Construct: sgRNA BRDN0001146665

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTATTGCCATAAACTCCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRK (54861)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76733

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 43303604 + SNRK NNNTTGCCATAAACTCCATG NGG 0 1.0 Tier I
2 NC_000003.12 3 124298911 + KALRN NNNTTGCCATGAACTCCATG NTG 2 0.0253 Tier I
3 NC_000023.11 X 52974381 + FAM156A NNNTTGACATAAACTCCATG NGG 1 0.75 Tier II
4 NC_000010.11 10 107135001 + SORCS1 NNNTTCCCATAAACTCCATG NGG 1 0.6818 Tier II
5 NC_000011.10 11 132814745 - OPCML NNNTTACAATAAACTCCATG NGG 2 0.65 Tier II
6 NC_000006.12 6 79230305 + HMGN3 NNNTGGCTATAAACTCCATG NGG 2 0.56 Tier II
7 NC_000009.12 9 3333942 + RFX3 NNNTTTTCATAAACTCCATG NGG 2 0.5417 Tier II
8 NC_000009.12 9 35505553 + RUSC2 NNNTAACCATAAACTCCATG NGG 2 0.5 Tier II
9 NC_000001.11 1 91810513 + TGFBR3 NNNTTACCAGAAACTCCATG NGG 2 0.5 Tier II
10 NC_000001.11 1 10966213 + C1orf127 NNNTGCCCATAAACTCCATG NGG 2 0.4364 Tier II
11 NC_000013.11 13 73929342 + KLF12 NNNTTCCCAGAAACTCCATG NGG 2 0.3409 Tier II
12 NC_000020.11 20 16466847 + KIF16B NNNTTGCCAGAAGCTCCATG NGG 2 0.3261 Tier II
13 NC_000001.11 1 201054037 + CACNA1S NNNTTGCCACAAACTCAATG NGG 2 0.2489 Tier II
14 NC_000010.11 10 66614432 - CTNNA3 NNNAAGCCATAAACTCCATG NGG 2 0.2381 Tier II
15 NC_000008.11 8 17062713 + MICU3 NNNTTGCTATAAACTCCATG NAG 2 0.2269 Tier II
16 NC_000016.10 16 11131348 + CLEC16A NNNTTTCCATAAACTCCAAG NGG 2 0.1905 Tier II
17 NC_000014.9 14 56621198 + TMEM260 NNNTTGCCTTAAACTCCAAG NGG 2 0.1714 Tier II
18 NC_000022.11 22 32567479 - SYN3 NNNTTGCCATAAGCTCCATG NAG 2 0.1691 Tier II
19 NC_000012.12 12 77912043 - NAV3 NNNTTGCCATAAACTCAATG NAG 2 0.121 Tier II
20 NC_000009.12 9 21917130 + MTAP NNNTTGCCATAAACTGAATG NGG 2 0.0718 Tier II
21 NC_000019.10 19 19019071 + SUGP2 NNNTTGCCATATACTCCATG NGA 2 0.0231 Tier II
22 NC_000013.11 13 94282482 - GPC6 NNNTTCCCATAAACTCCATG NGT 2 0.011 Tier II
23 NC_000002.12 2 48768125 - STON1-GTF2A1L NNNTTGCCATAAAATCCATG NGC 2 0.0078 Tier II
24 NC_000007.14 7 138079941 + AKR1D1 NNNTTGCCATAAACTCCATG NCA 2 0.0 Tier II
25 NC_000003.12 3 81609672 - GBE1 NNNTTGCCATAAAGTCCATG NGC 2 0.0 Tier II
26 NC_000014.9 14 59325812 - DAAM1 NNNTTGCCCTAAAGTCCATG NGG 2 0.0 Tier II
27 NC_000004.12 4 184898052 + LINC01093 NNNTTGCCAAAAGCTCCATG NGG 2 0.559 Tier III
28 NC_000011.10 11 41820598 - LINC01499 NNNTTGCCATAAACTCCATC NGG 1 0.4286 Tier III
29 NC_000020.11 20 39776873 + LOC105372614 NNNTCGCCATCAACTCCATG NGG 2 0.4 Tier III
30 NC_000013.11 13 44894508 + LOC105370187 NNNTGGCCACAAACTCCATG NGG 2 0.3413 Tier III
31 NC_000010.11 10 1990380 - LOC105376346 NNNTTGCCATAAACACCTTG NGG 2 0.2895 Tier III
32 NC_000001.11 1 244007125 - LINC02774 NNNTTGCCATAAACTCCACA NGG 2 0.2586 Tier III
33 NC_000019.10 19 31900187 + LINC01837 NNNTTGCCATAAACTCCACA NGG 2 0.2586 Tier III
34 NC_000016.10 16 11131348 + LOC105371081 NNNTTTCCATAAACTCCAAG NGG 2 0.1905 Tier III
35 NC_000003.12 3 186463740 - LINC02052 NNNTTGCCATAAGCTCCACG NGG 2 0.1799 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 122137413 + Snrk NNNTTGCCATAAACTCCATG NGG 0 1.0 Tier I
2 NC_000082.6 16 34332127 - Kalrn NNNTTGCCATGAACTCCATG NTG 2 0.0253 Tier I
3 NC_000075.6 9 73987788 + Unc13c NNNTTGCAATAAACTCCATG NGG 1 0.65 Tier II
4 NC_000075.6 9 6275429 + Pdgfd NNNCTGCCACAAACTCCATG NGG 2 0.3451 Tier II
5 NC_000069.6 3 63951218 + Slc33a1 NNNTGGCCACAAACTCCATG NGG 2 0.3413 Tier II
6 NC_000080.6 14 103655164 + Slain1 NNNTTGGAATAAACTCCATG NGG 2 0.3059 Tier II
7 NC_000073.6 7 49378952 - Nav2 NNNTTGCTATAAACTCCAAG NGG 2 0.25 Tier II
8 NC_000077.6 11 75689097 + Crk NNNTTGCAATAAAATCCATG NGG 2 0.2275 Tier II
9 NC_000074.6 8 33364078 - Wrn NNNGTGCCATAAACCCCATG NGG 2 0.1705 Tier II
10 NC_000076.6 10 128687486 - Rab5b NNNTTGGCATAAAATCCATG NGG 2 0.1647 Tier II
11 NC_000068.7 2 69822509 - Klhl23 NNNTTCCCATAAACTCCATG NTG 2 0.0266 Tier II
12 NC_000080.6 14 66993196 + Bnip3l NNNTTGCCATGAACTCCATG NGC 2 0.0144 Tier II
13 NC_000073.6 7 99704357 + Slco2b1 NNNTTCCCATAAAGTCCATG NGG 2 0.0 Tier II
14 NC_000072.6 6 92174436 - Mrps25 NNNTTGCCATCAAGTCCATG NGG 2 0.0 Tier II
15 NC_000069.6 3 6137852 - Gm17934 NNNTTGCCATAAACTCCATG NGG 0 1.0 Tier III
16 NC_000086.7 X 94739456 - Gm39526 NNNTTCCCAGAAACTCCATG NGG 2 0.3409 Tier III
17 NC_000073.6 7 127455334 - B130055M24Rik NNNTTGCCATAAACTCTATG NTG 2 0.0182 Tier III
18 NC_000073.6 7 99704357 + Gm34280 NNNTTCCCATAAAGTCCATG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)