Construct: sgRNA BRDN0001146678
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CACCTGGTATGTTCGTATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA7 (2045)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77353
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
93411131 |
+ |
EPHA7 |
NNNCTGGTATGTTCGTATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
27772275 |
- |
CCDC25 |
NNNCTGCTATGTTTGTATCG |
NGG |
2 |
0.5042 |
Tier II |
| 3 |
NC_000008.11 |
8 |
27772275 |
- |
ESCO2 |
NNNCTGCTATGTTTGTATCG |
NGG |
2 |
0.5042 |
Tier II |
| 4 |
NC_000003.12 |
3 |
7332704 |
- |
GRM7 |
NNNCTGGTATGTTCCTGTCG |
NGG |
2 |
0.0481 |
Tier II |
| 5 |
NC_000011.10 |
11 |
96489911 |
- |
JRKL-AS1 |
NNNCTGGTATGTTCGTATCT |
NCG |
2 |
0.075 |
Tier III |
| 6 |
NC_000010.11 |
10 |
64897356 |
+ |
LOC105378336 |
NNNCTGGTATGTTCCTATGG |
NGG |
2 |
0.0341 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
28821039 |
- |
Epha7 |
NNNCTGGTATGTTCTTATCG |
NAG |
2 |
0.037 |
Tier I |
| 2 |
NC_000077.6 |
11 |
68137686 |
+ |
Stx8 |
NNNCTGGTATGCTCGTATCT |
NGG |
2 |
0.6632 |
Tier II |
| 3 |
NC_000075.6 |
9 |
61862778 |
- |
Gm34974 |
NNNCTGGTATGTGTGTATCG |
NGG |
2 |
0.1913 |
Tier III |
Other clones with same target sequence:
(none)
