)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77232

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 58123223 + VRK2 NNNAGCTTGGATGCCCACAA NGG 0 1.0 Tier I
2 NC_000004.12 4 36058133 + ARAP2 NNNAGCTTGTATGCTCACAA NGG 2 0.0542 Tier I
3 NC_000021.9 21 29857292 + GRIK1 NNNAGCTGGGATGCCCACAA NGG 1 0.7333 Tier II
4 NC_000023.11 X 5948907 + NLGN4X NNNAGCTGGGATGTCCACAA NGG 2 0.5378 Tier II
5 NC_000001.11 1 151061369 + MLLT11 NNNGCCTTGGATGCCCACAA NGG 2 0.4911 Tier II
6 NC_000001.11 1 151064752 + MLLT11 NNNGCCTTGGATGCCCACAA NGG 2 0.4911 Tier II
7 NC_000007.14 7 140160789 + KDM7A NNNAGGCTGGATGCCCACAA NGG 2 0.3438 Tier II
8 NC_000001.11 1 112681103 - MOV10 NNNAGGTTGGATGCCCATAA NGG 2 0.3214 Tier II
9 NC_000007.14 7 132347414 + PLXNA4 NNNAGTTTGGATTCCCACAA NGG 2 0.2786 Tier II
10 NC_000007.14 7 123518006 + IQUB NNNAGCTTGAATGCCCACCA NGG 2 0.1931 Tier II
11 NC_000005.10 5 150554774 + NDST1 NNNAGCTGGGATGCCCACCA NGG 2 0.1517 Tier II
12 NC_000011.10 11 128728755 - FLI1 NNNAGCTGGGATGCCCACCA NGG 2 0.1517 Tier II
13 NC_000007.14 7 102926010 + LRRC17 NNNAGCTTGAATGCCCAGAA NGG 2 0.1244 Tier II
14 NC_000007.14 7 102926010 + FBXL13 NNNAGCTTGAATGCCCAGAA NGG 2 0.1244 Tier II
15 NC_000007.14 7 154557148 - DPP6 NNNAGCTTGGATGCCCCCGA NGG 2 0.0662 Tier II
16 NC_000017.11 17 75595996 - MYO15B NNNAGCTTGGATGCACACAC NGG 2 0.0505 Tier II
17 NC_000007.14 7 130346203 + CPA5 NNNAGCTTTGATGCTCACAA NGG 2 0.0356 Tier II
18 NC_000006.12 6 16355229 + ATXN1 NNNAGCTTGGATGCCCACAG NTG 2 0.0298 Tier II
19 NC_000009.12 9 93093807 - LOC101927993 NNNAGCCTGGATGCCCACCA NGG 2 0.1422 Tier III
20 NC_000006.12 6 1291828 - LOC105374879 NNNTGCTTGGATGCCCAGAA NGG 2 0.0848 Tier III
21 NC_000007.14 7 130346203 + LOC105375503 NNNAGCTTTGATGCTCACAA NGG 2 0.0356 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 26498928 - Vrk2 NNNAGTTTGGATGCACACAA NGG 2 0.2063 Tier I
2 NC_000074.6 8 85067648 + Gm5741 NNNAGCTGGGATGCCCACAA NGA 2 0.0509 Tier I
3 NC_000073.6 7 75548328 - Akap13 NNNAGCTGGGATGCCCACAA NGG 1 0.7333 Tier II
4 NC_000077.6 11 118824743 + Rbfox3 NNNAGCCTGGATGCCCACAA NGG 1 0.6875 Tier II
5 NC_000084.6 18 65101406 - Nedd4l NNNAGCATGGATGCCCACAG NGG 2 0.6691 Tier II
6 NC_000080.6 14 77625667 - Enox1 NNNAGCTTAAATGCCCACAA NGG 2 0.6 Tier II
7 NC_000075.6 9 119541082 + Scn5a NNNAGCTTGGATGCCCACAT NGG 1 0.6 Tier II
8 NC_000071.6 5 128185704 - Tmem132d NNNAGCTTGGAAGCCCACAT NGG 2 0.48 Tier II
9 NC_000085.6 19 37275678 - Ide NNNAGCGTGGGTGCCCACAA NGG 2 0.3824 Tier II
10 NC_000073.6 7 80074523 - Zfp710 NNNAGCCTGGATGCCCACTA NGG 2 0.3702 Tier II
11 NC_000081.6 15 57452662 - Slc22a22 NNNGGCTTGGATGCCCACGA NGG 2 0.2344 Tier II
12 NC_000073.6 7 74548043 - Slco3a1 NNNAGCTGGGTTGCCCACAA NGG 2 0.2256 Tier II
13 NC_000068.7 2 14659548 - Cacnb2 NNNGGCTTGGATGCCCACAC NGG 2 0.142 Tier II
14 NC_000067.6 1 8560177 - Sntg1 NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier II
15 NC_000078.6 12 67155625 - Mdga2 NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier II
16 NC_000080.6 14 12598376 + Cadps NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier II
17 NC_000084.6 18 49725318 - Dtwd2 NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier II
18 NC_000084.6 18 51174999 - Prr16 NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier II
19 NC_000067.6 1 92091754 + Hdac4 NNNAGCTTGGAAGCCCAGAA NGG 2 0.1067 Tier II
20 NC_000076.6 10 117021004 + Best3 NNNAGCTTGGATGTCCTCAA NGG 2 0.0978 Tier II
21 NC_000071.6 5 38812309 - Clnk NNNAGCTTGGATGCCTACCA NGG 2 0.0637 Tier II
22 NC_000071.6 5 140357087 + Snx8 NNNAGCTTGGATGCAGACAA NGG 2 0.0342 Tier II
23 NC_000070.6 4 33209037 + Srsf12 NNNAGCTTGCATGCCCACAA NTG 2 0.0156 Tier II
24 NC_000075.6 9 120123987 + Slc25a38 NNNAGCTTGGCTGCCCACAA NGC 2 0.0089 Tier II
25 NC_000078.6 12 4208987 + Cenpo NNNAGCTTGGATGCCCCCAA NTG 2 0.0069 Tier II
26 NC_000078.6 12 4208987 + Adcy3 NNNAGCTTGGATGCCCCCAA NTG 2 0.0069 Tier II
27 NC_000085.6 19 50460823 + Sorcs1 NNNAGCTTGGATGCCCAGAA NTG 2 0.0052 Tier II
28 NC_000070.6 4 68933997 + Brinp1 NNNAGCTTGGATGCCCACAA NCC 2 0.0 Tier II
29 NC_000067.6 1 180579610 - Parp1 NNNAGCTTGGATGGCCACAA NGG 1 0.0 Tier II
30 NC_000069.6 3 101410344 - Igsf3 NNNAGCTTGGCTGGCCACAA NGG 2 0.0 Tier II
31 NC_000086.7 X 149833036 + LOC100503973 NNNTGCTTGGATGCCCACAT NGG 2 0.3818 Tier III
32 NC_000073.6 7 80074523 - Gm21057 NNNAGCCTGGATGCCCACTA NGG 2 0.3702 Tier III
33 NC_000086.7 X 70010971 - 1700111N16Rik NNNAGCTTGGATTCCCACTA NGG 2 0.1615 Tier III
34 NC_000080.6 14 13254983 - Gm5087 NNNGGCTTGGATGCCCACCA NGG 2 0.1293 Tier III
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Other clones with same target sequence:

(none)