Construct: sgRNA BRDN0001146663

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCCGATGGACTATACCGAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80178

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 8749477 - DENND2B NNNGATGGACTATGCCAAAC NGG 2 0.4356 Tier II
2 NC_000020.11 20 61472982 + CDH4 NNNGGTGGACTATACCCAAC NGG 2 0.1694 Tier II
3 NC_000003.12 3 190655831 + IL1RAP NNNGATGGACAATAACGAAC NGG 2 0.1667 Tier II
4 NC_000001.11 1 198833263 - MIR181A1HG NNNGATGCACTATACCGAAC NGT 2 0.0099 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 31962902 - Kcnmb2 NNNGATGGACTATATCCAAC NGG 2 0.0157 Tier II
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Other clones with same target sequence:

(none)