Construct: sgRNA BRDN0001146704
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGCTCAGAAAGAATCCGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG3 (53632)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77294
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
218830828 |
- |
PRKAG3 |
NNNGCTCAGAAAGAATCCGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
32313257 |
- |
CMTM8 |
NNNACTCAGAAAGAATCCGT |
NGG |
1 |
0.9 |
Tier II |
| 3 |
NC_000011.10 |
11 |
40927495 |
- |
LRRC4C |
NNNACTCAGAAAGAATCTGT |
NGG |
2 |
0.5786 |
Tier II |
| 4 |
NC_000005.10 |
5 |
66907362 |
+ |
MAST4 |
NNNACTCAGAAAGAATCAGT |
NGG |
2 |
0.4846 |
Tier II |
| 5 |
NC_000001.11 |
1 |
168697313 |
- |
DPT |
NNNGCTCAGAAAGAGTCAGT |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000017.11 |
17 |
11873325 |
- |
DNAH9 |
NNNTCTGAGAAAGAATCCGT |
NGG |
2 |
0.1711 |
Tier II |
| 7 |
NC_000001.11 |
1 |
116381867 |
+ |
ATP1A1 |
NNNGCTCAGAGAGAATCCGT |
NAG |
2 |
0.1685 |
Tier II |
| 8 |
NC_000016.10 |
16 |
21804680 |
- |
RRN3P1 |
NNNGCTCAGGAAGAATCCGC |
NGG |
2 |
0.0505 |
Tier III |
| 9 |
NC_000016.10 |
16 |
29092282 |
+ |
RRN3P2 |
NNNGCTCAGGAAGAATCCGC |
NGG |
2 |
0.0505 |
Tier III |
| 10 |
NC_000008.11 |
8 |
83686985 |
- |
LOC105375932 |
NNNGCTCAGAAAGAATGTGT |
NGG |
2 |
0.0378 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
60235284 |
+ |
Nbeal1 |
NNNGCTCAGAAGAAATCCGT |
NGG |
2 |
0.6667 |
Tier I |
| 2 |
NC_000071.6 |
5 |
67716788 |
+ |
Atp8a1 |
NNNGCTCGGAAAGAAACCGT |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000069.6 |
3 |
124422865 |
- |
1700006A11Rik |
NNNGCTCAGAAAGAATCAGT |
NGG |
1 |
0.5385 |
Tier II |
| 4 |
NC_000073.6 |
7 |
87885022 |
+ |
Grm5 |
NNNGCTCTGAAAGAATCCGT |
NGG |
1 |
0.4286 |
Tier II |
| 5 |
NC_000068.7 |
2 |
71515386 |
+ |
Metap1d |
NNNGCTCAGAAATAAACCGT |
NGG |
2 |
0.2727 |
Tier II |
| 6 |
NC_000081.6 |
15 |
53879587 |
+ |
Samd12 |
NNNTCTCAGAAAGAACCCGT |
NGG |
2 |
0.2424 |
Tier II |
| 7 |
NC_000077.6 |
11 |
20063450 |
- |
Actr2 |
NNNGCTCAGAAAGAATCAGG |
NGG |
2 |
0.095 |
Tier II |
| 8 |
NC_000072.6 |
6 |
87888734 |
+ |
Copg1 |
NNNGCTCAGAAAGAATGCTT |
NGG |
2 |
0.0392 |
Tier II |
| 9 |
NC_000068.7 |
2 |
164604587 |
- |
Wfdc8 |
NNNGCTCAGTAAGAATCCGT |
NTG |
2 |
0.0344 |
Tier II |
| 10 |
NC_000072.6 |
6 |
67882088 |
- |
Igk |
NNNGCTCAGAAAGAATCAGG |
NGG |
2 |
0.095 |
Tier III |
| 11 |
NC_000072.6 |
6 |
67882088 |
- |
Gm9729 |
NNNGCTCAGAAAGAATCAGG |
NGG |
2 |
0.095 |
Tier III |
| 12 |
NC_000073.6 |
7 |
7095260 |
- |
Gm6828 |
NNNGCTCAGAAAGAATCAGG |
NGG |
2 |
0.095 |
Tier III |
Other clones with same target sequence:
(none)
