Construct: sgRNA BRDN0001146677

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGGTGACCATCTCAGAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K3 (5606)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76380

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 21300586 + MAP2K3 NNNGTGACCATCTCAGAACT NGG 0 1.0 Tier I
2 NC_000012.12 12 129078649 - TMEM132D NNNGTGACCATCACAGACCT NGG 2 0.1319 Tier I
3 NC_000005.10 5 43613058 - NNT NNNGTGATCATCTGAGAACT NGG 2 0.0 Tier I
4 NC_000004.12 4 80835066 - CFAP299 NNNGTGACATTCTCAGAACT NGG 2 0.7563 Tier II
5 NC_000009.12 9 109488973 + PTPN3 NNNGTCATCATCTCAGAACT NGG 2 0.5966 Tier II
6 NC_000010.11 10 98864155 + HPSE2 NNNGTGACCAACCCAGAACT NGG 2 0.5921 Tier II
7 NC_000001.11 1 150498269 + TARS2 NNNGTGACCATCACAGAACA NGG 2 0.3894 Tier II
8 NC_000013.11 13 101178206 - NALCN NNNGTGAACATCTCAGAATT NGG 2 0.3 Tier II
9 NC_000015.10 15 88832811 - ACAN NNNGTGAGCCTCTCAGAACT NGG 2 0.2143 Tier II
10 NC_000020.11 20 19790830 - RIN2 NNNGTGACCATCTCAGAGTT NGG 2 0.1846 Tier II
11 NC_000014.9 14 41622046 + LRFN5 NNNGTTACCATCTCTGAACT NGG 2 0.1333 Tier II
12 NC_000011.10 11 28488592 - METTL15 NNNGAGACCATCTCAGAACT NAG 2 0.1296 Tier II
13 NC_000005.10 5 88845667 + MEF2C NNNGTGAACATCTCAGAAGT NGG 2 0.0813 Tier II
14 NC_000004.12 4 175723271 + GPM6A NNNTTGACCATCTCAGGACT NGG 2 0.0642 Tier II
15 NC_000017.11 17 46518256 - ARL17A NNNGTGACCCTCTCAGTACT NGG 2 0.0444 Tier II
16 NC_000017.11 17 46518256 - LRRC37A2 NNNGTGACCCTCTCAGTACT NGG 2 0.0444 Tier II
17 NC_000017.11 17 64891707 + LRRC37A3 NNNGTGACCCTCTCAGTACT NGG 2 0.0444 Tier II
18 NC_000009.12 9 69829576 + CFAP95 NNNATGACCATCTCATAACT NGG 2 0.0 Tier II
19 NC_000014.9 14 69942087 + SMOC1 NNNGTGACCATCTCACATCT NGG 2 0.0 Tier II
20 NC_000006.12 6 44047226 - SCIRT NNNGTGAGCATCCCAGAACT NGG 2 0.5075 Tier III
21 NC_000008.11 8 40385456 + LOC105379388 NNNTTCACCATCTCAGAACT NGG 2 0.2479 Tier III
22 NC_000012.12 12 129078649 - LOC124903086 NNNGTGACCATCACAGACCT NGG 2 0.1319 Tier III
23 NC_000017.11 17 64891707 + LOC105376844 NNNGTGACCCTCTCAGTACT NGG 2 0.0444 Tier III
24 NC_000005.10 5 61701129 - LINC03122 NNNGTGACCATCTCAGAAGT NTG 2 0.0049 Tier III
25 NC_000005.10 5 32644147 + LINC02061 NNNGTGACCATCTCATAACT NAG 2 0.0 Tier III
26 NC_000020.11 20 63025590 - LOC105372718 NNNGTGACCATCTGAGCACT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 60943192 + Map2k3 NNNGTGACCATCTCAGAGCT NGG 1 0.4 Tier I
2 NC_000071.6 5 127789838 - Tmem132d NNNGTGACCATCACAGACCT NGG 2 0.1319 Tier I
3 NC_000082.6 16 96027261 - Brwd1 NNNGTCACAATCTCAGAACT NGG 2 0.5844 Tier II
4 NC_000067.6 1 63219651 + Zdbf2 NNNGGGAGCATCTCAGAACT NGG 2 0.4114 Tier II
5 NC_000067.6 1 63219651 + Gpr1 NNNGGGAGCATCTCAGAACT NGG 2 0.4114 Tier II
6 NC_000072.6 6 146371527 + Itpr2 NNNGTGACCAACTCAGATCT NGG 2 0.375 Tier II
7 NC_000077.6 11 81280354 - Asic2 NNNGTGACCACTTCAGAACT NGG 2 0.359 Tier II
8 NC_000072.6 6 29442715 - Flnc NNNGTGTCCATCACAGAACT NGG 2 0.3029 Tier II
9 NC_000070.6 4 44549808 + Pax5 NNNGTGACCATCTCAGAAAA NGG 2 0.2411 Tier II
10 NC_000082.6 16 57355683 + Cmss1 NNNCTGCCCATCTCAGAACT NGG 2 0.2316 Tier II
11 NC_000082.6 16 57355683 + Filip1l NNNCTGCCCATCTCAGAACT NGG 2 0.2316 Tier II
12 NC_000080.6 14 66090588 - Ephx2 NNNGTGACCTTCTCTGAACT NGG 2 0.1765 Tier II
13 NC_000085.6 19 57892431 - Atrnl1 NNNGTGACTATCTCAGGACT NGG 2 0.1544 Tier II
14 NC_000068.7 2 165871532 - Zmynd8 NNNGTGGCCATCTCAGCACT NGG 2 0.1246 Tier II
15 NC_000070.6 4 122858015 - Ppt1 NNNGTGAACATCTCAGACCT NGG 2 0.1238 Tier II
16 NC_000073.6 7 134866920 + Dock1 NNNGTGACCATCACAGAACG NGG 2 0.1222 Tier II
17 NC_000071.6 5 135825284 - Srrm3 NNNGTGACCAGCTCAGCACT NGG 2 0.0706 Tier II
18 NC_000082.6 16 66773896 + Cadm2 NNNGTGACTATCTCAGAACT NGA 2 0.0608 Tier II
19 NC_000085.6 19 22762061 - Trpm3 NNNGTGACCATCTCAGATCT NGC 2 0.0111 Tier II
20 NC_000076.6 10 126990526 + Ctdsp2 NNNGGGACCATCTCATAACT NGG 2 0.0 Tier II
21 NC_000084.6 18 51169500 + Prr16 NNNGTGACCATTTGAGAACT NGG 2 0.0 Tier II
22 NC_000076.6 10 22605285 - Gm40612 NNNGTGAACAACTCAGAACT NGG 2 0.4875 Tier III
23 NC_000076.6 10 22605285 - Gm46216 NNNGTGAACAACTCAGAACT NGG 2 0.4875 Tier III
24 NC_000070.6 4 101896756 - Gm12792 NNNGTGACTATCTCAGAGCT NGG 2 0.35 Tier III
25 NC_000068.7 2 10347133 - Gm13261 NNNGTGCCCCTCTCAGAACT NGG 2 0.1458 Tier III
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Other clones with same target sequence:

(none)