Construct: sgRNA BRDN0001146680
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGCACGGCTATTACTCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DAPK1 (1612)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76518
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
87649967 |
+ |
DAPK1 |
NNNGCACGGCTATTACTCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
138915291 |
+ |
KIAA1549 |
NNNTCACTGCTATTACTCTG |
NGG |
2 |
0.2273 |
Tier II |
| 3 |
NC_000011.10 |
11 |
114223121 |
- |
ZBTB16 |
NNNGAACGGCTATCACTCTG |
NGG |
2 |
0.1633 |
Tier II |
| 4 |
NC_000016.10 |
16 |
49569681 |
+ |
ZNF423 |
NNNGCACTGCTATTAGTCTG |
NGG |
2 |
0.0962 |
Tier II |
| 5 |
NC_000006.12 |
6 |
107077503 |
+ |
BEND3 |
NNNTCACGGCTCTTACTCTG |
NGG |
2 |
0.0957 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
60729423 |
+ |
Dapk1 |
NNNGCATGGCTATTACTCCG |
NGG |
2 |
0.2241 |
Tier I |
| 2 |
NC_000082.6 |
16 |
36215787 |
- |
Csta3 |
NNNGCACAGCTATTACTCTA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000076.6 |
10 |
71040756 |
+ |
Bicc1 |
NNNGCACGGCCTTTACTCTG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000084.6 |
18 |
50158570 |
+ |
Hsd17b4 |
NNNGCACGGGTATTATTCTG |
NGG |
2 |
0.1197 |
Tier II |
Other clones with same target sequence:
(none)
