Construct: sgRNA BRDN0001146674
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATGCTCTCTAGGACGTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRSK1 (84446)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77785
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
55302793 |
- |
BRSK1 |
NNNTGCTCTCTAGGACGTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
149628813 |
+ |
ARHGEF37 |
NNNTGCTCCCTAGGACATCG |
NGG |
2 |
0.8615 |
Tier I |
| 3 |
NC_000001.11 |
1 |
147650203 |
+ |
ACP6 |
NNNTGCTCTCTAGGATGTGG |
NGG |
2 |
0.0385 |
Tier I |
| 4 |
NC_000012.12 |
12 |
2271004 |
+ |
CACNA1C |
NNNTGCTCTCTAGGACAACG |
NGG |
2 |
0.6222 |
Tier II |
| 5 |
NC_000002.12 |
2 |
105070657 |
+ |
MRPS9 |
NNNTGCTCCCTAGGACGTAG |
NGG |
2 |
0.3956 |
Tier II |
| 6 |
NC_000021.9 |
21 |
34511698 |
- |
KCNE1 |
NNNTGCTCTGTAGGACGTCC |
NGG |
2 |
0.1667 |
Tier II |
| 7 |
NC_000017.11 |
17 |
45780322 |
- |
LINC02210-CRHR1 |
NNNTGCTCTCTAGGACCTTG |
NGG |
2 |
0.1086 |
Tier II |
| 8 |
NC_000011.10 |
11 |
487262 |
+ |
PTDSS2 |
NNNTGCTCTCTAGGCTGTCG |
NGG |
2 |
0.0839 |
Tier II |
| 9 |
NC_000008.11 |
8 |
18190621 |
+ |
NAT1 |
NNNTGCTCTCCAGGACGTGG |
NGG |
2 |
0.0833 |
Tier II |
| 10 |
NC_000012.12 |
12 |
2271004 |
+ |
CACNA1C-IT3 |
NNNTGCTCTCTAGGACAACG |
NGG |
2 |
0.6222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
149657299 |
- |
Pik3cd |
NNNTGCTCTCTATGCCGTCG |
NGG |
2 |
0.0818 |
Tier I |
| 2 |
NC_000073.6 |
7 |
110474831 |
+ |
Sbf2 |
NNNGGCTCTCTAGGACGTCA |
NGG |
2 |
0.5859 |
Tier II |
| 3 |
NC_000070.6 |
4 |
86233479 |
- |
Adamtsl1 |
NNNTGCTCTCTAGGAAGTTG |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000074.6 |
8 |
93714415 |
- |
Gm26843 |
NNNTGCTCTCTAGGTAGTCG |
NGG |
2 |
0.2 |
Tier III |
| 5 |
NC_000074.6 |
8 |
93714415 |
- |
Gm30606 |
NNNTGCTCTCTAGGTAGTCG |
NGG |
2 |
0.2 |
Tier III |
| 6 |
NC_000078.6 |
12 |
112278103 |
- |
Gm36894 |
NNNTGCTCTCTAGGACTTAG |
NGG |
2 |
0.1071 |
Tier III |
Other clones with same target sequence:
(none)
