Construct: sgRNA BRDN0001146703

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGTGGGAGATACTGATGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75544

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 140735431 - PTK2 NNNTGGGAGATACTGATGCA NGG 0 1.0 Tier I
2 NC_000017.11 17 4064664 + ZZEF1 NNNTGGGAGATACTGAGGCT NGG 2 0.0706 Tier I
3 NC_000006.12 6 155136825 + TIAM2 NNNTGGAAGATACTGATTCA NGG 2 0.6667 Tier II
4 NC_000006.12 6 17681815 - NUP153 NNNTAGGAGAAACTGATGCA NGG 2 0.65 Tier II
5 NC_000001.11 1 167678510 - RCSD1 NNNTGGGAATTACTGATGCA NGG 2 0.5672 Tier II
6 NC_000012.12 12 29580372 - TMTC1 NNNTAGGAGATACAGATGCA NGG 2 0.5365 Tier II
7 NC_000010.11 10 28287789 - MPP7 NNNTGGGAAATACTGATTCA NGG 2 0.4286 Tier II
8 NC_000001.11 1 76362767 + ST6GALNAC3 NNNTGGGACACACTGATGCA NGG 2 0.359 Tier II
9 NC_000002.12 2 141163606 + LRP1B NNNTGGGAGATACTGAATCA NGG 2 0.3556 Tier II
10 NC_000018.10 18 79085274 - ATP9B NNNTGGGAGATACTGAATCA NGG 2 0.3556 Tier II
11 NC_000015.10 15 86808973 + AGBL1 NNNTGTGAGATACTGATGAA NGG 2 0.2857 Tier II
12 NC_000003.12 3 45738051 + SACM1L NNNTGGGAGATTCTGATGCT NGG 2 0.2 Tier II
13 NC_000005.10 5 128482267 + FBN2 NNNTGGGAGAAACTGATGCA NAG 2 0.1944 Tier II
14 NC_000016.10 16 47126642 + NETO2 NNNTGGGTGATAATGATGCA NGG 2 0.1648 Tier II
15 NC_000002.12 2 109522724 - RANBP2 NNNTGGGAGATGCTGATGCC NGG 2 0.1641 Tier II
16 NC_000014.9 14 65364009 + FUT8 NNNTGGGTGATTCTGATGCA NGG 2 0.1429 Tier II
17 NC_000011.10 11 14730200 - PDE3B NNNTGGGAGATAATGATGCA NAG 2 0.0997 Tier II
18 NC_000011.10 11 35418106 + SLC1A2 NNNTGGGAGATACTGGTCCA NGG 2 0.0916 Tier II
19 NC_000001.11 1 25685752 + MAN1C1 NNNTGGGAGAAACTGAGGCA NGG 2 0.0882 Tier II
20 NC_000007.14 7 105698065 + ATXN7L1 NNNTGGGAGATACAGAGGCA NGG 2 0.0728 Tier II
21 NC_000007.14 7 96649981 - SEM1 NNNTGGGATATACTGATGGA NGG 2 0.0667 Tier II
22 NC_000012.12 12 50662614 - DIP2B NNNTGGGAGATACTGATGTA NGA 2 0.0321 Tier II
23 NC_000005.10 5 34186752 - C1QTNF3 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier II
24 NC_000005.10 5 37482830 + WDR70 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier II
25 NC_000005.10 5 69906499 + LOC124900996 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier II
26 NC_000023.11 X 105939738 + NRK NNNTGGGAGATTCTGATGCA NTG 2 0.013 Tier II
27 NC_000002.12 2 182968408 + NCKAP1 NNNTGGGAGATACTCCTGCA NGG 2 0.0 Tier II
28 NC_000005.10 5 70526068 - LOC124900995 NNNTGGGAGATACTGATGCA NCC 2 0.0 Tier II
29 NC_000005.10 5 71212006 - LOC124900994 NNNTGGGAGATACTGATGCA NCC 2 0.0 Tier II
30 NC_000005.10 5 71230032 - LOC124900993 NNNTGGGAGATACTGATGCA NCC 2 0.0 Tier II
31 NC_000015.10 15 45271165 + SLC28A2 NNNTTGGAGATACTGCTGCA NGG 2 0.0 Tier II
32 NC_000023.11 X 15731850 - CA5BP1-CA5B NNNTGGGAGAAGCTGATGCA NGG 2 0.5417 Tier III
33 NC_000005.10 5 164801582 + LINC03000 NNNCGGGAGATGCTGATGCA NGG 2 0.4673 Tier III
34 NC_000002.12 2 78437598 + LOC124906027 NNNTGGGAGATACTGAATCA NGG 2 0.3556 Tier III
35 NC_000005.10 5 69649951 - GUSBP3 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
36 NC_000005.10 5 70211642 - GUSBP14 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
37 NC_000005.10 5 70229692 - GUSBP14 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
38 NC_000005.10 5 70504783 - GUSBP15 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
39 NC_000005.10 5 70781565 + GUSBP16 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
40 NC_000005.10 5 100385571 - GUSBP19 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
41 NC_000006.12 6 57934165 - GUSBP4 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
42 NC_000006.12 6 57934165 - LINC00680-GUSBP4 NNNTGGGAGATACTGATGCA NGC 1 0.0222 Tier III
43 NC_000005.10 5 21487423 + GUSBP1 NNNCGGGAGATACTGATGCA NGC 2 0.0144 Tier III
44 NC_000002.12 2 39970457 + SLC8A1-AS1 NNNTGGGTGATACTGATGCA NGT 2 0.0069 Tier III
45 NC_000006.12 6 26893055 - GUSBP2 NNNTGGGAGATAGTGATGCA NGC 2 0.003 Tier III
46 NC_000006.12 6 26893055 - POM121L6P NNNTGGGAGATAGTGATGCA NGC 2 0.003 Tier III
47 NC_000005.10 5 70526068 - GUSBP15 NNNTGGGAGATACTGATGCA NCC 2 0.0 Tier III
48 NC_000005.10 5 71230032 - GUSBP17 NNNTGGGAGATACTGATGCA NCC 2 0.0 Tier III
49 NC_000006.12 6 18572885 - MIR548A1HG NNNTGGGAGATGCTGTTGCA NGG 2 0.0 Tier III
50 NC_000015.10 15 45271165 + SLC28A2-AS1 NNNTTGGAGATACTGCTGCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 73257214 - Ptk2 NNNTGGGAGATACTGATGCA NGG 0 1.0 Tier I
2 NC_000085.6 19 59895723 + Rab11fip2 NNNTGGAAGAGACTGATGCA NGG 2 0.4 Tier II
3 NC_000080.6 14 65689489 + Scara5 NNNTGTGAGATACTGATGCT NGG 2 0.4 Tier II
4 NC_000082.6 16 58998687 - Olfr183 NNNTCGGAGAGACTGATGCA NGG 2 0.3143 Tier II
5 NC_000083.6 17 52956930 - Kcnh8 NNNTGGGGGAGACTGATGCA NGG 2 0.2933 Tier II
6 NC_000072.6 6 113432378 + Cidec NNNTGGGAGAGACAGATGCA NGG 2 0.2476 Tier II
7 NC_000083.6 17 80159047 + Galm NNNTGGGAGATGCTGATGCA NAG 2 0.1872 Tier II
8 NC_000081.6 15 11871305 - Npr3 NNNTGGGAGAGTCTGATGCA NGG 2 0.1333 Tier II
9 NC_000070.6 4 132860110 - Stx12 NNNTGGGAGAGACTCATGCA NGG 2 0.1091 Tier II
10 NC_000073.6 7 76256514 + Agbl1 NNNTGGCAGATAGTGATGCA NGG 2 0.0938 Tier II
11 NC_000078.6 12 102350311 + Rin3 NNNTGGGAGAAACTGAGGCA NGG 2 0.0882 Tier II
12 NC_000079.6 13 116959481 + Parp8 NNNTGGGAGACACTGAGGCA NGG 2 0.0784 Tier II
13 NC_000086.7 X 157485217 - Sms NNNTGGGAGATACTGAGGTA NGG 2 0.0543 Tier II
14 NC_000081.6 15 10206992 + Prlr NNNTGGGAGATACTCAGGCA NGG 2 0.0321 Tier II
15 NC_000077.6 11 113383278 - Slc39a11 NNNTGGGAGCTACTGATGCA NGA 2 0.0231 Tier II
16 NC_000079.6 13 45727992 - Atxn1 NNNTGGGAGATAGTGATGGA NGG 2 0.017 Tier II
17 NC_000073.6 7 31291660 - Scgb1b2 NNNTGGGATATACTGATGCA NGC 2 0.0119 Tier II
18 NC_000073.6 7 31375749 + Scgb1b3 NNNTGGGATATACTGATGCA NGT 2 0.0086 Tier II
19 NC_000073.6 7 32101010 + Scgb1b10 NNNTGGGATATACTGATGCA NGT 2 0.0086 Tier II
20 NC_000073.6 7 32836711 + Scgb1b15 NNNTGGGATATACTGATGCA NGT 2 0.0086 Tier II
21 NC_000073.6 7 33063606 + Scgb1b17 NNNTGGGATATACTGATGCA NGT 2 0.0086 Tier II
22 NC_000078.6 12 13241642 + Ddx1 NNNTGGGAGATACTGAGGCA NGA 2 0.0082 Tier II
23 NC_000085.6 19 43071218 + Hpse2 NNNTTGGAGATACTGATGCA NGC 2 0.0067 Tier II
24 NC_000071.6 5 98482553 + Cfap299 NNNTAGGAGATACTGTTGCA NGG 2 0.0 Tier II
25 NC_000076.6 10 79855345 - Ptbp1 NNNTGGGAGATACGGATGCC NGG 2 0.0 Tier II
26 NC_000077.6 11 108877353 - Gm46298 NNNTGGGAGATACAGATGAA NGG 2 0.2653 Tier III
27 NC_000077.6 11 72940255 + Gm34704 NNNTGGGAGAAACTGATGGA NGG 2 0.0938 Tier III
28 NC_000073.6 7 33635301 + Scgb2b23-ps NNNTGGGATATACTGATGCA NGT 2 0.0086 Tier III
29 NC_000071.6 5 98482553 + Gm34475 NNNTAGGAGATACTGTTGCA NGG 2 0.0 Tier III
30 NC_000070.6 4 106243406 + Gm12724 NNNTGGGAGATACTGCTGCA NGC 2 0.0 Tier III
31 NC_000068.7 2 173853797 + Gm14371 NNNTGGGAGGTACTGTTGCA NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)