Construct: sgRNA BRDN0001146662

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTACACCATCACTCCACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABL2 (27)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77180

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 179121807 - ABL2 NNNACACCATCACTCCACAG NGG 0 1.0 Tier I
2 NC_000005.10 5 36300598 - RANBP3L NNNACATCATCACTCCACAG NGG 1 0.8125 Tier II
3 NC_000005.10 5 34723149 - RAI14 NNNTCACCATCACTCCACAG NGG 1 0.6364 Tier II
4 NC_000009.12 9 89105134 + SHC3 NNNAGACCATCACACCACAG NGG 2 0.3714 Tier II
5 NC_000009.12 9 123756716 - DENND1A NNNACACCCTCACACCACAG NGG 2 0.3537 Tier II
6 NC_000015.10 15 77643064 - LINGO1 NNNACACCACCACACCACAG NGG 2 0.3302 Tier II
7 NC_000015.10 15 90877788 - FURIN NNNAAACCCTCACTCCACAG NGG 2 0.3265 Tier II
8 NC_000001.11 1 30882377 + SDC3 NNNACAGCCTCACTCCACAG NGG 2 0.2689 Tier II
9 NC_000014.9 14 59697333 - RTN1 NNNACATCATCACTCTACAG NGG 2 0.25 Tier II
10 NC_000001.11 1 3384431 + PRDM16 NNNACACCGTCTCTCCACAG NGG 2 0.2222 Tier II
11 NC_000002.12 2 197460985 + COQ10B NNNACACCATCCCTCCATAG NGG 2 0.1692 Tier II
12 NC_000012.12 12 41037759 - CNTN1 NNNCCCCCATCACTCCACAG NGG 2 0.1604 Tier II
13 NC_000003.12 3 58020044 + FLNB NNNACACCATGACACCACAG NGG 2 0.1548 Tier II
14 NC_000001.11 1 161512271 - FCGR2A NNNACCCCATCCCTCCACAG NGG 2 0.1196 Tier II
15 NC_000001.11 1 161675761 - FCGR2B NNNACCCCATCCCTCCACAG NGG 2 0.1196 Tier II
16 NC_000018.10 18 6781521 + ARHGAP28 NNNACCCCATCACTCCCCAG NGG 2 0.0802 Tier II
17 NC_000011.10 11 19396978 - NAV2 NNNACACCCTCACTCCTCAG NGG 2 0.0762 Tier II
18 NC_000008.11 8 23144823 - TNFRSF10D NNNACACCATCACTCCACAG NTG 1 0.039 Tier II
19 NC_000007.14 7 31773492 + PDE1C NNNACACCATCACTTCACAC NGG 2 0.0286 Tier II
20 NC_000001.11 1 40301593 - COL9A2 NNNCCACCATCACTTCACAG NGG 2 0.0235 Tier II
21 NC_000019.10 19 43989609 - ZNF155 NNNACACCATCACTCCACAG NGC 1 0.0222 Tier II
22 NC_000005.10 5 36300598 - LOC124900962 NNNACATCATCACTCCACAG NGG 1 0.8125 Tier III
23 NC_000005.10 5 151965123 - LINC01933 NNNAGACCATCACTCAACAG NGG 2 0.6 Tier III
24 NC_000015.10 15 87503210 + LOC102724465 NNNAGACCATCACTCCATAG NGG 2 0.3857 Tier III
25 NC_000015.10 15 77643064 - LINGO1-AS1 NNNACACCACCACACCACAG NGG 2 0.3302 Tier III
26 NC_000012.12 12 56982133 + LOC390332 NNNACACCTTCACTCCAAAG NGG 2 0.3231 Tier III
27 NC_000012.12 12 56982133 + LOC124902945 NNNACACCTTCACTCCAAAG NGG 2 0.3231 Tier III
28 NC_000007.14 7 65750435 + LINC03006 NNNACACCATCACTCTACAA NGG 2 0.2885 Tier III
29 NC_000002.12 2 217208842 - LOC105373872 NNNACAGCCTCACTCCACAG NGG 2 0.2689 Tier III
30 NC_000009.12 9 93964236 - LOC124902216 NNNACACCAACACCCCACAG NGG 2 0.2449 Tier III
31 NC_000006.12 6 9945119 + OFCC1 NNNACACCATCACTCTACAT NGG 2 0.2154 Tier III
32 NC_000013.11 13 78185860 - OBI1-AS1 NNNACACCATCACACTACAG NGG 2 0.1905 Tier III
33 NC_000001.11 1 161593930 - FCGR2C NNNACCCCATCCCTCCACAG NGG 2 0.1196 Tier III
34 NC_000017.11 17 34411096 - LOC124903986 NNNACCCCATCACTCCACAG NGA 2 0.0316 Tier III
35 NC_000013.11 13 111897430 - LINC00354 NNNACTCCATCACGCCACAG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 31784568 + Abl1 NNNTCACCATCACTCCACGG NGG 2 0.2386 Tier I
2 NC_000083.6 17 33955511 + Rps18 NNNACTCCATCGCTCCACAG NGG 2 0.5159 Tier II
3 NC_000077.6 11 66368117 - Shisa6 NNNACACAATCACTCCAAAG NGG 2 0.35 Tier II
4 NC_000075.6 9 86785982 - Snap91 NNNACACCATCACTCTACAG NGG 1 0.3077 Tier II
5 NC_000067.6 1 34174054 - Dst NNNACAGCTTCACTCCACAG NGG 2 0.2824 Tier II
6 NC_000073.6 7 110955645 - Mrvi1 NNNACTCCATCAATCCACAG NGG 2 0.2747 Tier II
7 NC_000081.6 15 41493702 - Oxr1 NNNACACCATCACTCCACAG NAG 1 0.2593 Tier II
8 NC_000074.6 8 32142281 + Nrg1 NNNACAACATCACTCTACAG NGG 2 0.2308 Tier II
9 NC_000083.6 17 10531714 - Pacrg NNNACACCACCCCTCCACAG NGG 2 0.1404 Tier II
10 NC_000078.6 12 29965799 - Pxdn NNNACAGCATCACTCCCCAG NGG 2 0.083 Tier II
11 NC_000077.6 11 11819961 - Ddc NNNACACCATCACACCAGAG NGG 2 0.0825 Tier II
12 NC_000081.6 15 35142395 - Stk3 NNNACACCATCCCTCTACAG NGG 2 0.081 Tier II
13 NC_000068.7 2 152443397 + 6820408C15Rik NNNACACCACCAGTCCACAG NGG 2 0.0727 Tier II
14 NC_000071.6 5 105782389 - Lrrc8d NNNACAGCATCACTCCACAG NCG 2 0.0504 Tier II
15 NC_000080.6 14 13943450 + Gm11100 NNNACACCATCTGTCCACAG NGG 2 0.0455 Tier II
16 NC_000068.7 2 112602755 + Aven NNNACACCAACACTGCACAG NGG 2 0.0429 Tier II
17 NC_000078.6 12 52918430 + Akap6 NNNACATCATCACTCCACAG NTG 2 0.0317 Tier II
18 NC_000074.6 8 11395835 - Col4a2 NNNACACCATCACTCCACTG NGC 2 0.012 Tier II
19 NC_000070.6 4 130928586 - Laptm5 NNNACACCAGCACTCCACAG NGC 2 0.0111 Tier II
20 NC_000071.6 5 99332276 + A930011G23Rik NNNAGACCAGCACTCCACAG NGG 2 0.3 Tier III
21 NC_000067.6 1 34174054 - Gm26788 NNNACAGCTTCACTCCACAG NGG 2 0.2824 Tier III
22 NC_000083.6 17 10531714 - Gm16168 NNNACACCACCCCTCCACAG NGG 2 0.1404 Tier III
23 NC_000083.6 17 10531714 - Gm52294 NNNACACCACCCCTCCACAG NGG 2 0.1404 Tier III
24 NC_000078.6 12 70229582 - F730035M05Rik NNNACACCATCACTACCCAG NGG 2 0.0392 Tier III
25 NC_000078.6 12 70229582 - Gm38467 NNNACACCATCACTACCCAG NGG 2 0.0392 Tier III
26 NC_000068.7 2 50408027 - Gm13483 NNNACACCATCAGTCCACAG NGT 2 0.0022 Tier III
27 NC_000068.7 2 50408027 - Gm48908 NNNACACCATCAGTCCACAG NGT 2 0.0022 Tier III
Download CSV

Other clones with same target sequence:

(none)