Construct: sgRNA BRDN0001146687
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCAGCATCATACTGAGCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GAK (2580)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76470
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
868650 |
+ |
GAK |
NNNAGCATCATACTGAGCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
101311802 |
- |
PPP3CA |
NNNAGCATCATACTGAGCGA |
NGG |
2 |
0.1172 |
Tier II |
| 3 |
NC_000011.10 |
11 |
108579116 |
- |
EXPH5 |
NNNAGCATCATACTGAGTGG |
NGG |
2 |
0.0804 |
Tier II |
| 4 |
NC_000007.14 |
7 |
148812407 |
- |
EZH2 |
NNNAGCATAATACTGAGCCG |
NGA |
2 |
0.0595 |
Tier II |
| 5 |
NC_000004.12 |
4 |
153308446 |
+ |
TRIM2 |
NNNAGCATCATACTGAGCAG |
NTG |
2 |
0.0167 |
Tier II |
| 6 |
NC_000002.12 |
2 |
1813035 |
- |
MYT1L |
NNNAGGATCATACTGAGCCG |
NGC |
2 |
0.0111 |
Tier II |
| 7 |
NC_000002.12 |
2 |
220429661 |
+ |
LOC105373893 |
NNNAGCATGATACTGAGCCA |
NGG |
2 |
0.5804 |
Tier III |
| 8 |
NC_000004.12 |
4 |
153308446 |
+ |
ANXA2P1 |
NNNAGCATCATACTGAGCAG |
NTG |
2 |
0.0167 |
Tier III |
| 9 |
NC_000010.11 |
10 |
64825890 |
- |
ANXA2P3 |
NNNAGCATCATACTGAGCAG |
NTG |
2 |
0.0167 |
Tier III |
| 10 |
NC_000010.11 |
10 |
64825890 |
- |
LOC105378336 |
NNNAGCATCATACTGAGCAG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
108584343 |
+ |
Gak |
NNNAGCATCATACTGAGCTG |
NGG |
1 |
0.4615 |
Tier I |
| 2 |
NC_000070.6 |
4 |
45028015 |
- |
Polr1e |
NNNAGCATCATACTCGGCCG |
NGG |
2 |
0.0524 |
Tier I |
| 3 |
NC_000086.7 |
X |
6890115 |
+ |
Dgkk |
NNNAGCAACATACTGAGCCT |
NGG |
2 |
0.56 |
Tier II |
| 4 |
NC_000068.7 |
2 |
112788924 |
- |
Ryr3 |
NNNAGCATCAAACTGAGCCC |
NGG |
2 |
0.3214 |
Tier II |
| 5 |
NC_000067.6 |
1 |
56798319 |
+ |
Satb2 |
NNNAGCATCAGACTGAGCCT |
NGG |
2 |
0.28 |
Tier II |
| 6 |
NC_000068.7 |
2 |
29791359 |
+ |
Coq4 |
NNNAGCATCATGCTCAGCCG |
NGG |
2 |
0.197 |
Tier II |
| 7 |
NC_000072.6 |
6 |
27607592 |
+ |
Grm8 |
NNNAGCATCAGACTGAGCCC |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000076.6 |
10 |
88740977 |
- |
Arl1 |
NNNAGCATCCTACTGAGCCG |
NAG |
2 |
0.0864 |
Tier II |
| 9 |
NC_000071.6 |
5 |
21982340 |
+ |
Reln |
NNNAGCATCACACTGAGCCG |
NGC |
2 |
0.0148 |
Tier II |
| 10 |
NC_000078.6 |
12 |
106031236 |
+ |
Vrk1 |
NNNAGCATCATACTGCGCTG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
