Construct: sgRNA BRDN0001146685
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAACCAAGGGAACATGCCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NRBP2 (340371)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000008.11 | 8 | 143840212 | - | NRBP2 | NNNACCAAGGGAACATGCCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 207049058 | - | YOD1 | NNNAGCAAGGGAACATGCCA | NGG | 1 | 0.6 | Tier I |
3 | NC_000001.11 | 1 | 207049058 | - | PFKFB2 | NNNAGCAAGGGAACATGCCA | NGG | 1 | 0.6 | Tier II |
4 | NC_000001.11 | 1 | 240227318 | + | FMN2 | NNNATAAAGGGAACATGCCA | NGG | 2 | 0.5909 | Tier II |
5 | NC_000012.12 | 12 | 96012329 | + | LTA4H | NNNAACAAGAGAACATGCCA | NGG | 2 | 0.5333 | Tier II |
6 | NC_000004.12 | 4 | 91002936 | - | CCSER1 | NNNATCAAGGGAACATGCCG | NGG | 2 | 0.4866 | Tier II |
7 | NC_000023.11 | X | 12797774 | + | PRPS2 | NNNACCAAAGGAATATGCCA | NGG | 2 | 0.4714 | Tier II |
8 | NC_000003.12 | 3 | 101485585 | + | SENP7 | NNNATCAAGGGAATATGCCA | NGG | 2 | 0.4667 | Tier II |
9 | NC_000012.12 | 12 | 100063763 | + | BLTP3B | NNNTCCGAGGGAACATGCCA | NGG | 2 | 0.4492 | Tier II |
10 | NC_000001.11 | 1 | 54684945 | + | MROH7 | NNNTCCAAGGGAACATGTCA | NGG | 2 | 0.4091 | Tier II |
11 | NC_000012.12 | 12 | 132848601 | + | CHFR | NNNACCAAGAGAACATGCAA | NGG | 2 | 0.4 | Tier II |
12 | NC_000011.10 | 11 | 113173995 | - | NCAM1 | NNNACAAAGGTAACATGCCA | NGG | 2 | 0.3571 | Tier II |
13 | NC_000005.10 | 5 | 11201673 | + | CTNND2 | NNNTCCAAGGGAACATGACA | NGG | 2 | 0.3427 | Tier II |
14 | NC_000017.11 | 17 | 34959773 | + | CCT6B | NNNACCATGGGAATATGCCA | NGG | 2 | 0.3143 | Tier II |
15 | NC_000002.12 | 2 | 198143636 | + | PLCL1 | NNNGCCAAGGGAAAATGCCA | NGG | 2 | 0.2188 | Tier II |
16 | NC_000006.12 | 6 | 84072262 | - | MRAP2 | NNNACCAAGGGAACACTCCA | NGG | 2 | 0.1667 | Tier II |
17 | NC_000002.12 | 2 | 200648277 | - | AOX1 | NNNATCAAGGGAACATTCCA | NGG | 2 | 0.1591 | Tier II |
18 | NC_000014.9 | 14 | 57786742 | + | SLC35F4 | NNNATCAAGGGAACATTCCA | NGG | 2 | 0.1591 | Tier II |
19 | NC_000015.10 | 15 | 29217935 | - | ENTREP2 | NNNACCAAGGGAACACCCCA | NGG | 2 | 0.1569 | Tier II |
20 | NC_000006.12 | 6 | 6230915 | + | F13A1 | NNNATCAAGGGAACATCCCA | NGG | 2 | 0.1497 | Tier II |
21 | NC_000018.10 | 18 | 6068894 | - | L3MBTL4 | NNNATCAAGGGAACATCCCA | NGG | 2 | 0.1497 | Tier II |
22 | NC_000023.11 | X | 108642712 | + | COL4A5 | NNNATCAAGGGAACATCCCA | NGG | 2 | 0.1497 | Tier II |
23 | NC_000004.12 | 4 | 6415281 | + | PPP2R2C | NNNACCAATGGAACATCCCA | NGG | 2 | 0.1255 | Tier II |
24 | NC_000004.12 | 4 | 142062211 | + | INPP4B | NNNACCAAGGGAACATCCTA | NGG | 2 | 0.1086 | Tier II |
25 | NC_000010.11 | 10 | 10738481 | - | CELF2 | NNNACCAAGGGTACATGCCC | NGG | 2 | 0.0758 | Tier II |
26 | NC_000008.11 | 8 | 23321263 | + | LOXL2 | NNNACCAAGGGCACCTGCCA | NGG | 2 | 0.0718 | Tier II |
27 | NC_000003.12 | 3 | 128279866 | + | EEFSEC | NNNCCCAAGGGAACATGGCA | NGG | 2 | 0.0471 | Tier II |
28 | NC_000004.12 | 4 | 172412383 | - | GALNTL6 | NNNACCAAGAGAACATGCCA | NGC | 2 | 0.0207 | Tier II |
29 | NC_000011.10 | 11 | 94875586 | - | AMOTL1 | NNNACCAAAGGAACATGCCA | NGC | 2 | 0.0143 | Tier II |
30 | NC_000020.11 | 20 | 20457897 | + | RALGAPA2 | NNNGCCAAGGGAACATGCCA | NGT | 2 | 0.0101 | Tier II |
31 | NC_000004.12 | 4 | 141133556 | - | RNF150 | NNNACCAAGGGAACATGGCA | NGA | 2 | 0.0093 | Tier II |
32 | NC_000012.12 | 12 | 1823113 | + | CACNA2D4 | NNNACCAAGGGGAGATGCCA | NGG | 2 | 0.0 | Tier II |
33 | NC_000012.12 | 12 | 1823113 | + | LRTM2 | NNNACCAAGGGGAGATGCCA | NGG | 2 | 0.0 | Tier II |
34 | NC_000006.12 | 6 | 96288028 | + | UFL1-AS1 | NNNATCAAGAGAACATGCCA | NGG | 2 | 0.5939 | Tier III |
35 | NC_000001.11 | 1 | 54684945 | + | MROH7-TTC4 | NNNTCCAAGGGAACATGTCA | NGG | 2 | 0.4091 | Tier III |
36 | NC_000001.11 | 1 | 72756504 | - | LOC105378798 | NNNACCAAGGCAACATGACA | NGG | 2 | 0.2308 | Tier III |
37 | NC_000003.12 | 3 | 156779135 | - | LINC00886 | NNNGCCAAGGGCACATGCCA | NGG | 2 | 0.1645 | Tier III |
38 | NC_000007.14 | 7 | 52399476 | - | LOC124901810 | NNNATCAAGGGAACATTCCA | NGG | 2 | 0.1591 | Tier III |
39 | NC_000017.11 | 17 | 69742717 | - | LINC01483 | NNNATCAAGGGAACATTCCA | NGG | 2 | 0.1591 | Tier III |
40 | NC_000011.10 | 11 | 41753987 | - | LINC01499 | NNNACCAAGGGAGCATCCCA | NGG | 2 | 0.1535 | Tier III |
41 | NC_000004.12 | 4 | 113418142 | - | LOC105377374 | NNNATCAAGGGAACATCCCA | NGG | 2 | 0.1497 | Tier III |
42 | NC_000015.10 | 15 | 91455708 | - | LOC107984778 | NNNATCAAGGGAACATCCCA | NGG | 2 | 0.1497 | Tier III |
43 | NC_000006.12 | 6 | 131937379 | - | CCN2-AS1 | NNNACCAATGGAACCTGCCA | NGG | 2 | 0.1455 | Tier III |
44 | NC_000005.10 | 5 | 173654447 | - | LINC01863 | NNNACCAAGGGAACATACCA | NGA | 2 | 0.0648 | Tier III |
45 | NC_000013.11 | 13 | 18959725 | + | PHF2P2 | NNNACCAAGGGAAGATGCTA | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000081.6 | 15 | 76090913 | - | Nrbp2 | NNNACCAAGGGAACATGCCC | NGG | 1 | 0.2273 | Tier I |
2 | NC_000067.6 | 1 | 130719382 | + | Yod1 | NNNAGCAAGGGACCATGCCA | NGG | 2 | 0.1263 | Tier I |
3 | NC_000080.6 | 14 | 65952233 | + | Scara3 | NNNGCCAAGGGAACACGCCA | NGG | 2 | 0.4167 | Tier II |
4 | NC_000080.6 | 14 | 77285222 | - | Enox1 | NNNAGGAAGGGAACATGCCA | NGG | 2 | 0.3 | Tier II |
5 | NC_000077.6 | 11 | 118275126 | - | Usp36 | NNNACCAAGGGAGCAGGCCA | NGG | 2 | 0.2258 | Tier II |
6 | NC_000067.6 | 1 | 50986886 | - | Tmeff2 | NNNTCCAAGGGATCATGCCA | NGG | 2 | 0.1909 | Tier II |
7 | NC_000075.6 | 9 | 71760327 | + | Cgnl1 | NNNACCCTGGGAACATGCCA | NGG | 2 | 0.1875 | Tier II |
8 | NC_000077.6 | 11 | 33569145 | + | Gabrp | NNNACAAAGGGAACTTGCCA | NGG | 2 | 0.1857 | Tier II |
9 | NC_000069.6 | 3 | 95808146 | + | Rprd2 | NNNAGCAAGGGAACATCCCA | NGG | 2 | 0.1412 | Tier II |
10 | NC_000067.6 | 1 | 130719382 | + | Pfkfb2 | NNNAGCAAGGGACCATGCCA | NGG | 2 | 0.1263 | Tier II |
11 | NC_000077.6 | 11 | 36585682 | + | Tenm2 | NNNAGCAAGGGACCATGCCA | NGG | 2 | 0.1263 | Tier II |
12 | NC_000079.6 | 13 | 48818263 | - | Phf2 | NNNACCAAGGGAACCTGCTA | NGG | 2 | 0.1259 | Tier II |
13 | NC_000069.6 | 3 | 89566301 | + | Kcnn3 | NNNACCAAGGGACCATGCCA | NGA | 2 | 0.0146 | Tier II |
14 | NC_000074.6 | 8 | 111028216 | + | Ddx19b | NNNACCAAAGGAAGATGCCA | NGG | 2 | 0.0 | Tier II |
15 | NC_000074.6 | 8 | 111028216 | + | Aars | NNNACCAAAGGAAGATGCCA | NGG | 2 | 0.0 | Tier II |
16 | NC_000080.6 | 14 | 32926974 | - | Vstm4 | NNNACCAAGGGAACATGCCA | NCA | 2 | 0.0 | Tier II |
17 | NC_000078.6 | 12 | 108372182 | - | Eml1 | NNNACCAAGGGAAGATGCCC | NGG | 2 | 0.0 | Tier II |
18 | NC_000069.6 | 3 | 52694764 | + | Gm30235 | NNNAACAAGGGAACAAGCCA | NGG | 2 | 0.5195 | Tier III |
19 | NC_000079.6 | 13 | 56427214 | - | Gm3045 | NNNACCCAGGGAGCATGCCA | NGG | 2 | 0.2853 | Tier III |
20 | NC_000070.6 | 4 | 54875191 | + | Gm34133 | NNNACCAAGGGAACATTCCA | NGG | 1 | 0.25 | Tier III |
21 | NC_000078.6 | 12 | 56545748 | - | Gm26973 | NNNACCAAGGAAACATCCCA | NGG | 2 | 0.2353 | Tier III |
22 | NC_000081.6 | 15 | 76090913 | - | Gm41349 | NNNACCAAGGGAACATGCCC | NGG | 1 | 0.2273 | Tier III |