Construct: sgRNA BRDN0001146685

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAAACCAAGGGAACATGCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRBP2 (340371)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76456

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 143840212 - NRBP2 NNNACCAAGGGAACATGCCA NGG 0 1.0 Tier I
2 NC_000001.11 1 207049058 - YOD1 NNNAGCAAGGGAACATGCCA NGG 1 0.6 Tier I
3 NC_000001.11 1 207049058 - PFKFB2 NNNAGCAAGGGAACATGCCA NGG 1 0.6 Tier II
4 NC_000001.11 1 240227318 + FMN2 NNNATAAAGGGAACATGCCA NGG 2 0.5909 Tier II
5 NC_000012.12 12 96012329 + LTA4H NNNAACAAGAGAACATGCCA NGG 2 0.5333 Tier II
6 NC_000004.12 4 91002936 - CCSER1 NNNATCAAGGGAACATGCCG NGG 2 0.4866 Tier II
7 NC_000023.11 X 12797774 + PRPS2 NNNACCAAAGGAATATGCCA NGG 2 0.4714 Tier II
8 NC_000003.12 3 101485585 + SENP7 NNNATCAAGGGAATATGCCA NGG 2 0.4667 Tier II
9 NC_000012.12 12 100063763 + BLTP3B NNNTCCGAGGGAACATGCCA NGG 2 0.4492 Tier II
10 NC_000001.11 1 54684945 + MROH7 NNNTCCAAGGGAACATGTCA NGG 2 0.4091 Tier II
11 NC_000012.12 12 132848601 + CHFR NNNACCAAGAGAACATGCAA NGG 2 0.4 Tier II
12 NC_000011.10 11 113173995 - NCAM1 NNNACAAAGGTAACATGCCA NGG 2 0.3571 Tier II
13 NC_000005.10 5 11201673 + CTNND2 NNNTCCAAGGGAACATGACA NGG 2 0.3427 Tier II
14 NC_000017.11 17 34959773 + CCT6B NNNACCATGGGAATATGCCA NGG 2 0.3143 Tier II
15 NC_000002.12 2 198143636 + PLCL1 NNNGCCAAGGGAAAATGCCA NGG 2 0.2188 Tier II
16 NC_000006.12 6 84072262 - MRAP2 NNNACCAAGGGAACACTCCA NGG 2 0.1667 Tier II
17 NC_000002.12 2 200648277 - AOX1 NNNATCAAGGGAACATTCCA NGG 2 0.1591 Tier II
18 NC_000014.9 14 57786742 + SLC35F4 NNNATCAAGGGAACATTCCA NGG 2 0.1591 Tier II
19 NC_000015.10 15 29217935 - ENTREP2 NNNACCAAGGGAACACCCCA NGG 2 0.1569 Tier II
20 NC_000006.12 6 6230915 + F13A1 NNNATCAAGGGAACATCCCA NGG 2 0.1497 Tier II
21 NC_000018.10 18 6068894 - L3MBTL4 NNNATCAAGGGAACATCCCA NGG 2 0.1497 Tier II
22 NC_000023.11 X 108642712 + COL4A5 NNNATCAAGGGAACATCCCA NGG 2 0.1497 Tier II
23 NC_000004.12 4 6415281 + PPP2R2C NNNACCAATGGAACATCCCA NGG 2 0.1255 Tier II
24 NC_000004.12 4 142062211 + INPP4B NNNACCAAGGGAACATCCTA NGG 2 0.1086 Tier II
25 NC_000010.11 10 10738481 - CELF2 NNNACCAAGGGTACATGCCC NGG 2 0.0758 Tier II
26 NC_000008.11 8 23321263 + LOXL2 NNNACCAAGGGCACCTGCCA NGG 2 0.0718 Tier II
27 NC_000003.12 3 128279866 + EEFSEC NNNCCCAAGGGAACATGGCA NGG 2 0.0471 Tier II
28 NC_000004.12 4 172412383 - GALNTL6 NNNACCAAGAGAACATGCCA NGC 2 0.0207 Tier II
29 NC_000011.10 11 94875586 - AMOTL1 NNNACCAAAGGAACATGCCA NGC 2 0.0143 Tier II
30 NC_000020.11 20 20457897 + RALGAPA2 NNNGCCAAGGGAACATGCCA NGT 2 0.0101 Tier II
31 NC_000004.12 4 141133556 - RNF150 NNNACCAAGGGAACATGGCA NGA 2 0.0093 Tier II
32 NC_000012.12 12 1823113 + CACNA2D4 NNNACCAAGGGGAGATGCCA NGG 2 0.0 Tier II
33 NC_000012.12 12 1823113 + LRTM2 NNNACCAAGGGGAGATGCCA NGG 2 0.0 Tier II
34 NC_000006.12 6 96288028 + UFL1-AS1 NNNATCAAGAGAACATGCCA NGG 2 0.5939 Tier III
35 NC_000001.11 1 54684945 + MROH7-TTC4 NNNTCCAAGGGAACATGTCA NGG 2 0.4091 Tier III
36 NC_000001.11 1 72756504 - LOC105378798 NNNACCAAGGCAACATGACA NGG 2 0.2308 Tier III
37 NC_000003.12 3 156779135 - LINC00886 NNNGCCAAGGGCACATGCCA NGG 2 0.1645 Tier III
38 NC_000007.14 7 52399476 - LOC124901810 NNNATCAAGGGAACATTCCA NGG 2 0.1591 Tier III
39 NC_000017.11 17 69742717 - LINC01483 NNNATCAAGGGAACATTCCA NGG 2 0.1591 Tier III
40 NC_000011.10 11 41753987 - LINC01499 NNNACCAAGGGAGCATCCCA NGG 2 0.1535 Tier III
41 NC_000004.12 4 113418142 - LOC105377374 NNNATCAAGGGAACATCCCA NGG 2 0.1497 Tier III
42 NC_000015.10 15 91455708 - LOC107984778 NNNATCAAGGGAACATCCCA NGG 2 0.1497 Tier III
43 NC_000006.12 6 131937379 - CCN2-AS1 NNNACCAATGGAACCTGCCA NGG 2 0.1455 Tier III
44 NC_000005.10 5 173654447 - LINC01863 NNNACCAAGGGAACATACCA NGA 2 0.0648 Tier III
45 NC_000013.11 13 18959725 + PHF2P2 NNNACCAAGGGAAGATGCTA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 76090913 - Nrbp2 NNNACCAAGGGAACATGCCC NGG 1 0.2273 Tier I
2 NC_000067.6 1 130719382 + Yod1 NNNAGCAAGGGACCATGCCA NGG 2 0.1263 Tier I
3 NC_000080.6 14 65952233 + Scara3 NNNGCCAAGGGAACACGCCA NGG 2 0.4167 Tier II
4 NC_000080.6 14 77285222 - Enox1 NNNAGGAAGGGAACATGCCA NGG 2 0.3 Tier II
5 NC_000077.6 11 118275126 - Usp36 NNNACCAAGGGAGCAGGCCA NGG 2 0.2258 Tier II
6 NC_000067.6 1 50986886 - Tmeff2 NNNTCCAAGGGATCATGCCA NGG 2 0.1909 Tier II
7 NC_000075.6 9 71760327 + Cgnl1 NNNACCCTGGGAACATGCCA NGG 2 0.1875 Tier II
8 NC_000077.6 11 33569145 + Gabrp NNNACAAAGGGAACTTGCCA NGG 2 0.1857 Tier II
9 NC_000069.6 3 95808146 + Rprd2 NNNAGCAAGGGAACATCCCA NGG 2 0.1412 Tier II
10 NC_000067.6 1 130719382 + Pfkfb2 NNNAGCAAGGGACCATGCCA NGG 2 0.1263 Tier II
11 NC_000077.6 11 36585682 + Tenm2 NNNAGCAAGGGACCATGCCA NGG 2 0.1263 Tier II
12 NC_000079.6 13 48818263 - Phf2 NNNACCAAGGGAACCTGCTA NGG 2 0.1259 Tier II
13 NC_000069.6 3 89566301 + Kcnn3 NNNACCAAGGGACCATGCCA NGA 2 0.0146 Tier II
14 NC_000074.6 8 111028216 + Ddx19b NNNACCAAAGGAAGATGCCA NGG 2 0.0 Tier II
15 NC_000074.6 8 111028216 + Aars NNNACCAAAGGAAGATGCCA NGG 2 0.0 Tier II
16 NC_000080.6 14 32926974 - Vstm4 NNNACCAAGGGAACATGCCA NCA 2 0.0 Tier II
17 NC_000078.6 12 108372182 - Eml1 NNNACCAAGGGAAGATGCCC NGG 2 0.0 Tier II
18 NC_000069.6 3 52694764 + Gm30235 NNNAACAAGGGAACAAGCCA NGG 2 0.5195 Tier III
19 NC_000079.6 13 56427214 - Gm3045 NNNACCCAGGGAGCATGCCA NGG 2 0.2853 Tier III
20 NC_000070.6 4 54875191 + Gm34133 NNNACCAAGGGAACATTCCA NGG 1 0.25 Tier III
21 NC_000078.6 12 56545748 - Gm26973 NNNACCAAGGAAACATCCCA NGG 2 0.2353 Tier III
22 NC_000081.6 15 76090913 - Gm41349 NNNACCAAGGGAACATGCCC NGG 1 0.2273 Tier III
Download CSV

Other clones with same target sequence:

(none)