Construct: sgRNA BRDN0001146680

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGGCACGGCTATTACTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK1 (1612)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76518

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 87649967 + DAPK1 NNNGCACGGCTATTACTCTG NGG 0 1.0 Tier I
2 NC_000007.14 7 138915291 + KIAA1549 NNNTCACTGCTATTACTCTG NGG 2 0.2273 Tier II
3 NC_000011.10 11 114223121 - ZBTB16 NNNGAACGGCTATCACTCTG NGG 2 0.1633 Tier II
4 NC_000016.10 16 49569681 + ZNF423 NNNGCACTGCTATTAGTCTG NGG 2 0.0962 Tier II
5 NC_000006.12 6 107077503 + BEND3 NNNTCACGGCTCTTACTCTG NGG 2 0.0957 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 60729423 + Dapk1 NNNGCATGGCTATTACTCCG NGG 2 0.2241 Tier I
2 NC_000082.6 16 36215787 - Csta3 NNNGCACAGCTATTACTCTA NGG 2 0.9375 Tier II
3 NC_000076.6 10 71040756 + Bicc1 NNNGCACGGCCTTTACTCTG NGG 2 0.2222 Tier II
4 NC_000084.6 18 50158570 + Hsd17b4 NNNGCACGGGTATTATTCTG NGG 2 0.1197 Tier II
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Other clones with same target sequence:

(none)