Construct: sgRNA BRDN0001146690

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTGAAAGTTGTAAGCTGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RYK (6259)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76452

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 134183020 - RYK NNNGAAAGTTGTAAGCTGCG NGG 0 1.0 Tier I
2 NC_000023.11 X 6227437 - NLGN4X NNNAAAAGTTGGAAGCTGCG NGG 2 0.45 Tier II
3 NC_000005.10 5 32287370 - MTMR12 NNNGAAAGATGTAGGCTGCG NGG 2 0.4333 Tier II
4 NC_000012.12 12 18312010 + PIK3C2G NNNGAAAGATGTAGGCTGCG NGG 2 0.4333 Tier II
5 NC_000023.11 X 152434286 - GABRA3 NNNGAAAGATGTAGGCTGCG NGG 2 0.4333 Tier II
6 NC_000021.9 21 40430458 - DSCAM NNNGAAAGATGTAAGCTGAG NGG 2 0.398 Tier II
7 NC_000007.14 7 142908179 - TRPV5 NNNGAAAGTAGTAAGCTGTG NGG 2 0.3956 Tier II
8 NC_000003.12 3 124623585 + KALRN NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier II
9 NC_000004.12 4 78839794 + BMP2K NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier II
10 NC_000005.10 5 11662472 + CTNND2 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier II
11 NC_000008.11 8 50382018 + SNTG1 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier II
12 NC_000018.10 18 53003548 + DCC NNNGAAAGTTGTAAGGTGCT NGG 2 0.1077 Tier II
13 NC_000011.10 11 104163477 - PDGFD NNNGAAAGTTGCAAGCTGCG NCG 2 0.1015 Tier II
14 NC_000003.12 3 133694684 - TF NNNGAAAGTTGTGAGCTGGG NGG 2 0.0815 Tier II
15 NC_000010.11 10 74659775 + ADK NNNGAAAGTTGTAGGCTGGG NGG 2 0.0583 Tier II
16 NC_000008.11 8 66770949 + SGK3 NNNGAAAGTAGTAAGCTGCG NGT 2 0.0138 Tier II
17 NC_000008.11 8 66770949 + C8orf44-SGK3 NNNGAAAGTAGTAAGCTGCG NGT 2 0.0138 Tier II
18 NC_000022.11 22 43273869 - SCUBE1 NNNGAAAGTTTTAAGCTGCG NGT 2 0.0062 Tier II
19 NC_000023.11 X 6227437 - LOC105373156 NNNAAAAGTTGGAAGCTGCG NGG 2 0.45 Tier III
20 NC_000010.11 10 107948105 + LINC01435 NNNGAAAGATGTAGGCTGCG NGG 2 0.4333 Tier III
21 NC_000023.11 X 52263883 - LOC105377209 NNNGAAAGATGTAGGCTGCG NGG 2 0.4333 Tier III
22 NC_000004.12 4 121512753 + LOC107986309 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
23 NC_000009.12 9 12756621 - LURAP1L-AS1 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
24 NC_000009.12 9 26103805 - LINC03106 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
25 NC_000009.12 9 106040198 + LOC107987108 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
26 NC_000012.12 12 115441494 - LOC105370003 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
27 NC_000013.11 13 87341536 - LOC105370302 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
28 NC_000020.11 20 52260259 - LOC105372666 NNNGAAAGATGTAAGCTGGG NGG 2 0.1161 Tier III
29 NC_000023.11 X 8137847 + LOC107985675 NNNGAAAGTTGTAGGCTGGG NGG 2 0.0583 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 151503844 - Camta1 NNNGAAAGTTGTGAGCTGCA NGG 2 0.6114 Tier II
2 NC_000069.6 3 81155224 - Pdgfc NNNGAAATTTGAAAGCTGCG NGG 2 0.5 Tier II
3 NC_000073.6 7 137036909 + Mgmt NNNGAGAGTTGTAAGCTGCT NGG 2 0.5 Tier II
4 NC_000072.6 6 97484884 + Frmd4b NNNGAGAGTTGTAAGCTGTG NGG 2 0.3297 Tier II
5 NC_000080.6 14 65673615 - Scara5 NNNGAAAGTTGTGAGCTGTG NGG 2 0.301 Tier II
6 NC_000070.6 4 117106614 + Ptch2 NNNGACAGTTGTAAGCTGCC NGG 2 0.1948 Tier II
7 NC_000073.6 7 113259480 + Arntl NNNGACAGTTGTAAGCTGCC NGG 2 0.1948 Tier II
8 NC_000076.6 10 12778870 - Utrn NNNGAAAGTTGTAAGCTGTG NAG 2 0.1197 Tier II
9 NC_000069.6 3 31309037 - Slc7a14 NNNGAAAGTTGTCTGCTGCG NGG 2 0.1123 Tier II
10 NC_000086.7 X 130365420 - Diaph2 NNNGAAAGTTGTAATCTGCC NGG 2 0.0612 Tier II
11 NC_000075.6 9 44963984 - Ube4a NNNGAAAGTTGTAAGCTGCC NGA 2 0.0298 Tier II
12 NC_000072.6 6 136775895 + Gucy2c NNNGAGAGTTGTAAGCTGCG NTG 2 0.0278 Tier II
13 NC_000080.6 14 56949829 + Zmym2 NNNGAAAGTTGTAAGCTGCC NTG 2 0.0167 Tier II
14 NC_000075.6 9 122004927 - Gm39463 NNNGAAAGTTGTAAGATGTG NGG 2 0.4615 Tier III
15 NC_000068.7 2 164918915 + Zfp335os NNNGAAAGTTGTGAGCTGCT NGG 2 0.4565 Tier III
16 NC_000073.6 7 113259480 + Gm33721 NNNGACAGTTGTAAGCTGCC NGG 2 0.1948 Tier III
17 NC_000069.6 3 31309037 - Gm15496 NNNGAAAGTTGTCTGCTGCG NGG 2 0.1123 Tier III
18 NC_000079.6 13 56422364 + Gm46418 NNNGAAAGTTGTCAGCTGGG NGG 2 0.0263 Tier III
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Other clones with same target sequence:

(none)