Construct: sgRNA BRDN0001146661
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCAGAAGGACCCCTTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TTBK2 (146057)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77970
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
42775140 |
- |
TTBK2 |
NNNAGAAGGACCCCTTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
39677575 |
+ |
COG6 |
NNNAGAAGGACCCATTACTG |
NGG |
2 |
0.1885 |
Tier I |
| 3 |
NC_000008.11 |
8 |
73271980 |
- |
C8orf89 |
NNNAGAAGGATCCCTTACTG |
NGG |
2 |
0.1657 |
Tier II |
| 4 |
NC_000001.11 |
1 |
150279491 |
+ |
C1orf54 |
NNNAGAAGGACTCCTTACAG |
NGA |
2 |
0.0374 |
Tier II |
| 5 |
NC_000001.11 |
1 |
150279491 |
+ |
LOC124904414 |
NNNAGAAGGACTCCTTACAG |
NGA |
2 |
0.0374 |
Tier II |
| 6 |
NC_000016.10 |
16 |
10401951 |
- |
ATF7IP2 |
NNNAGAAGGACACCTTACAG |
NTG |
2 |
0.0278 |
Tier II |
| 7 |
NC_000017.11 |
17 |
46879915 |
- |
WNT9B |
NNNAGAAGGACCCCTTGGAG |
NGG |
2 |
0.0235 |
Tier II |
| 8 |
NC_000017.11 |
17 |
46879915 |
- |
LRRC37A2 |
NNNAGAAGGACCCCTTGGAG |
NGG |
2 |
0.0235 |
Tier II |
| 9 |
NC_000023.11 |
X |
50374384 |
+ |
DGKK |
NNNAGAAGGACCCCTTACAG |
NTT |
2 |
0.0 |
Tier II |
| 10 |
NC_000002.12 |
2 |
145018923 |
+ |
TEX41 |
NNNAGAAGAACCCTTTACAG |
NGG |
2 |
0.4714 |
Tier III |
| 11 |
NC_000002.12 |
2 |
145018923 |
+ |
LOC100505498 |
NNNAGAAGAACCCTTTACAG |
NGG |
2 |
0.4714 |
Tier III |
| 12 |
NC_000020.11 |
20 |
29763502 |
- |
LOC124904968 |
NNNAGCAGGACCCCTTGCAG |
NGG |
2 |
0.0802 |
Tier III |
| 13 |
NC_000003.12 |
3 |
194778088 |
- |
LINC01968 |
NNNAGAAGGACCCCTTAGAG |
NAG |
2 |
0.0346 |
Tier III |
| 14 |
NC_000003.12 |
3 |
194778088 |
- |
LOC105374292 |
NNNAGAAGGACCCCTTAGAG |
NAG |
2 |
0.0346 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
120748440 |
- |
Ttbk2 |
NNNAGAAGGACCCCTGACTG |
NGG |
2 |
0.1864 |
Tier I |
| 2 |
NC_000080.6 |
14 |
64793025 |
+ |
Kif13b |
NNNAGAATGACCCTTTACAG |
NGG |
2 |
0.4583 |
Tier II |
| 3 |
NC_000078.6 |
12 |
29913489 |
+ |
Myt1l |
NNNAGAAGGACCCCTCACTG |
NGG |
2 |
0.359 |
Tier II |
| 4 |
NC_000073.6 |
7 |
66774901 |
- |
Cers3 |
NNNAGAAGGACTCCTTACAG |
NAG |
2 |
0.1396 |
Tier II |
| 5 |
NC_000075.6 |
9 |
99401547 |
+ |
Mras |
NNNAGAAGGAGCCCTTACTG |
NGG |
2 |
0.1346 |
Tier II |
| 6 |
NC_000077.6 |
11 |
118497626 |
- |
Rbfox3 |
NNNAGAAGGACCCCTATCAG |
NGG |
2 |
0.1212 |
Tier II |
| 7 |
NC_000072.6 |
6 |
30643089 |
- |
Cpa1 |
NNNAGAAGGAGCCCTGACAG |
NGG |
2 |
0.0865 |
Tier II |
| 8 |
NC_000077.6 |
11 |
40168558 |
+ |
Gm12132 |
NNNAGAAGGACCCCTTAAAT |
NGG |
2 |
0.3769 |
Tier III |
| 9 |
NC_000078.6 |
12 |
29913489 |
+ |
Gm46346 |
NNNAGAAGGACCCCTCACTG |
NGG |
2 |
0.359 |
Tier III |
Other clones with same target sequence:
(none)
