Construct: sgRNA BRDN0001146657
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCCTAGATACACCCTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R1 (5295)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77090
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
68279611 |
+ |
PIK3R1 |
NNNCCTAGATACACCCTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
110227966 |
+ |
COL4A1 |
NNNCCTAAATACACCCTCCT |
NGG |
2 |
0.7 |
Tier II |
| 3 |
NC_000003.12 |
3 |
177107695 |
+ |
TBL1XR1 |
NNNCATAGATACACCATCCG |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000019.10 |
19 |
55256920 |
+ |
PPP6R1 |
NNNCCTAGATCCACCCTCTG |
NGG |
2 |
0.1846 |
Tier II |
| 5 |
NC_000014.9 |
14 |
103056634 |
- |
CDC42BPB |
NNNCCTAGATCCACCCTCCG |
NGT |
2 |
0.0065 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
104793241 |
+ |
Cntn6 |
NNNCCTAGAAACACCCTCCT |
NGG |
2 |
0.6 |
Tier II |
| 2 |
NC_000071.6 |
5 |
27062661 |
- |
Dpp6 |
NNNCCTAGCTCCACCCTCCG |
NGG |
2 |
0.2286 |
Tier II |
| 3 |
NC_000080.6 |
14 |
60008742 |
- |
Atp8a2 |
NNNCCTAGAAACACCCTCCG |
NGA |
2 |
0.0595 |
Tier II |
| 4 |
NC_000080.6 |
14 |
114821894 |
+ |
Gm41244 |
NNNCCTAGATACACCCTGCT |
NGG |
2 |
0.0933 |
Tier III |
| 5 |
NC_000083.6 |
17 |
9757817 |
+ |
4930452A19Rik |
NNNCCTAGATACCCCTTCCG |
NGG |
2 |
0.0648 |
Tier III |
Other clones with same target sequence:
(none)
