Construct: sgRNA BRDN0001146694
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTACTAGGCTGACACGACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK16 (5127)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76901
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
47224731 |
- |
CDK16 |
NNNCTAGGCTGACACGACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
12866704 |
+ |
MAST1 |
NNNCGAGGATGACACGACGG |
NGG |
2 |
0.5486 |
Tier I |
| 3 |
NC_000016.10 |
16 |
87843697 |
+ |
SLC7A5 |
NNNCTGGGCTGTCACGACGG |
NGG |
2 |
0.2381 |
Tier II |
| 4 |
NC_000007.14 |
7 |
147067969 |
- |
CNTNAP2 |
NNNCTAGGCTGACACGCCGA |
NGG |
2 |
0.1654 |
Tier II |
| 5 |
NC_000010.11 |
10 |
103720779 |
+ |
SH3PXD2A |
NNNCTAGGCTGACACGTCGG |
NAG |
2 |
0.0346 |
Tier II |
| 6 |
NC_000007.14 |
7 |
31102480 |
- |
ADCYAP1R1 |
NNNCTAGGCTGACAGCACGG |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
8219906 |
- |
Kctd6 |
NNNCTGGGCTGACACGAAGG |
NGG |
2 |
0.3846 |
Tier II |
Other clones with same target sequence:
(none)
