Construct: sgRNA BRDN0001146667
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGTAGCACTTTCAGAAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALK (238)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77394
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
29318368 |
+ |
ALK |
NNNTAGCACTTTCAGAAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
33707099 |
- |
ASIC2 |
NNNTAGCACTATCAGAAGCT |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000016.10 |
16 |
25712285 |
+ |
HS3ST4 |
NNNCAGCACTTTCAGAAGCT |
NGG |
2 |
0.4529 |
Tier II |
| 4 |
NC_000007.14 |
7 |
72936902 |
- |
POM121 |
NNNTAGCACTTTCAGAACTG |
NGG |
2 |
0.2198 |
Tier II |
| 5 |
NC_000023.11 |
X |
53071230 |
- |
GPR173 |
NNNCAGCACTTTCAGAAGCG |
NAG |
2 |
0.1678 |
Tier II |
| 6 |
NC_000003.12 |
3 |
132482403 |
+ |
DNAJC13 |
NNNTAGCACTTACAGAGGCG |
NGG |
2 |
0.1412 |
Tier II |
| 7 |
NC_000011.10 |
11 |
79062216 |
- |
TENM4 |
NNNTAGCACTTTCAGAGGCT |
NGG |
2 |
0.1235 |
Tier II |
| 8 |
NC_000012.12 |
12 |
66570364 |
- |
GRIP1 |
NNNTAGCACTTTCAGAGGCT |
NGG |
2 |
0.1235 |
Tier II |
| 9 |
NC_000003.12 |
3 |
99922827 |
- |
FILIP1L |
NNNTAGCATTTTGAGAAGCG |
NGG |
2 |
0.1193 |
Tier II |
| 10 |
NC_000003.12 |
3 |
99922827 |
- |
CMSS1 |
NNNTAGCATTTTGAGAAGCG |
NGG |
2 |
0.1193 |
Tier II |
| 11 |
NC_000020.11 |
20 |
61381839 |
+ |
CDH4 |
NNNTAGCACTTTGAGAAGCT |
NGG |
2 |
0.0955 |
Tier II |
| 12 |
NC_000006.12 |
6 |
42950109 |
+ |
CNPY3-GNMT |
NNNTAGCACTTTGGGAAGCG |
NGG |
2 |
0.0636 |
Tier II |
| 13 |
NC_000011.10 |
11 |
74150241 |
+ |
C2CD3 |
NNNTAGCACTTTGAGAGGCG |
NGG |
2 |
0.0241 |
Tier II |
| 14 |
NC_000023.11 |
X |
96135643 |
- |
LOC124905234 |
NNNCAGCACTTTCAGAAGTG |
NGG |
2 |
0.2986 |
Tier III |
| 15 |
NC_000014.9 |
14 |
103852958 |
+ |
PPP1R13B-DT |
NNNTAGCACTTTCAGAGGCT |
NGG |
2 |
0.1235 |
Tier III |
| 16 |
NC_000018.10 |
18 |
26733808 |
- |
LOC124904272 |
NNNTAGCACTTTCAGAAGCC |
NAG |
2 |
0.1111 |
Tier III |
| 17 |
NC_000008.11 |
8 |
83961401 |
- |
LOC124901968 |
NNNTAGCACTTTCAGAGGCC |
NGG |
2 |
0.0756 |
Tier III |
| 18 |
NC_000010.11 |
10 |
113316572 |
+ |
LOC105378490 |
NNNTAGGACTTTCATAAGCG |
NGG |
2 |
0.0672 |
Tier III |
| 19 |
NC_000002.12 |
2 |
214831202 |
- |
SNHG31 |
NNNTAGCACTTTCAGAAGCT |
NGA |
2 |
0.0486 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
71984695 |
+ |
Alk |
NNNTTGCACCTTCAGAAGCG |
NGG |
2 |
0.1939 |
Tier I |
| 2 |
NC_000072.6 |
6 |
99077379 |
+ |
Foxp1 |
NNNTAGCAGTTACAGAAGCG |
NGG |
2 |
0.4952 |
Tier II |
| 3 |
NC_000067.6 |
1 |
39422911 |
- |
Tbc1d8 |
NNNGAGCACTTTCAGAAGCT |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000083.6 |
17 |
44879653 |
+ |
Supt3 |
NNNTAGCTCTTTCAGAAGCA |
NGG |
2 |
0.4018 |
Tier II |
| 5 |
NC_000086.7 |
X |
85184196 |
- |
Dmd |
NNNAAGCACTTTCAGAAGAG |
NGG |
2 |
0.2041 |
Tier II |
| 6 |
NC_000086.7 |
X |
94196499 |
+ |
Eif2s3x |
NNNTAGCACTTTCTGAAGCG |
NGA |
2 |
0.037 |
Tier II |
| 7 |
NC_000084.6 |
18 |
25433364 |
+ |
AW554918 |
NNNTAGCACTTTCAGAAGGG |
NGC |
2 |
0.0028 |
Tier II |
| 8 |
NC_000077.6 |
11 |
68978236 |
- |
Gm32384 |
NNNTAGCACTTTCAGAAGCC |
NGA |
2 |
0.0298 |
Tier III |
Other clones with same target sequence:
(none)
