Construct: sgRNA BRDN0001146658
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGTTGAAAACAATCGGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK3 (4140)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77719
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
103405212 |
+ |
MARK3 |
NNNGTTGAAAACAATCGGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
35653578 |
- |
RBM12 |
NNNGTTGATAACAATGGGCA |
NGG |
2 |
0.0923 |
Tier I |
| 3 |
NC_000001.11 |
1 |
232428581 |
+ |
SIPA1L2 |
NNNGTTGAAAACAATCAGAA |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000017.11 |
17 |
18351032 |
- |
SHMT1 |
NNNTTTGAAAACAATAGGCA |
NGG |
2 |
0.3636 |
Tier II |
| 5 |
NC_000002.12 |
2 |
197868039 |
+ |
PLCL1 |
NNNGTTGAAAACAAATGGCA |
NGG |
2 |
0.1781 |
Tier II |
| 6 |
NC_000023.11 |
X |
72098979 |
- |
NHSL2 |
NNNGTTGAAAAAAATGGGCA |
NGG |
2 |
0.1099 |
Tier II |
| 7 |
NC_000020.11 |
20 |
35653578 |
- |
CPNE1 |
NNNGTTGATAACAATGGGCA |
NGG |
2 |
0.0923 |
Tier II |
| 8 |
NC_000012.12 |
12 |
45365456 |
- |
ANO6 |
NNNGTTGAAAGCAATCGGCA |
NGA |
2 |
0.0451 |
Tier II |
| 9 |
NC_000016.10 |
16 |
22057475 |
- |
MOSMO |
NNNGTTGGAAACAATCGGCA |
NTG |
2 |
0.0286 |
Tier II |
| 10 |
NC_000011.10 |
11 |
89480082 |
+ |
NOX4 |
NNNGTTGAAAACAATCTGCA |
NGT |
2 |
0.004 |
Tier II |
| 11 |
NC_000013.11 |
13 |
50516930 |
- |
DLEU1 |
NNNGTTGAAAAGAATCAGCA |
NGG |
2 |
0.4148 |
Tier III |
| 12 |
NC_000002.12 |
2 |
188036698 |
- |
LINC01090 |
NNNGTTGGACACAATCGGCA |
NGG |
2 |
0.2444 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
111592713 |
+ |
Mark3 |
NNNGTTGAAAACAATCGGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000068.7 |
2 |
156096606 |
- |
Rbm12 |
NNNGTTGATAACAATGGGCA |
NGG |
2 |
0.0923 |
Tier I |
| 3 |
NC_000072.6 |
6 |
48024336 |
- |
Zfp777 |
NNNGATGAAAACAATCTGCA |
NGG |
2 |
0.125 |
Tier II |
| 4 |
NC_000068.7 |
2 |
156096606 |
- |
Cpne1 |
NNNGTTGATAACAATGGGCA |
NGG |
2 |
0.0923 |
Tier II |
| 5 |
NC_000071.6 |
5 |
137767087 |
- |
Tsc22d4 |
NNNGTTGAAAACACTCTGCA |
NGG |
2 |
0.0536 |
Tier II |
| 6 |
NC_000077.6 |
11 |
65168435 |
- |
LOC115487730 |
NNNGATGAAAACAATCAGCA |
NGG |
2 |
0.4667 |
Tier III |
Other clones with same target sequence:
(none)
